Anti-IL-1 beta/IL1B Antibody Picoband®

Inhibition of GSDMD activation suppressed the expression of pyroptosis pathway-related proteins in ApoE −/− mice. After 4 weeks of treatment the ApoE −/− mice were killed at 18 weeks. (A) Western blotting analysis was conducted to detect protein levels of NLRP3, caspase-1, GSDMD, cleaved IL-1β, and HMGB1 in the aorta. (B–F) Quantitative analysis of expression of NLRP3, p10 (caspase-1), GSDMD-N, cleaved IL-1β, and HMGB1 ( n = 6). β-actin served as the loading control. Data represent means ± SEM.
Index in PubMed under a CC BY license. PMID: 37593179

Z-LLSD-FMK or Z-YVAD-FMK inhibited GSDMD activation or pyroptosis induced by LPS + nigericin in BMDMs. BMDMs were primed with LPS for 4 h followed by nigericin for 30 min. Z-LLSD-FMK, Z-YVAD-FMK, and both combined were added 30 min before LPS + nigericin treatment. (A) Representative immunofluorescence images of cell death determination via PI (red) and Hoechst 33342 (blue) staining (scale bar = 75 μm). (B) The percentage of PI-positive BMDMs was calculated at five randomly selected image fields ( n = 5). (C) LDH release in supernatants ( n = 5). (D) IL-1β release in supernatants was analyzed by ELISA ( n = 5). (E) Western blotting analysis was performed to detect protein levels of NLRP3, caspase-1, GSDMD, cleaved IL-1β, and HMGB1 in supernatants and cell lysates. (F–J) Quantitative analysis of expression of NLRP3, p10 (caspase-1), GSDMD-N, cleaved IL-1β, and HMGB1 in cell lysates ( n = 5). β-actin served as the loading control. Data represent means ± SEM.
Index in PubMed under a CC BY license. PMID: 37593179

The effects of P2Y 14 shRNA and naringin on the expression of IL-1β in the SCG of type 2 diabetic rats. The expression level of IL-1β protein was determined by Western blotting (A) . The bar histogram displays the IOD ratio of IL-1β protein mass to β-actin protein mass in each group (B) , and the values are the mean ± SEM from three independent experiments. *** p < 0.001 vs. Ctrl; # p < 0.05 vs. DM; ## p < 0.01 vs. DM.
Index in PubMed under a CC BY license. PMID: 35529431

Effects of polysaccharide extract from XJEK on TNF-α, IL-1β and IL-10 of HUVECs induced by Ang II. ( a ) TNF-α level in supernatants of HUVECs; ( b ) IL-1β level in supernatants of HUVECs; ( c ) IL-10 level in supernatants of HUVECs. 1, blank control group; 2, Ang II (10 − 5 mol/L) group; 3, Ang II (10 − 5 mol/L) + AqE (0.15 mg/ml) group; 4, Ang II (10 − 5 mol/L) + AqE (0.3 mg/ml) group; 5, Ang II (10 − 5 mol/L) + AqE (0.6 mg/ml) group; 6, Ang II (10 − 5 mol/L) + AqE (1.2 mg/ml) group; 7, Ang II (10 − 5 mol/L) + XJEK (1.6 mg/ml) group. Data are expressed as mean ± SD, n = 6. ** P < 0.01 vs control group; ## P < 0.01 vs Ang II group
Index in PubMed under a CC BY license. PMID: 31196042

Effects of polysaccharide extract from XJEK on TNF-α, IL-1β and IL-10 in L -NAME-induced hypertensive mice. ( a ) TNF-α expression level in plasma. ( b ) IL-1β expression level in plasma. ( c ) IL-10 expression level in plasma. ( d ) IL-1β expression level in cardiac tissues. ( e ) TNF-α expression level in cardiac tissues. ( f ) IL-10 expression level in cardiac tissues. ( g ) Representative image of IL-1β immunocytochemistry. ( h ) Representative image of TNF-α immunocytochemistry.( i ) Representative image of IL-10 immunocytochemistry.1,negative group; 2,control group; 3, model group; 4, L -NAME+AqE group; 5, L -NAME+XJEK group; 6, L -NAME+fosinopril group. Data are presented as the mean ± SD ( n = 10). ** P < 0.01 vs. control group; # P < 0.05, ## P < 0.01 vs, model group
Index in PubMed under a CC BY license. PMID: 31196042

Western blot analysis of IL1B using anti-IL1B antibody (A00101).
Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant rat IL1B protein 10 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL1B at approximately 17 kDa.

Western blot analysis of IL1B using anti-IL1B antibody (A00101).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7(-LPS) whole cell lysates,
Lane 2: mouse RAW264.7(+LPS) whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL1B at approximately 31-35 kDa. The expected band size for IL1B is at 31 kDa.

Western blot analysis of IL1B using anti-IL1B antibody (A00101).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: control group-normal mouse hippocampal tissue lysates,
Lane 2: hippocampal tissue from Alzheimer’s disease model mouse,
Lane 3: hippocampal tissue from Alzheimer’s disease model mouse treated with a self-developed drug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified polyclonal antibody (Catalog # A00101) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for IL1B at approximately 31-35 kDa. The expected band size for IL1B is at 31 kDa.