Anti-IGF1 Receptor/IGF1R Antibody

Boster Bio Anti-IGF1 Receptor/IGF1R Antibody catalog # PA1746. Tested in IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1746
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC-P, ICC, WB

Product Name

Anti-IGF1 Receptor/IGF1R Antibody

See all IGF-I R/IGF1R products

SKU/Catalog Number

PA1746

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IGF1 Receptor/IGF1R Antibody catalog # PA1746. Tested in IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IGF1 Receptor/IGF1R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1746)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor (1353-1367aa RKNERALPLPQSSTC), different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1746 is reactive to IGF1R in Human, Mouse, Rat

Applications

PA1746 is guaranteed for IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IGF1R (Source: Uniprot.Org, NCBI)

Uniprot ID

P08069

Gene ID

3480

Gene Name

IGF1R

Full Name

Insulin-like growth factor 1 receptor

Weight

154.793kDa

Superfamily

protein kinase superfamily

Alternative Names

CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2 IGF1R CD221, IGFIR, IGFR, JTK13 insulin like growth factor 1 receptor insulin-like growth factor 1 receptor|IGF-I receptor|soluble IGF1R variant 1|soluble IGF1R variant 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IGF1R, check out the IGF1R Infographic

IGF1R infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IGF1R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1746 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of IGF-1R, VEGF-C and D2-40 and their correlation with lymph node metastasis in endometrial adenocarcinoma

Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway

Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

IGF-1/IGF-1R/hsa-let-7c axis regulates the committed differentiation of stem cells from apical papilla

Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression

Liu GX, Ma S, Li Y, Yu Y, Zhou YX, Lu YD, Jin L, Wang ZL, Yu JH. Exp Mol Med. 2018 Apr 13;50(4):25. doi: 10.1038/s12276-018-0048-7. Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps...

Lai J, Chen F, Chen J, Ruan G, He M, Chen C, Tang J, Wang DW. Sci Rep. 2017 Mar 14;7:44473. doi: 10.1038/srep44473. Overexpression of decorin promoted angiogenesis in diabetic cardiomyopathy via IGF1R-AKT-VEGF signaling

Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010

Ramdhave AS, Ojha S, Nandave M. Am J Transl Res. 2017 Mar 15;9(3):830-844. eCollection 2017. Energy intake correlates with the levels of fatty acid synthase and insulin-like growth factor-1 in male and female C57BL/6 mice.

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19 Customer Q&As for Anti-IGF1 Receptor/IGF1R Antibody

Question

We want using your anti-IGF1 Receptor/IGF1R antibody for glucose homeostasis studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

We appreciate your inquiry. This PA1746 anti-IGF1 Receptor/IGF1R antibody is tested on 293t cell lysate, a549 cell lysate, rat pancreas tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-13

Question

We want to test anti-IGF1 Receptor/IGF1R antibody PA1746 on rat fetal brain for research purposes, then I may be interested in using anti-IGF1 Receptor/IGF1R antibody PA1746 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

The products we sell, including anti-IGF1 Receptor/IGF1R antibody PA1746, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-29

Question

Will anti-IGF1 Receptor/IGF1R antibody PA1746 work for WB with fetal brain?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

According to the expression profile of fetal brain, IGF1R is highly expressed in fetal brain. So, it is likely that anti-IGF1 Receptor/IGF1R antibody PA1746 will work for WB with fetal brain.

Boster Scientific Support

Answered: 2020-01-13

Question

Does anti-IGF1 Receptor/IGF1R antibody PA1746 work on zebrafish WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

Our lab technicians have not validated anti-IGF1 Receptor/IGF1R antibody PA1746 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IGF1 Receptor/IGF1R antibody PA1746 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-07

Question

Here is the WB image, lot number and protocol we used for fetal brain using anti-IGF1 Receptor/IGF1R antibody PA1746. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-28

Question

We are currently using anti-IGF1 Receptor/IGF1R antibody PA1746 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

The anti-IGF1 Receptor/IGF1R antibody (PA1746) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-21

Question

Will PA1746 anti-IGF1 Receptor/IGF1R antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

You can see on the product datasheet, PA1746 anti-IGF1 Receptor/IGF1R antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-20

Question

I have a question about product PA1746, anti-IGF1 Receptor/IGF1R antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1746 anti-IGF1 Receptor/IGF1R antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-24

Question

Do you have a BSA free version of anti-IGF1 Receptor/IGF1R antibody PA1746 available?

T. Huang

Verified customer

Asked: 2019-04-09

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IGF1 Receptor/IGF1R antibody PA1746 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-09

Question

I was wanting to use your anti-IGF1 Receptor/IGF1R antibody for WB for rat fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fetal brain identification?

Verified Customer

Verified customer

Asked: 2019-01-02

Answer

It shows on the product datasheet, PA1746 anti-IGF1 Receptor/IGF1R antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-02

Question

Is a blocking peptide available for product anti-IGF1 Receptor/IGF1R antibody (PA1746)?

Verified Customer

Verified customer

Asked: 2018-10-30

Answer

We do provide the blocking peptide for product anti-IGF1 Receptor/IGF1R antibody (PA1746). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-10-30

Question

Will PA1746 cross-react with other human receptors like IRS Insulin Receptor Substrates?

Verified Customer

Verified customer

Asked: 2018-10-25

Answer

PA1746 does not react with IRS1, we do not have other relevant protein to verify the cross reactivity.

Boster Scientific Support

Answered: 2018-10-25

Question

We have been able to see staining in rat melanocyte. Are there any suggestions? Is anti-IGF1 Receptor/IGF1R antibody supposed to stain melanocyte positively?

H. Baker

Verified customer

Asked: 2018-08-17

Answer

Based on literature melanocyte does express IGF1R. Based on Uniprot.org, IGF1R is expressed in caput epididymis, placenta, fetal brain, melanocyte, cervix carcinoma, among other tissues. Regarding which tissues have IGF1R expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Fetal brain, Pubmed ID: 16572171
Melanocyte, Pubmed ID: 8247543, 1846292
Placenta, Pubmed ID: 2877871, 8257688

Boster Scientific Support

Answered: 2018-08-17

Question

My team were well pleased with the WB result of your anti-IGF1 Receptor/IGF1R antibody. However we have seen positive staining in melanocyte cell membrane using this antibody. Is that expected? Could you tell me where is IGF1R supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-08-02

Answer

Based on literature, melanocyte does express IGF1R. Generally IGF1R expresses in cell membrane. Regarding which tissues have IGF1R expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Fetal brain, Pubmed ID: 16572171
Melanocyte, Pubmed ID: 8247543, 1846292
Placenta, Pubmed ID: 2877871, 8257688

Boster Scientific Support

Answered: 2018-08-02

Question

We bought anti-IGF1 Receptor/IGF1R antibody for WB on caput epididymis a few months ago. I am using rat, and We want to use the antibody for IHC next. Our lab want to know about examining caput epididymis as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2017-05-11

Answer

I looked at the website and datasheets of our anti-IGF1 Receptor/IGF1R antibody and it seems that PA1746 has been tested on rat in both WB and IHC. Thus PA1746 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-05-11

Question

I see that the anti-IGF1 Receptor/IGF1R antibody PA1746 works with WB, what is the protocol used to produce the result images on the product page?

N. Patel

Verified customer

Asked: 2014-10-29

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-10-29

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-IGF1 Receptor/IGF1R antibody PA1746. Let me know if you need anything else.

S. Mitchell

Verified customer

Asked: 2014-10-08

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-08

Question

A client of ours is interested in your PA1746 IGF1R Antibody, he needs to know if this IGF1 Human Receptor cross-react with other human receptors like IRS Insulin Receptor Substrates. Did you ever make any specific tests on this concern? Can you indicate if there is publication regarding this cross-reaction?

Verified Customer

Verified customer

Asked: 2014-04-28

Answer

Test result shows that PA1746 does not react with IRS1. This is what we can do right now. We haven't got more relevant protein to verify the cross reactivity. PA2269 IRS1 ac3 PB9223 IRS1 BP71 PB9224 IRS2 BP69

Boster Scientific Support

Answered: 2014-04-28

Question

Is this PA1746 anti-IGF1 Receptor/IGF1R antibody reactive to the isotypes of IGF1R?

S. Bhatt

Verified customer

Asked: 2013-09-25

Answer

The immunogen of PA1746 anti-IGF1 Receptor/IGF1R antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor(1353-1367aa RKNERALPLPQSSTC), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-09-25

Size

Total: $280

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PA1746
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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