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Product Info Summary
| SKU: | M03281-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, WB |
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Product info
Product Name
Anti-Hu CD48 Purified Monoclonal Antibody
SKU/Catalog Number
M03281-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD48 Purified Monoclonal Antibody (Catalog# M03281-1). Tested in Flow Cytometry, IP, WB, IHC-P application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD48 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03281-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-102
Isotype
Mouse IgG1
Immunogen
Raji human Burkitt's lymphoma cell line. The antibody MEM-102 reacts with CD48 (Blast-1), a 40-47 kDa GPI-anchored extracellular membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells; it is negative on granulocytes, platelets and erythrocytes.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03281-1 is reactive to CD48 in Human, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
27.7 kDa
Background of CD48
CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03281-1 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml.
Immunohistochemistry: 5-10 μg/ml.
Validation Images & Assay Conditions
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Separation of CD48 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD48 Purified Monoclonal Antibody (M03281-1)
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