Click image to see more details
Product Info Summary
| SKU: | M01508-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, ICC, WB, CyTOF |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD33 Purified Monoclonal Antibody
SKU/Catalog Number
M01508-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD33 Purified Monoclonal Antibody (Catalog# M01508-1). Tested in Flow Cytometry, IP, WB, IHC-F, ICC, MC (CyTOF) application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD33 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01508-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
WM53
Isotype
Mouse IgG1
Immunogen
Human AML cells. The mouse monoclonal antibody WM53 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Reactive Species
M01508-1 is reactive to CD33 in Human, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
39.8 kDa
Background of CD33
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01508-1 is guaranteed for Flow Cytometry, IP, IHC-F, ICC, WB, CyTOF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD33 (WM53) purified, GAM-APC.
Click image to see more details
Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.
Specific Publications For Anti-Hu CD33 Purified Monoclonal Antibody (M01508-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD33 Purified Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Hu CD33 Purified Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
