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IL1B antibody (A00101) performs well in WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 53 Publications

a00101 il1b primary antibodies wb testing 1

Western blot analysis of IL1B using anti-IL1B antibody (A00101).
Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant rat IL1B protein 10 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL1B at approximately 17 kDa.

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Anti-IL-1 beta/IL1B Antibody Picoband®

Name: Anti-IL-1 beta/IL1B Antibody Picoband®
Brand: Boster Bio
SKU: A00101
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

IL1B antibody

53 Publications|

2 Reviews

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	WB

Tested Applications

IL1B antibody was positively detected by WB in:

recombinant rat IL1B protein, mouse RAW264.7 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

Mouse

Tested

Human

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-IL-1 beta/IL1B Antibody Picoband®
View all IL1B Antibodies

Form

Lyophilized

Uniprot

P01584

Gene ID

3553

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human IL-1 beta recombinant protein (Position: A117-S269). Human IL-1 beta shares 78.3% and 77.6% amino acid (aa) sequence identity with mouse and rat IL-1 beta, respectively.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

31-35 kDa

Calculated Molecular Weight

30.7 kDa

Background of IL1B

FGFR1, Fibroblast growth factor receptor 1, also known as basic fibroblast growth factor receptor 1, fms-related tyrosine kinase-2 / Pfeiffer syndrome, and CD331, is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. The FGFR1 gene is localized to 8p12-p11.2 by in situ hybridization. FGFR1 is essential for the normal formation of the organ of Corti and that phenotype severity observed in FGFR1 mutants is dependent on the dose of FGFR1. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome 2.

Cite This Product

Anti-IL-1 beta/IL1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00101)

Protocols

Western Blot protocol for IL1B antibody

Review Protocol

Publications

Perfluorohexane/hemoglobin nano-oxygen carriers enhance transplanted islet graft survival via hypoxia alleviation and mitochondrial repair

Published: 2026-01-19

Journal: JOURNAL OF CONTROLLED RELEASE

Application: IF

Impact Factor: 11.50

Species: Mouse

PMID: 41565185

SKU	A00101
Application	Western Blot
Sample	PC-12 (rat adrenal pheochromocytoma cells, highly differentiated)
Sample Processing Description	① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	IL-1 beta/IL1B Antibody Picoband®
Primary Incubation	1:2500, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. IL1β is a typical pro-inflammatory factor. The experimental results showed that the target band was clear, the total IL1β protein expression was upregulated after modeling, and the expression level decreased after drug treatment, which was in line with expectations.

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Anti-IL-1 beta/IL1B Antibody Picoband®

IL1B antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Perfluorohexane/hemoglobin nano-oxygen carriers enhance transplanted islet graft survival via hypoxia alleviation and mitochondrial repair

Low-intensity pulsed ultrasound ameliorates partial infraorbital nerve ligation-induced trigeminal neuropathic pain through inhibiting Schwann cell Pannexin 1 channel

Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells

Hyperbaric oxygen ameliorates neuroinflammation in heat-stressed BV-2 microglial cells: potential involvement of EAAT2 regulation

Ustiloxins induce kidney injury via the TLR2/MAPK/NF-κB pathway

Novel microRNA seq-14465_x69 promotes scarless wound healing by targeting TGF-β/SMADs pathway in keratinocytes

miR-708-5p Attenuates Osteoarthritis Progression via Multi-Target Modulation of the NOX4/NF-κB Axis and Cartilage Homeostasis

A self-assembled nano-spray formulation for synergistic therapy of anti-inflammation and skin repair

Investigating the molecular mechanism of epimedium herb in treating rheumatoid arthritis through network pharmacology, molecular docking, and experimental validation

DJ-1 regulates astrocyte activation through miR-155/SHP-1 signaling in cerebral ischemia/reperfusion injury

Disturbance of mitochondrial dynamics led to spermatogenesis disorder in mice exposed to polystyrene micro- and nanoplastics

Hyperbaric oxygen therapy attenuates heatstroke-induced hippocampal injury by inhibiting microglial pyroptosis

LMWH prevents thrombo-inflammation in the placenta via HBEGF-AKT signalling

Using broadly targeted plant metabolomics technology combined with network pharmacology to explore the mechanism of action of the Yishen Gushu formula in the treatment of postmenopausal osteoporosis in vivo

Penicilazaphilone C alleviates allergic airway inflammation and improves the immune microenvironment by hindering the NLRP3 inflammasome

Influences of Heat Stress on Glutamate Transmission-Dependent Expression Levels of IL-1β and IL-18 in BV-2 Microglial Cells

Carnosine ameliorates postoperative cognitive dysfunction of aged rats by limiting astrocytes pyroptosis

Fructus lycii oligosaccharide alleviates acute liver injury via PI3K/Akt/mTOR pathway

Rutaecarpine Ameliorates Murine N-Methyl-N’-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis by Sonic Hedgehog Pathway

Cocktail of three isoquinoline alkaloids derived from Glaucium grandiflorum Boiss. & A. Huet subsp. refractum (Nábelek) Mory inhibits the production of LPS-induced ROS, pro-inflammatory cytokines, and mediators through the down-regulation of p38 MAPK in B

Inhibition of GSDMD activation by Z-LLSD-FMK or Z-YVAD-FMK reduces vascular inflammation and atherosclerotic lesion development in ApoE−/− mice

Suramin ameliorates osteoarthritis by acting on the Nrf2/HO-1 and NF-κB signaling pathways in chondrocytes and promoting M2 polarization in macrophages

The inhibition of TRPML1/TFEB leads to lysosomal biogenesis disorder, contributes to developmental fluoride neurotoxicity

Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways

Human Neural Stem Cell Secretome Inhibits Lipopolysaccharide-Induced Neuroinflammation Through Modulating Microglia Polarization by Activating Peroxisome Proliferator-Activated Receptor Gamma

Naringin Relieves Diabetic Cardiac Autonomic Neuropathy Mediated by P2Y14 Receptor in Superior Cervical Ganglion

Manganese Carbonyl-Loaded Hollow Mesoporous Silica Nanoparticles Coated with Neutrophil Membranes for Acute Kidney Injury Therapy

Long non-coding RNA MSTRG.81401 short hairpin RNA relieves diabetic neuropathic pain and behaviors of depression by inhibiting P2X4 receptor expression in type 2 diabetic rats

Shikonin protects against lipopolysaccharide-induced inflammation and apoptosis in human nucleus pulposus cells through the nuclear factor-kappa B pathway

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats

The interrupted cross-talk of inflammatory and oxidative stress trajectories signifies the effect of artesunate against hepatic ischemia/reperfusion-induced inflammasomopathy

TRPA1 regulates macrophages phenotype plasticity and atherosclerosis progression

The protective effects of polysaccharide extract from Xin-Ji-Er-Kang formula on Ang II-induced HUVECs injury, L-NAME-induced hypertension and cardiovascular remodeling in mice

Abnormal sympathetic activity after myocardial ischemia involving P2X4 in dorsal root ganglia

Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway

Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway

Ursolic acid improves diabetic nephropathy via suppression of oxidative stress and inflammation in streptozotocin-induced rats

Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats

Protective effects of tannic acid on acute doxorubicin-induced cardiotoxicity: Involvement of suppression in oxidative stress, inflammation, and apoptosis

Protective effects of tannic acid on pressure overload-induced cardiac hypertrophy and underlying mechanisms in rats

Neuregulin-1 protects mouse cerebellum against oxidative stress and neuroinflammation

RETRACTED: miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα

Cerebralcare Granule®, a Chinese Herb Compound Preparation, Attenuates d-Galactose Induced Memory Impairment in Mice

Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats

Green tea epigallocatechin-3-gallate alleviates Porphyromonas gingivalis-induced periodontitis in mice

IL-10 and IL-1β Mediate Neuropathic-Pain Like Behavior in the Ventrolateral Orbital Cortex

The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation

Ulinastatin attenuates renal interstitial inflammation and inhibits fibrosis progression in rats under unilateral ureteral obstruction

Melanocortin 4 receptor antagonists attenuates morphine antinociceptive tolerance, astroglial activation and cytokines expression in the spinal cord of rat

Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats

Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats

Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye

IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation

Review

Have you used Anti-IL-1 beta/IL1B Antibody Picoband®?

2 Reviews For Anti-IL-1 beta/IL1B Antibody Picoband®

IL-1β Antibody (A00101) showed a clear and specific band in PC-12 cells, with IL-1β expression increased after Aβ-induced AD modeling and reduced following drug treatment, consistent with the expected results.

IL-1β Antibody (A00101) shows clear, specific bands with low background in mouse brain tissues by WB, with elevated expression in Alzheimer’s disease models and reduced levels after treatment, demonstrating excellent antibody performance.

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