Anti-IGF1 Receptor/IGF1R Antibody
Rabbit IgG polyclonal antibody for Insulin-like growth factor 1 receptor(IGF1R) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 10 publication(s).
Anti-IGF1 Receptor/IGF1R Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-IGF1 Receptor/IGF1R Antibody
See all IGF-I R/IGF1R primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for IGF1 RECEPTOR/IGF1R detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. IGF1 RECEPTOR/IGF1R information: Molecular Weight: 154793 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein ; Tissue Specificity: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.|
|Cite This Product||Anti-IGF1 Receptor/IGF1R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1746)|
|Similar Products From
|Anti-IGF1 Receptor/IGF1R Antibody may replace the following items: sc 101703|sc 101704|sc 135766|sc 135767|sc 25103|sc 25103 R|sc 271606|sc 293102|sc 31361|sc 31362|sc 390130|sc 398250|sc 461|sc 462|sc 463|sc 712|sc 713|sc 7952|sc 80985|sc 80986|sc 80987|sc 81167|sc 81464|sc 81499|sc 81764|sc 9038.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor(1353-1367aa RKNERALPLPQSSTC), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For IGF1R (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3480|
|Species Of This Entry||Human|
|Protein Name||Insulin-like growth factor 1 receptor|
|Superfamily||protein kinase superfamily|
|Alternative Names||IGF-I R/IGF1R|CD221 antigen; CD221; EC 2.7.10; EC 188.8.131.52; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2|
|Post Tranlational Modifications||Phosphorylation; Methylation; Cleavage; Demethylation; Oxidation; Ubiquitination; Glycosylation; Acetylation|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Gene Location||15q26.3, on Chromasome 15, gene sequence: NC_000015.10 (98648539..98964530)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IGF1R (Source: Uniprot.org, NCBI)
|Protein Function||Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K- driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.|
|Tissue Specificity||Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.|
|Interacting Proteins||ARHGEF12; CAMP; Crk; CSK; CTNNB1; IGF1; KCNIP3; LEF1; MDM2; PIK3R1; PIK3R3; PPM1A; PTPN1; PTPN11; PTPN6; TAGLN|
|Related Diseases||Malignant Neoplasms; Neoplasms; Malignant Neoplasm Of Breast; Mammary Neoplasms; Neoplasm Metastasis; Carcinoma; Growth Retardation; Malignant Paraganglionic Neoplasm; Cell Transformation, Neoplastic; Fetal Growth Retardation; Dwarfism; Diabetes Mellitus; Insulin Resistance; Growth Disorders; Adenocarcinoma; Hypertrophy; Cell Invasion; Lung Neoplasms|
|Related Pathways||Cell Proliferation; Cell Growth; Pathogenesis; Aging; Cell Cycle; Cell Death; Secretion; Localization; Gene Silencing; Angiogenesis; Cell Migration; Rna Interference; Methylation; Translation; Insulin Secretion; Cell Differentiation; Dna Methylation; Endocytosis; Glycolysis; Transport|
|Background||IGF1R(Insulin-like Growth Factor 1(IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1(IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, Dey et al.(1998) identified a regulatory subunit of phosphatidylinositol(PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.|
|Scientific References||PMID: 2877871 by Ullrich A., et al. Insulin-like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity.|
PMID: 1316909 by Abbot A.M., et al. Insulin-like growth factor I receptor gene structure.
Our Boster Quality Guarantee for Anti-IGF1 Receptor/IGF1R Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: 293T Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: MCF-7 Cell Lysate
IHC(P): Rat Pancreas Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 10
|Ramdhave AS, Ojha S, Nandave M. Am J Transl Res. 2017 Mar 15;9(3):830-844. eCollection 2017. Energy intake correlates with the levels of fatty acid synthase and insulin-like growth factor-1 in male and female C57BL/6 mice.||PubMed ID 28386316|
|Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010||PubMed ID 28669683|
|Lai J, Chen F, Chen J, Ruan G, He M, Chen C, Tang J, Wang DW. Sci Rep. 2017 Mar 14;7:44473. doi: 10.1038/srep44473. Overexpression of decorin promoted angiogenesis in diabetic cardiomyopathy via IGF1R-AKT-VEGF signaling||PubMed ID 28290552|
|Liu GX, Ma S, Li Y, Yu Y, Zhou YX, Lu YD, Jin L, Wang ZL, Yu JH. Exp Mol Med. 2018 Apr 13;50(4):25. doi: 10.1038/s12276-018-0048-7. Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps...||PubMed ID 29650947|
|Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression||PubMed ID 27725848|
|IGF-1/IGF-1R/hsa-let-7c axis regulates the committed differentiation of stem cells from apical papilla||PubMed ID 27833148|
|Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome||PubMed ID 19424673|
|Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression||PubMed ID 22720981|
|Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway||PubMed ID 27340351|
|Expression of IGF-1R, VEGF-C and D2-40 and their correlation with lymph node metastasis in endometrial adenocarcinoma||PubMed ID 22335030|
Order Product (PA1746)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.