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Product Info Summary
| SKU: | M01767 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Hu CD133 Purified PROM1 Monoclonal Antibody
SKU/Catalog Number
M01767
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (Catalog# M01767). Tested in Flow Cytometry, WB, IHC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01767)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
W6B3C1
Isotype
Mouse IgG1
Immunogen
WERI-RB-1 retinoblastoma cell line. The mouse monoclonal antibody W6B3C1 recognizes the extracellular glycosylated epitope 1 on human CD133 (CD133/1), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of stem cells and tumor cells.
Reactive Species
M01767 is reactive to PROM1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
97.2 kDa
Background of PROM1
CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01767 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Western blotting: 1-2 μg/ml; non-reducing conditions preferred.
Validation Images & Assay Conditions
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Flow cytometry analysis (surface staining) of human peripheral whole blood showing CD133 positive stem cells (blue) and lymphocytes (red) stained using anti-CD133 (W6B3C1) purified, GAM-FITC.
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Western blotting analysis of human CD133 using mouse monoclonal antibody W6B3C1 on lysates of HT29 cells and Jurkat cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD133 was detected around 125 kDa.
Specific Publications For Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (M01767)
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