Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat. Cited in 6 publication(s).
Anti-HSP60/HSPD1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. Unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All SUMO proteins share the conserved ubiquitin domain and the C-terminal diglycine cleavage/attachment site. Human SUMO2, also known as Sentrin2 and SMT3B is synthesized as a 95 amino acid (aa), 11 kDa propeptide that contains a two aa C-terminal prosegment, and an 18 aa N-terminal protein interacting region (aa 33 -50). Following prosegment cleavage, the C-terminal glycine is enzymatically attached to a lysine on a target protein. Human SUMO2 shares 100% sequence identity to SUMO-2 from mouse. SUMO2 also has very high sequence homology to SUMO3 and SUMO4, 86 % and 85%, respectively. SUMO2 shares only 44% sequence identity to SUMO1.
|Product Name||Anti-HSP60/HSPD1 Antibody
See all HSP60 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for HSP60/HSPD1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. HSP60/HSPD1 information: Molecular Weight: 60955 MW; Subcellular Localization: Mitochondrion matrix .|
|Cite This Product||Anti-HSP60/HSPD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1106)|
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|Anti-HSP60/HSPD1 Antibody may replace the following items: sc 18771|sc 33558|sc 18767|sc 65262|sc 18766.|
|Reactivity/Species||Human, Mouse, Rat|
|Predicted Reactivity||Horse, Monkey, Rabbit
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60(503-516aa YDAMLGDFVNMVEK), different from the related human sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Hspd1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||63868|
|Species Of This Entry||Rat|
|Protein Name||60 kDa heat shock protein, mitochondrial|
|Superfamily||chaperonin (HSP60) family|
|Alternative Names||HSP60|60 kDa chaperonin; Chaperonin 60; cpn60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP60; HSP-60; HSP60SPG13; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; spastic paraplegia 13 (autosomal dominant); SPG13|
|Post Tranlational Modifications||Phosphorylation; Biotinylation; Glycosylation; Cleavage; Acetylation; Oxidation; Methylation|
|Gene Location||9q31, on Chromasome 9, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Hspd1 (Source: Uniprot.org, NCBI)
|Protein Function||Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.|
|Research Areas||Apoptosis; Cellular Markers; Loading Controls; Membrane Trafficking and Chaperones; Mitochondrial Markers|
|Related Diseases||Neoplasms; Malignant Neoplasms; Paraplegia; Spastic Paraplegia; Spastic Paraplegia, Hereditary; Inflammation; Neurodegenerative Disorders; Hyperplasia; Carcinoma; Nerve Degeneration; Leukodystrophy; Pelizaeus-merzbacher Disease; Diabetes Mellitus; Chlamydia Infections; Ovarian Neoplasm; Malignant Paraganglionic Neoplasm; Weakness; Malignant Neoplasm Of Lung|
|Related Pathways||Pathogenesis; Cell Cycle; Cell Proliferation; Spermatogenesis; Protein Folding; Transport; Fertilization; Immune Response; Cell Death; Cell Growth; Exocytosis; Regeneration; Acrosome Reaction; Reverse Transcription; Localization; Methylation; Rna Interference; Cell Adhesion; Chondrocyte Differentiation; Cell Division|
|Background||HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.|
|Scientific References||PMID: 1979858 by Peralta D., et al. cDNA and deduced amino acid sequence of rat liver prehsp60 (chaperonin-60).|
PMID: 1976241 by Venner T.J., et al. Nucleotide sequence of rat hsp60 (chaperonin, GroEL homolog) cDNA.
Our Boster Quality Guarantee for Anti-HSP60/HSPD1 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: CEM Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: COLO320 Cell Lysate
IHC(P): Rat Intestine Tissue
ICC: SMMC Cell
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 6
|Li L, Zhai S, Wang L, Si S, Wu H, Chang Z. Fish Shellfish Immunol. 2014 Feb;36(2):401-8. Doi: 10.1016/J.Fsi.2013.12.016. Epub 2013 Dec 29. Hsp60 In Caudal Fin Regeneration From Paramisgurnus Dabryanus: Molecular Cloning And Expression Characteriza...||PubMed ID 24380831|
|Li L, Nan P, Zhai S, Wang L, Si S, Chang Z. Mol Cell Biochem. 2014 Feb;387(1-2):143-50. Doi: 10.1007/S11010-013-1879-0. Epub 2013 Nov 21. Molecular Cloning, Characterization, And Expression Of Hsp60 In Caudal Fin Regeneration Of Misgurnus Anguilli...||PubMed ID 24257806|
|Hager L, Li L, Pun H, Liu L, Hossain Ma, Maguire Gf, Naples M, Baker C, Magomedova L, Tam J, Adeli K, Cummins Cl, Connelly Pw, Ng Ds. J Biol Chem. 2012 Jun 8;287(24):20755-68. Doi: 10.1074/Jbc.M112.340919. Epub 2012 Apr 12. Lecithin:Cholesterol Ac...||PubMed ID 22500017|
|Cui Yd, Du Yz, Lu Mx, Qiang Ck. J Insect Sci. 2010;10:100. Doi: 10.1673/031.010.10001. Cloning Of The Heat Shock Protein 60 Gene From The Stem Borer, Chilo Suppressalis, And Analysis Of Expression Characteristics Under Heat Stress.||PubMed ID 20673188|
|Hepatic mitochondrial and ER stress induced by defective PPAR? signaling in the pathogenesis of hepatic steatosis||PubMed ID 24735884|
|Expression and location of HSP60 and HSP10 in the heart tissue of heat-stressed rats||PubMed ID 27698781|
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