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Product Info Summary
| SKU: | M00652-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD62L Purified SELL Monoclonal Antibody
SKU/Catalog Number
M00652-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD62L Purified SELL Monoclonal Antibody (Catalog# M00652-2). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD62L Purified SELL Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00652-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
DREG56
Isotype
Mouse IgG1
Immunogen
PMA-activated human peripheral blood leukocytes. The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
Reactive Species
M00652-2 is reactive to SELL in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.2 kDa
Background of SELL
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00652-2 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml.
Western blotting: Non-reducing conditions.
Validation Images & Assay Conditions
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Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD62L Purified SELL Monoclonal Antibody (M00652-2)
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