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- Table of Contents
AIF1 antibody (M01394-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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IHC analysis of IBA1 using anti-IBA1 antibody (M01394-1).
IBA1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IBA1 Antibody (M01394-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
IBA1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IBA1 Antibody (M01394-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-IBA1 AIF1 Monoclonal Antibody
AIF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
AIF1 antibody was positively detected by IHC in:
human tonsil tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:100
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
FC
Human
Recommend
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-IBA1 AIF1 Monoclonal Antibody
View all AIF1 Antibodies
View all AIF1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFGH-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IBA1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated Molecular Weight
16.7 kDa
Cite This Product
Anti-IBA1 AIF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01394-1)
Protocols
Immunohistochemistry (IHC) protocol for AIF1 antibody
Publications
Application:
Species:
METTL3 modulates bone Cancer pain via m6A-dependent JAK2/STAT3 activation in spinal astrocytes
Published: 2025-08-03
Journal: CELLULAR SIGNALLING
Application: FC
Impact Factor: 3.70
Species: Mouse
PMID: 40752537
An artificial protein modulator reprogramming neuronal protein functions
Published: 2024-03-06
Journal: Nature Communications
Application: IHC
Impact Factor: 14.70
Species: Mouse
PMID: 38448420
Indomethacin augments lipopolysaccharide-induced expression of inflammatory molecules in the mouse brain
Published: 2020-11-18
Journal: PeerJ
Application: WB
Impact Factor: 2.38
Species: Mouse
PMID: 33240677
Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery
Published: 2020-09-11
Journal: ADVANCED FUNCTIONAL MATERIALS
Application: IF
Impact Factor: 16.84
Species: Mouse
MicroRNA134 of Ventral Hippocampus Is Involved in Cocaine Extinction-Induced Anxiety-like and Depression-like Behaviors in Mice
Published: 2020-01-10
Journal: Molecular Therapy-Nucleic Acids
Application: WB
Impact Factor: 7.03
Species: Mouse
PMID: 32004865
Chronic periodontitis induces microbiota-gut-brain axis disorders and cognitive impairment in mice
Published: 2020-01-08
Journal: EXPERIMENTAL NEUROLOGY
Application: IF
Impact Factor: 4.69
Species: Mouse
PMID: 31926167
β-Caryophyllene, a CB2-Receptor-Selective Phytocannabinoid, Suppresses Mechanical Allodynia in a Mouse Model of Antiretroviral-Induced Neuropathic Pain
Published: 2019-12-27
Journal: MOLECULES
Application: WB
Impact Factor: 3.06
Species: Mouse
PMID: 31892132
Treatment with MQA, a Derivative of Caffeoylquinic Acid, Provides Neuroprotective Effects against Cerebral Ischemia Through Suppression of the p38 Pathway and Oxidative Stress in Rats
Published: 2019-02-08
Journal: JOURNAL OF MOLECULAR NEUROSCIENCE
Application: IHC
Impact Factor: 2.58
Species: Rat
PMID: 30734208
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Product Promise
We promise Anti-IBA1 AIF1 Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01394-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026