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- Table of Contents
NKX2-5 antibody (PB9293) performs well in WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Anti-NKX2 Picoband antibody, PB9293, Western blotting
All lanes: Anti NKX2 (PB9293) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
All lanes: Anti NKX2 (PB9293) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
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Anti-Nkx2.5/NKX2-5 Antibody Picoband®
NKX2-5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | WB |
Tested Applications
NKX2-5 antibody was positively detected by WB in:
Mouse Spleen Tissue, Mouse Cardiac Muscle Tissue, HELA Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Mouse
Tested
Human
Predicted
Monkey
Predicted
Rabbit
Predicted
Product Information
Product Name
Anti-Nkx2.5/NKX2-5 Antibody Picoband®
View all NKX2-5 Antibodies
View all NKX2-5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5, different from the related mouse and rat sequences by five amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
34.9 kDa
Background of NKX2-5
Homeobox protein Nkx-2.5, also known as NKX2E or CSX is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2.5 and Tbx5 directly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. The cardiac homeobox protein Nkx2.5 is essential in cardiac development, and mutations in CSX (which encodes Nkx2.5) cause various congenital heart malformations.
Cite This Product
Anti-Nkx2.5/NKX2-5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9293)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Rabbit
90%
High Homology
Mouse
83%
Moderate Homology
Rat
83%
Moderate Homology
Cow
83%
Moderate Homology
Sheep
83%
Moderate Homology
Zebrafish
37%
Low homology
Drosophila
10%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NKX2-5 antibody
Publications
Application:
Species:
Second heart field and the development of the outflow tract in human embryonic heart
Published: 2013-03-12
Journal: DEVELOPMENT GROWTH & DIFFERENTIATION
Application: IHC
Impact Factor: 2.40
Species: Human
PMID: 23488909
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Product Promise
We promise Anti-Nkx2.5/NKX2-5 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9293
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026