Anti-IL1 beta/IL1B Antibody


SKU PA1533
Size 100μg/vial
Reactivity Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ELISA, WB

Overview

Product Name Anti-IL1 beta/IL1B Antibody
SKU/Catalog Number PA1533
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Interleukin-1 beta(IL1B) detection. Tested with WB, ELISA in Rat.
Cite This Product Anti-IL1 beta/IL1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1533)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of rat IL-1 beta(249-263aa FLGNSNGRDIVDFTM).
Reactivity Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ELISA , 0.5-1μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Anti- IL-1 beta antibody, PA1533, Western blotting
All lanes: Anti IL-1 beta (PA1533) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Predicted bind size: 31KD
Observed bind size: 31KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q63264
Gene Name Il1b
Protein Name Interleukin-1 beta
Alternative Names Interleukin-1 beta;IL-1 beta;Il1b;
Subcellular Localization Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Molecular Weight 30644 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Research Areas Rat

*You can search these to find other products in these research areas.
Background Interleukin-1 beta(IL-1beta) also know as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene, which localizes to the long arm of chromosome 2 at position 2q13-2q21 between two fragile sites. Interleukin 1(IL-1) is a protein with several biological activities regulating host defense and immune responses. The human IL-1 family plays an important role in the pathogenesis of many diseases and functions as a key mediator of the host response to various infectious, inflammatory, and immunologic challenges.

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Polyclonal antibody for IL 1 BETA/IL1B detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Rat. IL 1 BETA/IL1B information: Molecular Weight: 30644 MW; Subcellular Localization: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
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In stock
Order Product
PA1533
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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Publications

Efficacy of acetylshikonin in preventing obesity and hepatic steatosis in db/db mice
Exogenous %u03B1-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats
In vivo anti-inflammatory activity of caffeoylquinic acid derivatives from Solidago virgaurea in rats
In vivo anti-inflammatory activity of caffeoylquinic acid derivatives from Solidago virgaurea in rats
Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?
NLRP3 Inflammasome Sequential Changes in?Staphylococcus aureus-Induced Mouse Model of Acute Rhinosinusitis
Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke
Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to il 1b antibody, il 1 antibody, il 1 beta antibody, il1 beta antibody, il-1 beta antibody, il1b antibody, interleukin 1 beta antibody, interleukin-1 beta antibody
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