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Product Info Summary
| SKU: | M01394-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IBA1 AIF1 Monoclonal Antibody
SKU/Catalog Number
M01394-1
BM5765 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IBA1 AIF1 Monoclonal Antibody catalog # M01394-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IBA1 AIF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01394-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFGH-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IBA1
Reactive Species
M01394-1 is reactive to AIF1 in Human
Observed Molecular Weight
17 kDa
Calculated molecular weight
16.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01394-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:100
Positive Control
WB human THP-1 whole cell, human U937 whole cell, human HL-60 whole cell, human HEL whole cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Western blot analysis of IBA1 using anti-IBA1 antibody (M01394-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human U937 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IBA1 antigen affinity purified monoclonal antibody (M01394-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IBA1 at approximately 17 kDa. The expected band size for IBA1 is at 17 kDa.
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IHC analysis of IBA1 using anti-IBA1 antibody (M01394-1).
IBA1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IBA1 Antibody (M01394-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-IBA1 AIF1 Monoclonal Antibody (M01394-1)
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Customer Q&As
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16 Customer Q&As for Anti-IBA1 AIF1 Monoclonal Antibody
Question
I was wanting to use your anti-IBA1 Monoclonal antibody for IHC for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
As indicated on the product datasheet, M01394-1 anti-IBA1 Monoclonal antibody has been validated for IF, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-07
Question
I have a question about product M01394-1, anti-IBA1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01394-1 anti-IBA1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-06
Question
I am looking for to test anti-IBA1 Monoclonal antibody M01394-1 on mouse liver for research purposes, then I may be interested in using anti-IBA1 Monoclonal antibody M01394-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
The products we sell, including anti-IBA1 Monoclonal antibody M01394-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-09
Question
I see that the anti-IBA1 Monoclonal antibody M01394-1 works with IHC, what is the protocol used to produce the result images on the product page?
C. Walker
Verified customer
Asked: 2020-03-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-02
Question
Would M01394-1 be suitable in methanol fixed IHC stainings of mouse brain cryosections?
Verified customer
Asked: 2020-02-15
Answer
The Anti-IBA1 AIF1 Monoclonal Antibody (M01394-1) is not suitable for working in methanol fixed IHC stainings of mouse brain cryosections. We recommend M01394 for this specific use.
Boster Scientific Support
Answered: 2020-02-16
Question
Is there a BSA free version of anti-IBA1 Monoclonal antibody M01394-1 available?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IBA1 Monoclonal antibody M01394-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-30
Question
We have been able to see staining in rat liver. Are there any suggestions? Is anti-IBA1 Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
From literature liver does express AIF1. From Uniprot.org, AIF1 is expressed in metanephric glomerulus, lymphocyte, glial tumor, prostate, erythroleukemia, liver, among other tissues. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-14
Question
We are currently using anti-IBA1 Monoclonal antibody M01394-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The anti-IBA1 Monoclonal antibody (M01394-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-04
Question
Is this M01394-1 anti-IBA1 Monoclonal antibody reactive to the isotypes of AIF1?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The immunogen of M01394-1 anti-IBA1 Monoclonal antibody is A synthesized peptide derived from human IBA1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-06
Question
Here is the WB image, lot number and protocol we used for liver using anti-IBA1 Monoclonal antibody M01394-1. Please let me know if you require anything else.
N. Parker
Verified customer
Asked: 2019-04-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-19
Question
We purchased anti-IBA1 Monoclonal antibody for IHC on metanephric glomerulus last year. I am using rat, and We are going to use the antibody for IF next. I am interested in examining metanephric glomerulus as well as lymphocyte in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
I have checked the website and datasheets of our anti-IBA1 Monoclonal antibody and I see that M01394-1 has been validated on rat in both IHC and IF. Thus M01394-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-11-06
Question
Is a blocking peptide available for product anti-IBA1 Monoclonal antibody (M01394-1)?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
We do provide the blocking peptide for product anti-IBA1 Monoclonal antibody (M01394-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-24
Question
My team were well pleased with the WB result of your anti-IBA1 Monoclonal antibody. However we have observed positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is AIF1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
Based on literature, liver does express AIF1. Generally AIF1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-07-11
Question
Would anti-IBA1 Monoclonal antibody M01394-1 work for IHC with liver?
M. Zhang
Verified customer
Asked: 2017-02-07
Answer
According to the expression profile of liver, AIF1 is highly expressed in liver. So, it is likely that anti-IBA1 Monoclonal antibody M01394-1 will work for IHC with liver.
Boster Scientific Support
Answered: 2017-02-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-IBA1 Monoclonal antibody M01394-1. Let me know if you need anything else.
K. Jackson
Verified customer
Asked: 2016-03-02
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-02
Question
Will M01394-1 anti-IBA1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Banerjee
Verified customer
Asked: 2015-06-18
Answer
As indicated on the product datasheet, M01394-1 anti-IBA1 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-06-18
