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Product Info Summary
| SKU: | RP1020 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MIF Antibody Picoband®
SKU/Catalog Number
RP1020
BA14437 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MIF Antibody catalog # RP1020. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MIF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1020)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MIF recombinant protein (Position: M1-A115).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1020 is reactive to MIF in Human
Observed Molecular Weight
12 kDa
Calculated molecular weight
12.5 kDa
Background of MIF
Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1kb. Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2. MIF plays a critical role in inflammatory diseases and atherogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1020 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Jurkat whole cell, human K562 whole cell, human 293T whole cell, human PC-3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of MIF using anti-MIF antibody (RP1020).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (Catalog # RP1020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.
Specific Publications For Anti-MIF Antibody Picoband® (RP1020)
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Customer Q&As
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4 Customer Q&As for Anti-MIF Antibody Picoband®
Question
We purchased anti-MIF antibody for ELISA on platelet last year. I am using human, and We intend to use the antibody for WB next. you antibody examining platelet as well as skin uterus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
I took a look at the website and datasheets of our anti-MIF antibody and it seems that RP1020 has been tested on human in both ELISA and WB. Thus RP1020 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-13
Question
We are currently using anti-MIF antibody RP1020 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-MIF antibody (RP1020) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
My boss were happy with the WB result of your anti-MIF antibody. However we have seen positive staining in liver secreted using this antibody. Is that expected? Could you tell me where is MIF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
According to literature, liver does express MIF. Generally MIF expresses in secreted. Regarding which tissues have MIF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, Lung, Skin, and Uterus, Pubmed ID: 15489334
Lens, Pubmed ID: 7679497
Liver, Pubmed ID: 1286669, 24275569
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2018-03-02
Question
We have seen staining in human skin uterus. What should we do? Is anti-MIF antibody supposed to stain skin uterus positively?
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
According to literature skin uterus does express MIF. According to Uniprot.org, MIF is expressed in left adrenal gland, brain, brain, lung, skin uterus, platelet, liver, lens, among other tissues. Regarding which tissues have MIF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, Lung, Skin, and Uterus, Pubmed ID: 15489334
Lens, Pubmed ID: 7679497
Liver, Pubmed ID: 1286669, 24275569
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2017-12-04
