Anti-IL-1 beta/IL1B Antibody Picoband™

IL-1 beta/IL-1F2 antibody

Boster Bio Anti-IL-1 beta/IL1B Antibody Picoband™ catalog # A00101. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 27 publication(s).

Product Info Summary

SKU: A00101
Size: 100ug/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-IL-1 beta/IL1B Antibody Picoband™

View all IL-1 beta/IL-1F2 Antibodies

SKU/Catalog Number

A00101

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-IL-1 beta/IL1B Antibody Picoband™ catalog # A00101. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL-1 beta/IL1B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00101)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human IL-1 beta recombinant protein (Position: A117-S269). Human IL-1 beta shares 78.3% and 77.6% amino acid (aa) sequence identity with mouse and rat IL-1 beta, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00101 is reactive to IL1B in Human, Mouse, Rat

Applications

A00101 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For IL1B (Source: Uniprot.Org, NCBI)

Uniprot ID

P01584

Gene ID

3553

Gene Name

IL1B

Full Name

Interleukin-1 beta

Weight

30.748kDa

Superfamily

IL-1 family

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1B, check out the IL1B Infographic

IL1B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00101 has been cited in 27 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα

The interrupted cross-talk of inflammatory and oxidative stress trajectories signifies the effect of artesunate against hepatic ischemia/reperfusion-induced inflammasomopathy

Neuregulin-1 protects mouse cerebellum against oxidative stress and neuroinflammation

Protective effects of tannic acid on acute doxorubicin-induced cardiotoxicity: Involvement of suppression in oxidative stress, inflammation, and apoptosis

IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation

Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats

Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats

Shikonin protects against lipopolysaccharide-induced inflammation and apoptosis in human nucleus pulposus cells through the nuclear factor-kappa B pathway

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats

Protective effect of melatonin on cigarette smoke‐induced restenosis in rat carotid arteries after balloon injury
Species: Rat

Microstructural changes and immunohistological analysis of pro-inflammatory cytokines in spleens of lipopolysaccharide-induced rats.Indian Journal Of Animal Research.2019.(53):239-244
Species: Rat
A00101 usage in article: APP:IHC, SAMPLE:SPLEEN TISSUE, DILUTION:NA

Ghoneim ME,Abdallah DM,Shebl AM,El-Abhar HS. The interrupted cross-talk of inflammatory and oxidative stress trajectories signifies the effect of artesunate against hepatic ischemia/reperfusion-induced inflammasomopathy. Toxicol Appl Pharmacol.2020 Oct 29
Species: Rat
A00101 usage in article: APP:WB, SAMPLE:PROTEIN EXTRACTIONS OF LIVER TISSUES, DILUTION:1:300

Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis infection in human macrophage cells

Exogenous %u03B1-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats

In vivo anti-inflammatory activity of caffeoylquinic acid derivatives from Solidago virgaurea in rats

Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?

Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation

NLRP3 Inflammasome Sequential Changes in?Staphylococcus aureus-Induced Mouse Model of Acute Rhinosinusitis

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.

Tan Wf, Cao Xz, Wang Jk, Lv Hw, Wu By, Ma H. Clin Exp Pharmacol Physiol. 2010 Oct;37(10):1010-5. Doi: 10.1111/J.1440-1681.2010.05433.X. Protective Effects Of Lithium Treatment For Spatial Memory Deficits Induced By Tau Hyperphosphorylation In Sple...

Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Int J Mol Sci. 2012;13(7):8379-87. Doi: 10.3390/Ijms13078379. Epub 2012 Jul 5. Astragalus Membranaceus Inhibits Inflammation Via Phospho-P38 Mitogen-Activated Protein Kinase (Mapk) And Nuclear Factor (Nf)-...

Li J, Zhao L, He X, Zeng Yj, Dai Ss. Plos One. 2013;8(3):E59257. Doi: 10.1371/Journal.Pone.0059257. Epub 2013 Mar 15. Sinomenine Protects Against Lipopolysaccharide-Induced Acute Lung Injury In Mice Via Adenosine A(2A) Receptor Signaling.

Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...

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17 Customer Q&As for Anti-IL-1 beta/IL1B Antibody Picoband™

Question

Will A00101 anti-IL-1 beta/IL1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

You can see on the product datasheet, A00101 anti-IL-1 beta/IL1B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-28

Question

Is this A00101 anti-IL-1 beta/IL1B antibody reactive to the isotypes of IL1B?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

The immunogen of A00101 anti-IL-1 beta/IL1B antibody is E. coli-derived human IL-1 beta recombinant protein (Position: A117-S269). Human IL-1 beta shares 78.3% and 77.6% amino acid (aa) sequence identity with mouse and rat IL-1 beta, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-14

Question

We are currently using anti-IL-1 beta/IL1B antibody A00101 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

The anti-IL-1 beta/IL1B antibody (A00101) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-30

Question

I would like to test anti-IL-1 beta/IL1B antibody A00101 on rat leukocyte for research purposes, then I may be interested in using anti-IL-1 beta/IL1B antibody A00101 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

The products we sell, including anti-IL-1 beta/IL1B antibody A00101, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-04

Question

We have seen staining in rat skin. Do you have any suggestions? Is anti-IL-1 beta/IL1B antibody supposed to stain skin positively?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

According to literature skin does express IL1B. According to Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436

Boster Scientific Support

Answered: 2019-08-23

Question

Is a blocking peptide available for product anti-IL-1 beta/IL1B antibody (A00101)?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

We do provide the blocking peptide for product anti-IL-1 beta/IL1B antibody (A00101). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-20

Question

Do you have a BSA free version of anti-IL-1 beta/IL1B antibody A00101 available?

G. Krishna

Verified customer

Asked: 2019-06-19

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-1 beta/IL1B antibody A00101 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-19

Question

I see that the anti-IL-1 beta/IL1B antibody A00101 works with WB, what is the protocol used to produce the result images on the product page?

C. Patel

Verified customer

Asked: 2019-05-29

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-29

Question

Do you know if A00101 recognizes the native or denatured target?

Verified customer

Asked: 2019-05-06

Answer

Yes, the Anti-IL-1 Beta/IL1B Antibody Picoband™ (A00101) recognizes the denatured target.

Boster Scientific Support

Answered: 2019-05-07

Question

Please see the WB image, lot number and protocol we used for leukocyte using anti-IL-1 beta/IL1B antibody A00101. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-04-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-16

Question

Checking back with a new antibody questions - wondering if the one I recently found below has been characterized to determine if it recognizes native or denatured target? https://www.bosterbio.com/anti-il-1-beta-picoband-trade-antibody-a00101-boster.html?options=cart#gene-info Thanks! Janice

Verified Customer

Verified customer

Asked: 2019-03-29

Answer

This antibody recognizes the denatured target

Boster Scientific Support

Answered: 2019-03-29

Question

I was wanting to use your anti-IL-1 beta/IL1B antibody for WB for rat leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukocyte identification?

Verified Customer

Verified customer

Asked: 2018-08-09

Answer

As indicated on the product datasheet, A00101 anti-IL-1 beta/IL1B antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-09

Question

Would anti-IL-1 beta/IL1B antibody A00101 work for WB with leukocyte?

Verified Customer

Verified customer

Asked: 2017-11-21

Answer

According to the expression profile of leukocyte, IL1B is highly expressed in leukocyte. So, it is likely that anti-IL-1 beta/IL1B antibody A00101 will work for WB with leukocyte.

Boster Scientific Support

Answered: 2017-11-21

Question

My question regarding product A00101, anti-IL-1 beta/IL1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-26

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00101 anti-IL-1 beta/IL1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-26

Question

I was wanting to use using your anti-IL-1 beta/IL1B antibody for response to lipopolysaccharide studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-07-04

Answer

Thank you for your inquiry. This A00101 anti-IL-1 beta/IL1B antibody is validated on rat spleen tissue, tissue lysate, mouse nih3t3 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-07-04

Question

Our lab were content with the WB result of your anti-IL-1 beta/IL1B antibody. However we have observed positive staining in macrophage cytoplasm using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-05-31

Answer

According to literature, macrophage does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436

Boster Scientific Support

Answered: 2017-05-31

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-IL-1 beta/IL1B antibody A00101. Let me know if you need anything else.

K. Yang

Verified customer

Asked: 2016-10-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-10-20

Conjugation

how to order through PO

Size

Total: $315

Size:100ug

SKU:A00101

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A00101
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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