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Product Info Summary
| SKU: | M01022-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD35 Purified CR1 Monoclonal Antibody
SKU/Catalog Number
M01022-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD35 Purified CR1 Monoclonal Antibody (Catalog# M01022-1). Tested in Flow Cytometry, IP, WB, IHC-P, IHC-F, ICC application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD35 Purified CR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01022-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
E11
Isotype
Mouse IgG1
Immunogen
Acute monocytic leukemia cells and normal blood monocytes. The mouse monoclonal antibody E11 recognizes an extracellular epitope of CD35 (CR1), a type I transmembrane glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.
Reactive Species
M01022-1 is reactive to CR1 in Human, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
223.7 kDa
Background of CR1
CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01022-1 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval.
Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Separation of human CD35 positive lymphocytes (red-filled) from CD35 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD35 (E11) purified antibody (concentration in sample 3 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD35 (E11) purified antibody (concentration in sample 3 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD35 Purified CR1 Monoclonal Antibody (M01022-1)
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