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TNF antibody (PA1079) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 192 Publications

pa1079 tnf primary antibodies fcm testing 3

Flow Cytometry analysis of CACO-2 cells using anti-TNF alpha antibody (PA1079).
Overlay histogram showing CACO-2 cells stained with PA1079 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNF alpha Antibody (PA1079, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-TNF alpha Antibody Picoband®

Name: Anti-TNF alpha Antibody Picoband®
Brand: Boster Bio
SKU: PA1079
Price: 370 USD
Availability: InStock
Rating: 100 (4 reviews)

TNF antibody

192 Publications|

4 Reviews

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IHC, WB

Tested Applications

TNF antibody was positively detected by WB in:

human U937 whole cell, rat spleen tissue, rat C6 whole cell, mouse spleen tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

TNF antibody was positively detected by IHC in:

human B lymphocytic tumor tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

TNF antibody was positively detected by FC in:

CACO-2 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Mouse

Tested

Monkey

Predicted

Bovine

Predicted

Dog

Predicted

Sheep

Predicted

Product Information

Product Name

Anti-TNF alpha Antibody Picoband®
View all TNF Antibodies

Form

Lyophilized

Uniprot

P01375

Gene ID

7124

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TNF alpha, different from the related mouse sequence by five amino acids, and rat sequence by seven amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

25 kDa

Calculated Molecular Weight

25.6 kDa

Background of TNF

TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Cite This Product

Anti-TNF alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1079)

Protocols

Western Blot protocol for TNF antibody

Review Protocol

Immunohistochemistry (IHC) protocol for TNF antibody

Review Protocol

Flow Cytometry (FC) protocol for TNF antibody

Review Protocol

Publications

Study on the role and mechanism of P2X4 receptor in allergic contact dermatitis in mice

Published: 2026-08-28

Journal: NEUROPHARMACOLOGY

Application: WB

Impact Factor: 4.70

Species: Mouse

PMID: 42660283

SKU	PA1079
Application	Western Blot
Sample	PC-12 (rat adrenal pheochromocytoma cells, highly differentiated)
Sample Processing Description	① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	TNF alpha Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. TNFα is a typical pro-inflammatory factor. The experimental results showed a clear target band. After modeling, the total TNFα protein expression level was upregulated, and after drug treatment, the expression level decreased, which was consistent with theory and expectations.

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Anti-TNF alpha Antibody Picoband®

TNF antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Study on the role and mechanism of P2X4 receptor in allergic contact dermatitis in mice

Integrative microbiome and functional profiling identify Streptococcus anginosus as a pro-tumorigenic driver in nasopharyngeal carcinoma

IL‐7R‐Enriched Extracellular Vesicles From the Thymus Drive Colitis via Promoting Neutrophil Extracellular Trap Formation

Sustained Intra-Articular Delivery of Triple Therapeutics Using a Phase-Transition Phospholipid-Based Gel for Effective Treatment of Gouty Arthritis

Diabetic Wound Healing Enabled by a Tri-Functional MAX Phase via Cu Doping

Resveratrol suppresses GATA4/Sortilin pathway to attenuate vascular smooth muscle cell senescence-associated secretory phenotype and vascular aging-related atherosclerosis

Neuregulin 4 improves white adipose tissue inflammation in mice with polycystic ovary syndrome

Orally Administered Self-Microemulsifying Celastrol Alleviates Rheumatoid Arthritis by Modulating the Expression of TNF-α

Diacerein Disrupts Testicular Homeostasis: The Essential Role of Basal Cytokines in Maintaining Sertoli Cell Integrity and the Immune Microenvironment

Multi-omics Analysis Reveals the Protection of a Quadruple Probiotic Mixture in Experimental Autoimmune Hepatitis

S100A9 deletion ameliorates acute lung injury by attenuating excessive neutrophil activation via activating the NRF2/HO-1 signaling axis

Ultrasound-Excited Injectable Piezoelectric Composite Hydrogels for Enhancing Diabetic Flap Survival via Synergistic Redox Homeostasis and Angiogenesis

SARS-CoV-2 Triggers an NF-kB-Driven Proliferative Response in Epididymal Clear Cells of K18-hACE2 Mice

The novel GLP-1/GIP dual receptor agonist DA5-CH is superior to tirzepatide and exendin-4 in the 6-OHDA Parkinson rat model

Tissue-specific inflammation induces cell state plasticity with oncogenic addiction in mucosal melanoma

Comparative Biocompatibility of Portland-Based Endodontic Cements Containing Niobium and/or Bismuth Oxides in Rat Subcutaneous Tissue

Eupatilin Ameliorates Sepsis-Induced Myocardial Injury by Targeting the KAT5-H3K27la Epigenetic Axis

Brain-Targeted RVG-Liposomal Melatonin Ameliorates Manganese Neurotoxicity by Enhancing Neurogenesis and Modulating Systemic Amino Acid Profiles

Ptbp2 Alleviates Neuroinflammation and Blood–brain Barrier Disruption via Modulating Microglial Polarization in Ischemic Stroke

A Melittin-Derived Lead Compound Ameliorates Severe Acute Pancreatitis by Restoring Oxidative Homeostasis and Macrophage Metabolism

Low-intensity pulsed ultrasound ameliorates partial infraorbital nerve ligation-induced trigeminal neuropathic pain through inhibiting Schwann cell Pannexin 1 channel

Sleep deprivation attenuates acute ischemic stroke-induced hippocampal neuronal injury via the IL-38/NF-κB axis

An intelligent bacterial capture nanodrug enhances immunotherapy for implant-associated infections by inducing cuproptosis-like death in bacteria

Single—cell RNA sequencing identifies PD—L1 + mesenchymal stem cells with enhanced immunomodulatory capacity and alleviated the degree of ectopic new bone formation in ankylosing spondylitis

Discovery of pinoresinol dimethyl ether as a natural PDE4 inhibitor with anti-psoriatic effects

Hierarchical Glucose-Sensitive Hydrogel for Anti-IL-17 Antibody Delivery Enhances Alveolar Bone Regeneration in Diabetic Conditions

Geniposidic Acid from Ginger-processed Eucommiae Cortex Alleviates Rheumatoid Arthritis by Modulating Macrophage Lactylation Induced by Exosomes from Inflammatory Fibroblast-Like Synoviocytes

In vivo anti-inflammatory, analgesic activities and phytochemical profile of Hu Lisan ethanol extract

Esketamine relieves depressive-like behaviors in MPTP-induced Parkinson disease mice via GPR109A-dependent reduction of neuroinflammation

pH-responsive hydrogel system loaded with curcumin-preconditioned mesenchymal stem cell exosomes for enhanced diabetic wound healing in orthopedic applications

Tanshinone IIA and hydroxy safflower yellow A reduce cerebral injury via TLR4/NF-κB pathway in rats

Shilajit extract (ZhaXun) protects against acetaminophen-induced liver injury via modulation of the NF-κB/AKT/Caspase-3 axis

Integrated network pharmacology and transcriptomics analysis elucidated the underlying mechanisms by which Pegaeophyton scapiflorum alleviated LPS-induced acute lung injury

miR-708-5p Attenuates Osteoarthritis Progression via Multi-Target Modulation of the NOX4/NF-κB Axis and Cartilage Homeostasis

Stem cell secretome armed magneto-actuated micromotors as spatio-temporal manipulators for wound healing acceleration

Taraxacum mongolicum Hand.-Mazz. derived extracellular vesicles alleviate mastitis via NLRP3 inflammasome and NF-κB/MAPK pathways

Aqueous extract of Saposhnikovia divaricata root alleviates rheumatoid arthritis by acting on TNF-α and RAGE signaling pathways

Dynamic CYP2E1 expression and metabolic activity changes in male rats during immune liver injury and sex differences in alcohol metabolism

Therapeutic Impact of Polysaccharide from Chinese Yam (Dioscorea opposita Thunb) on DSS-Induced Ulcerative Colitis in Mice

Zwitterionic Brush-Grafted Interfacial Bio-Lubricant Evades Complement C3-Mediated Macrophage Phagocytosis for Osteoarthritis Therapy

Arsenic exposure activates microglia, inducing neuroinflammation and promoting the occurrence and development of Alzheimer's disease-like neurodegeneration in mice

Ca2+ crosslinked gelatin-sodium alginate film loaded with berberine hydrochloride that can effectively promote wound healing of MRSA infection

Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study

Enhanced activation of retinal progenitor cells by zinc silicate bioceramics for effective functional restoration in retinal degenerative diseases

Investigating the molecular mechanism of epimedium herb in treating rheumatoid arthritis through network pharmacology, molecular docking, and experimental validation

Bioactive Glycyrrhizic Acid Ionic Liquid Self-Assembled Nanomicelles for Enhanced Transdermal Delivery of Anti-Photoaging Signal Peptides

A targeted and synergetic nano-delivery system against Pseudomonas aeruginosa infection for promoting wound healing

Mechanism of S100A9-mediated astrocyte activation via TLR4/NF-κB in Parkinson’s disease

Hyperbaric oxygen therapy prevents epithelial atrophy in distal tubules and TGF-β1 overexpression in diabetic rat kidneys

Two birds with one stone: Natural polyphenols boosted periodontitis treatment of chlorhexidine via reducing toxicity and regulating microenvironments

Corynebacterium parakroppenstedtii secretes a novel glycolipid to promote the development of granulomatous lobular mastitis

Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12 promote infected wound healing via regulation of the wound microenvironment

DJ-1 regulates astrocyte activation through miR-155/SHP-1 signaling in cerebral ischemia/reperfusion injury

Semaglutide Alleviates Ovary Inflammation via the AMPK/SIRT1/NF‑κB Signaling Pathway in Polycystic Ovary Syndrome Mice

FGF20 modulates gut microbiota to mitigate dextran sodium sulfate-induced ulcerative colitis in mouse models

Up-regulation of IL-1β and sPLA2-III in the medial prefrontal cortex contributes to orofacial and somatic hyperalgesia induced by malocclusion via glial-neuron crosstalk

Reduction of TRPV1 expression on neurons due to downregulation of P2X7R in neonatal rat dorsal root ganglion satellite glial cells under co-culture conditions

Melatonin prevents bone loss in osteoporotic rats with valproic acid treatment by anti-inflammatory and anti-oxidative stress

Loss-of-function mutations in Keratin 32 gene disrupt skin immune homeostasis in pityriasis rubra pilaris

Melatonin modulates TLR4/MyD88/NF-κB signaling pathway to ameliorate cognitive impairment in sleep-deprived rats

Using broadly targeted plant metabolomics technology combined with network pharmacology to explore the mechanism of action of the Yishen Gushu formula in the treatment of postmenopausal osteoporosis in vivo

The Role of Grifola frondosa Polysaccharide in Preventing Skeletal Muscle Atrophy in Type 2 Diabetes Mellitus

“Butterfly-effect” nano-platforms for cascade immune amplification and tumor clearance through multi-modal therapy and immune sensitization

Anthrahydroquinone‑2,6‑disulfonate attenuates PQ‑induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of the PI3K/AKT/eNOS pathway

The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson’s disease

Cu+/Ca2+ Dual-Overload Strategy Assisted by Metabolic-Symbiotic-Destruction for Tumor Apoptosis and Tumor Immune Activation

Oridonin, a small molecule inhibitor of cancer stem cell with potent cytotoxicity and differentiation potential