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Product Info Summary
| SKU: | M01047-4 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Chicken, Cow, Dog, Guinea pig, Human, Mouse, Pig, Rabbit, Rat, Primate, Horse |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, WB |
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Product info
Product Name
Anti-Hu CD79a Purified Monoclonal Antibody
SKU/Catalog Number
M01047-4
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD79a Purified Monoclonal Antibody (Catalog# M01047-4). Tested in Flow Cytometry, IP, WB, IHC-P application(s). This antibody reacts with Dog, Chicken, Rabbit, Guinea pig, Horse, Cow, Mouse, Rat, Pig, Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD79a Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01047-4)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
HM47
Isotype
Mouse IgG1 kappa
Immunogen
Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a. The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
Reactive Species
M01047-4 is reactive to CD79A in Chicken, Cow, Dog, Guinea pig, Human, Mouse, Pig, Rabbit, Rat, Primate, Horse
Observed Molecular Weight
42 kDa
Calculated molecular weight
25.0 kDa
Background of CD79A
CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01047-4 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml, intracellular staining.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Separation of human CD79a positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD79a (HM47) purified antibody (concentration in sample 4 µg/ml, GAM APC).
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Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human CD79a (HM47) purified antibody (concentration in sample 4 µg/ml, GAM APC).
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Western blotting analysis of human CD79a using mouse monoclonal antibody HM47 on lysates of Raji and Jurkat (negative control) cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD79a monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD79a was detected around 43 kDa.
Specific Publications For Anti-Hu CD79a Purified Monoclonal Antibody (M01047-4)
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