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Product Info Summary
| SKU: | PA1746 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IGF1 Receptor/IGF1R Antibody Picoband®
SKU/Catalog Number
PA1746
BA0498 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IGF1 Receptor/IGF1R Antibody catalog # PA1746. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGF1 Receptor/IGF1R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1746)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor, different from the related rat and mouse sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1746 is reactive to IGF1R in Human, Mouse, Rat
Observed Molecular Weight
110, 200 kDa
Calculated molecular weight
154.8 kDa
Background of IGF1R
IGF1R (Insulin-like Growth Factor 1 (IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1 (IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, Dey et al. (1998) identified a regulatory subunit of phosphatidylinositol (PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1746 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human SH-SY5Y whole cell, human A549 whole cell, mouse brain tissue
IHC: human intestinal cancer tissue, rat colon tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IGF1R using anti-IGF1R antibody (PA1746).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF1R antigen affinity purified polyclonal antibody (PA1746) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IGF1R at approximately 110, 200 kDa. The expected band size for IGF1R is at 155 kDa.
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IHC analysis of IGF1R using anti-IGF1R antibody (PA1746).
IGF1R was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1R Antibody (PA1746) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of IGF1R using anti-IGF1R antibody (PA1746).
IGF1R was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1R Antibody (PA1746) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-IGF1 Receptor/IGF1R Antibody Picoband® (PA1746)
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Customer Q&As
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19 Customer Q&As for Anti-IGF1 Receptor/IGF1R Antibody Picoband®
Question
We want using your anti-IGF1 Receptor/IGF1R antibody for glucose homeostasis studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We appreciate your inquiry. This PA1746 anti-IGF1 Receptor/IGF1R antibody is tested on 293t cell lysate, a549 cell lysate, rat pancreas tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-13
Question
We want to test anti-IGF1 Receptor/IGF1R antibody PA1746 on rat fetal brain for research purposes, then I may be interested in using anti-IGF1 Receptor/IGF1R antibody PA1746 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The products we sell, including anti-IGF1 Receptor/IGF1R antibody PA1746, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-29
Question
Will anti-IGF1 Receptor/IGF1R antibody PA1746 work for WB with fetal brain?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
According to the expression profile of fetal brain, IGF1R is highly expressed in fetal brain. So, it is likely that anti-IGF1 Receptor/IGF1R antibody PA1746 will work for WB with fetal brain.
Boster Scientific Support
Answered: 2020-01-13
Question
Does anti-IGF1 Receptor/IGF1R antibody PA1746 work on zebrafish WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
Our lab technicians have not validated anti-IGF1 Receptor/IGF1R antibody PA1746 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IGF1 Receptor/IGF1R antibody PA1746 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-07
Question
Here is the WB image, lot number and protocol we used for fetal brain using anti-IGF1 Receptor/IGF1R antibody PA1746. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-28
Question
We are currently using anti-IGF1 Receptor/IGF1R antibody PA1746 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The anti-IGF1 Receptor/IGF1R antibody (PA1746) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-21
Question
Will PA1746 anti-IGF1 Receptor/IGF1R antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
You can see on the product datasheet, PA1746 anti-IGF1 Receptor/IGF1R antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-20
Question
I have a question about product PA1746, anti-IGF1 Receptor/IGF1R antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1746 anti-IGF1 Receptor/IGF1R antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-24
Question
Do you have a BSA free version of anti-IGF1 Receptor/IGF1R antibody PA1746 available?
T. Huang
Verified customer
Asked: 2019-04-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IGF1 Receptor/IGF1R antibody PA1746 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-09
Question
I was wanting to use your anti-IGF1 Receptor/IGF1R antibody for WB for rat fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fetal brain identification?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
It shows on the product datasheet, PA1746 anti-IGF1 Receptor/IGF1R antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-02
Question
Is a blocking peptide available for product anti-IGF1 Receptor/IGF1R antibody (PA1746)?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
We do provide the blocking peptide for product anti-IGF1 Receptor/IGF1R antibody (PA1746). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-10-30
Question
Will PA1746 cross-react with other human receptors like IRS Insulin Receptor Substrates?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
PA1746 does not react with IRS1, we do not have other relevant protein to verify the cross reactivity.
Boster Scientific Support
Answered: 2018-10-25
Question
We have been able to see staining in rat melanocyte. Are there any suggestions? Is anti-IGF1 Receptor/IGF1R antibody supposed to stain melanocyte positively?
H. Baker
Verified customer
Asked: 2018-08-17
Answer
Based on literature melanocyte does express IGF1R. Based on Uniprot.org, IGF1R is expressed in caput epididymis, placenta, fetal brain, melanocyte, cervix carcinoma, among other tissues. Regarding which tissues have IGF1R expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Fetal brain, Pubmed ID: 16572171
Melanocyte, Pubmed ID: 8247543, 1846292
Placenta, Pubmed ID: 2877871, 8257688
Boster Scientific Support
Answered: 2018-08-17
Question
My team were well pleased with the WB result of your anti-IGF1 Receptor/IGF1R antibody. However we have seen positive staining in melanocyte cell membrane using this antibody. Is that expected? Could you tell me where is IGF1R supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
Based on literature, melanocyte does express IGF1R. Generally IGF1R expresses in cell membrane. Regarding which tissues have IGF1R expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Fetal brain, Pubmed ID: 16572171
Melanocyte, Pubmed ID: 8247543, 1846292
Placenta, Pubmed ID: 2877871, 8257688
Boster Scientific Support
Answered: 2018-08-02
Question
We bought anti-IGF1 Receptor/IGF1R antibody for WB on caput epididymis a few months ago. I am using rat, and We want to use the antibody for IHC next. Our lab want to know about examining caput epididymis as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-05-11
Answer
I looked at the website and datasheets of our anti-IGF1 Receptor/IGF1R antibody and it seems that PA1746 has been tested on rat in both WB and IHC. Thus PA1746 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-05-11
Question
I see that the anti-IGF1 Receptor/IGF1R antibody PA1746 works with WB, what is the protocol used to produce the result images on the product page?
N. Patel
Verified customer
Asked: 2014-10-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-10-29
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-IGF1 Receptor/IGF1R antibody PA1746. Let me know if you need anything else.
S. Mitchell
Verified customer
Asked: 2014-10-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-08
Question
A client of ours is interested in your PA1746 IGF1R Antibody, he needs to know if this IGF1 Human Receptor cross-react with other human receptors like IRS Insulin Receptor Substrates. Did you ever make any specific tests on this concern? Can you indicate if there is publication regarding this cross-reaction?
Verified Customer
Verified customer
Asked: 2014-04-28
Answer
Test result shows that PA1746 does not react with IRS1. This is what we can do right now. We haven't got more relevant protein to verify the cross reactivity. PA2269 IRS1 ac3 PB9223 IRS1 BP71 PB9224 IRS2 BP69
Boster Scientific Support
Answered: 2014-04-28
Question
Is this PA1746 anti-IGF1 Receptor/IGF1R antibody reactive to the isotypes of IGF1R?
S. Bhatt
Verified customer
Asked: 2013-09-25
Answer
The immunogen of PA1746 anti-IGF1 Receptor/IGF1R antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor(1353-1367aa RKNERALPLPQSSTC), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-09-25
