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Product Info Summary
| SKU: | PB9193 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FGFR4 Antibody Picoband®
SKU/Catalog Number
PB9193
PB0189 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGFR4 Antibody Picoband® catalog # PB9193. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGFR4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9193)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FGFR4 recombinant protein (Position: L22-H206). Human FGFR4 shares 86% and 84% amino acid (aa) sequences identity with mouse and rat FGFR4, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9193 is reactive to FGFR4 in Human
Observed Molecular Weight
100 kDa
Calculated molecular weight
88.0 kDa
Background of FGFR4
FGFR4 (Fibroblast growth factor receptor 4), also known as CD334, is a protein that in humans is encoded by the FGFR4 gene. It is mapped to 5q35.2. The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. It is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9193 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human PANC whole cell, human SGC whole cell, human COLO320 whole cell, human SW620 whole cell
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of FGFR4 using anti-FGFR4 antibody (PB9193).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human FGFR4 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 antigen affinity purified polyclonal antibody (Catalog # PB9193) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR4 at approximately 39 kDa. The expected band size for FGFR4 is at 39 kDa.
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Western blot analysis of FGFR4 using anti-FGFR4 antibody (PB9193).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PANC whole cell lysates,
Lane 3: human SGC whole cell lysates,
Lane 4: human COLO320 whole cell lysates,
Lane 5: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 antigen affinity purified polyclonal antibody (Catalog # PB9193) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR4 at approximately 100 kDa. The expected band size for FGFR4 is at 88 kDa.
Click image to see more details
IHC analysis of FGFR4 using anti-FGFR4 antibody (PB9193).
FGFR4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FGFR4 Antibody (PB9193) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-FGFR4 Antibody Picoband® (PB9193)
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1 Customer Q&As for Anti-FGFR4 Antibody Picoband®
Question
We are currently using anti-FGFR4 antibody PB9193 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The anti-FGFR4 antibody (PB9193) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
