Anti-IBA1 AIF1 Monoclonal Antibody

Boster Bio Anti-IBA1 AIF1 Monoclonal Antibody catalog # M01394-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 8 publication(s).

Product Info Summary

SKU: M01394-1
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-IBA1 AIF1 Monoclonal Antibody

See all AIF-1/Iba1 products

SKU/Catalog Number

M01394-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-IBA1 AIF1 Monoclonal Antibody catalog # M01394-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IBA1 AIF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01394-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFGH-1

Immunogen

A synthesized peptide derived from human IBA1

Reactive Species

M01394-1 is reactive to AIF1 in Human

Applications

M01394-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500~1:2000
IHC 1:50~1:200
ICC/IF 1:50~1:200
Flow Cytometry 1:100

Validation Images & Assay Conditions

Gene/Protein Information For AIF1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P55008

Gene ID

199

Gene Name

AIF1

Full Name

Allograft inflammatory factor 1

Weight

16.703kDa

Alternative Names

AIF1; AIF-1; AIF-1IRT1; allograft inflammatory factor 1; G1; IBA1; IBA1Em:AF129756.17; IbaI; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT1; IRT-1; Protein G1 AIF1 AIF-1, IBA1, IRT-1, IRT1 allograft inflammatory factor 1 allograft inflammatory factor 1|interferon gamma responsive transcript|ionized calcium-binding adapter molecule 1|protein G1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AIF1, check out the AIF1 Infographic

AIF1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AIF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01394-1 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M01394-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:1000

Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
M01394-1 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:500

Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
M01394-1 usage in article: APP:IHC, SAMPLE:BRAIN SECTION, DILUTION:1:400

Mohamed MY,Masocha W. Indomethacin augments lipopolysaccharide-induced expression of inflammatory molecules in the mouse brain. PeerJ.2020 Nov 18;8:e10391.doi:10.7717/peerj.10391.PMID:33240677;PMCID:PMC7680052.
Species: Mouse
M01394-1 usage in article: APP:WB, SAMPLE:BRAIN HOMOGENATES, DILUTION:1:50

Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture

Toll-like receptor 4 contributes to chronic itch, alloknesis, and spinal astrocyte activation in male mice

Re-infection of the prion from the scrapie?infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

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16 Customer Q&As for Anti-IBA1 AIF1 Monoclonal Antibody

Question

I was wanting to use your anti-IBA1 Monoclonal antibody for IHC for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

As indicated on the product datasheet, M01394-1 anti-IBA1 Monoclonal antibody has been validated for IF, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-05-07

Question

I have a question about product M01394-1, anti-IBA1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01394-1 anti-IBA1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-05-06

Question

I am looking for to test anti-IBA1 Monoclonal antibody M01394-1 on mouse liver for research purposes, then I may be interested in using anti-IBA1 Monoclonal antibody M01394-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-09

Answer

The products we sell, including anti-IBA1 Monoclonal antibody M01394-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-09

Question

I see that the anti-IBA1 Monoclonal antibody M01394-1 works with IHC, what is the protocol used to produce the result images on the product page?

C. Walker

Verified customer

Asked: 2020-03-02

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-02

Question

Would M01394-1 be suitable in methanol fixed IHC stainings of mouse brain cryosections?

Verified customer

Asked: 2020-02-15

Answer

The Anti-IBA1 AIF1 Monoclonal Antibody (M01394-1) is not suitable for working in methanol fixed IHC stainings of mouse brain cryosections. We recommend M01394 for this specific use.

Boster Scientific Support

Answered: 2020-02-16

Question

Is there a BSA free version of anti-IBA1 Monoclonal antibody M01394-1 available?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IBA1 Monoclonal antibody M01394-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-30

Question

We have been able to see staining in rat liver. Are there any suggestions? Is anti-IBA1 Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

From literature liver does express AIF1. From Uniprot.org, AIF1 is expressed in metanephric glomerulus, lymphocyte, glial tumor, prostate, erythroleukemia, liver, among other tissues. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-10-14

Question

We are currently using anti-IBA1 Monoclonal antibody M01394-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

The anti-IBA1 Monoclonal antibody (M01394-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-04

Question

Is this M01394-1 anti-IBA1 Monoclonal antibody reactive to the isotypes of AIF1?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

The immunogen of M01394-1 anti-IBA1 Monoclonal antibody is A synthesized peptide derived from human IBA1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-06

Question

Here is the WB image, lot number and protocol we used for liver using anti-IBA1 Monoclonal antibody M01394-1. Please let me know if you require anything else.

N. Parker

Verified customer

Asked: 2019-04-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-19

Question

We purchased anti-IBA1 Monoclonal antibody for IHC on metanephric glomerulus last year. I am using rat, and We are going to use the antibody for IF next. I am interested in examining metanephric glomerulus as well as lymphocyte in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2018-11-06

Answer

I have checked the website and datasheets of our anti-IBA1 Monoclonal antibody and I see that M01394-1 has been validated on rat in both IHC and IF. Thus M01394-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-11-06

Question

Is a blocking peptide available for product anti-IBA1 Monoclonal antibody (M01394-1)?

Verified Customer

Verified customer

Asked: 2018-05-24

Answer

We do provide the blocking peptide for product anti-IBA1 Monoclonal antibody (M01394-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-05-24

Question

My team were well pleased with the WB result of your anti-IBA1 Monoclonal antibody. However we have observed positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is AIF1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-07-11

Answer

Based on literature, liver does express AIF1. Generally AIF1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-07-11

Question

Would anti-IBA1 Monoclonal antibody M01394-1 work for IHC with liver?

M. Zhang

Verified customer

Asked: 2017-02-07

Answer

According to the expression profile of liver, AIF1 is highly expressed in liver. So, it is likely that anti-IBA1 Monoclonal antibody M01394-1 will work for IHC with liver.

Boster Scientific Support

Answered: 2017-02-07

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-IBA1 Monoclonal antibody M01394-1. Let me know if you need anything else.

K. Jackson

Verified customer

Asked: 2016-03-02

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-03-02

Question

Will M01394-1 anti-IBA1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Banerjee

Verified customer

Asked: 2015-06-18

Answer

As indicated on the product datasheet, M01394-1 anti-IBA1 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-06-18

Size

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M01394-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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