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Product Info Summary
| SKU: | M02010 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-IGF2BP2 Mouse Monoclonal Antibody [Clone ID: OTI3F9]
SKU/Catalog Number
M02010
Size
100 µl
Description
Boster Bio Anti-IGF2BP2 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9). Catalog# M02010. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IGF2BP2 Mouse Monoclonal Antibody [Clone ID: OTI3F9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02010)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3F9
Isotype
IgG1
Immunogen
Full length human recombinant protein of human IGF2BP2 (NP_006333) produced in HEK293T cell.
Reactive Species
M02010 is reactive to IGF2BP2 in Dog, Human, Monkey, Mouse, Rat
Calculated molecular weight
66.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02010 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000~2000
IHC 1:50
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.
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Figure from citation: Western Blot of IGF2BP2 (IMP2) protein level by using anti-IGF2BP2 antibody in rat glomerular MCs.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either IGF2BP2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody (M02010)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-IGF2BP2 mouse monoclonal antibody (M02010) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.
Specific Publications For Anti-IGF2BP2 Mouse Monoclonal Antibody [Clone ID: OTI3F9] (M02010)
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