Anti-MIF Antibody

Boster Bio Anti-MIF Antibody catalog # RP1020. Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: RP1020
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-MIF Antibody

See all MIF products

SKU/Catalog Number

RP1020

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MIF Antibody catalog # RP1020. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MIF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1020)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived human MIF recombinant protein (Position: M1-A115).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1020 is reactive to MIF in Human

Applications

RP1020 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA , 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MIF (Source: Uniprot.Org, NCBI)

Uniprot ID

P14174

Gene ID

4282

Gene Name

MIF

Full Name

Macrophage migration inhibitory factor

Weight

12.476kDa

Superfamily

MIF family

Alternative Names

EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase MIF GIF, GLIF, MMIF macrophage migration inhibitory factor macrophage migration inhibitory factor|L-dopachrome isomerase|L-dopachrome tautomerase|epididymis secretory sperm binding protein|macrophage migration inhibitory factor (glycosylation-inhibiting factor)|phenylpyruvate tautomerase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MIF, check out the MIF Infographic

MIF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MIF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for RP1020

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4 Customer Q&As for Anti-MIF Antibody

Question

We purchased anti-MIF antibody for ELISA on platelet last year. I am using human, and We intend to use the antibody for WB next. you antibody examining platelet as well as skin uterus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

I took a look at the website and datasheets of our anti-MIF antibody and it seems that RP1020 has been tested on human in both ELISA and WB. Thus RP1020 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-13

Question

We are currently using anti-MIF antibody RP1020 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

The anti-MIF antibody (RP1020) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-11

Question

My boss were happy with the WB result of your anti-MIF antibody. However we have seen positive staining in liver secreted using this antibody. Is that expected? Could you tell me where is MIF supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-03-02

Answer

According to literature, liver does express MIF. Generally MIF expresses in secreted. Regarding which tissues have MIF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, Lung, Skin, and Uterus, Pubmed ID: 15489334
Lens, Pubmed ID: 7679497
Liver, Pubmed ID: 1286669, 24275569
Platelet, Pubmed ID: 12665801

Boster Scientific Support

Answered: 2018-03-02

Question

We have seen staining in human skin uterus. What should we do? Is anti-MIF antibody supposed to stain skin uterus positively?

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

According to literature skin uterus does express MIF. According to Uniprot.org, MIF is expressed in left adrenal gland, brain, brain, lung, skin uterus, platelet, liver, lens, among other tissues. Regarding which tissues have MIF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, Lung, Skin, and Uterus, Pubmed ID: 15489334
Lens, Pubmed ID: 7679497
Liver, Pubmed ID: 1286669, 24275569
Platelet, Pubmed ID: 12665801

Boster Scientific Support

Answered: 2017-12-04

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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RP1020
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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