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Product Info Summary
| SKU: | A00412-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-PRDM1 BLIMP1 Antibody
SKU/Catalog Number
A00412-2
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-PRDM1 BLIMP1 Antibody (Catalog # A00412-2). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store Blimp1 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-PRDM1 BLIMP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00412-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Blimp1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00412-2 is reactive to PRDM1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
95.8 kDa
Background of PRDM1
Anti-Blimp1 is ideal for Immunology, Epigenetic and Nuclear Signaling research. PRDM1/Blimp1 (PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Positive regulatory domain I-binding factor 1, PRDI-binding factor 1, or PRDI-BF1, or Beta-lymphocyte-induced maturation protein 1) is one of several transcription factors which drives the maturation of B-lymphocytes into Ig secreting cells. In the nucleus, PRDM1/BLIMP1 functions as a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Transcription of the PRDM1 gene is increased upon virus induction. PRDM1/Blimp1 also interacts with PRMT5. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00412-2 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:40,000
IHC: 5µg/mL
IP: 5-10 µg/mL
WB: 1:1,000
This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~88 kDa in size corresponding to BLIMP1 by western blotting in the appropriate cell lysate or extract.
Validation Images & Assay Conditions
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Western blots using Boster's affinity purified anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 at ~88kDa. Lane 1: mock Raji cell lysate. Lane 2: overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate. Lane 3: Raji whole cell nuclear extract. Lane 4: endogenous PRDM1/BLIMP1 in human plasma cell nuclear extract. The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween. Personal communication, Hyesuk Yoon and Jerry Boss, Emory University, Atlanta, GA.
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Immunohistochemistry of rabbit anti-PRDM1-BLMP1 antibody. Tissue: colon. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PRDM1-BLMP1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PRDM1-BLMP1 as precipitated red signal with hematoxylin purple nuclear counterstain.
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Immunohistochemistry of rabbit anti-PRDM1-BLMP1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PRDM1-BLMP1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PRDM1-BLMP1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Specific Publications For Anti-PRDM1 BLIMP1 Antibody (A00412-2)
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