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Product Info Summary
| SKU: | PB9244 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL15 Antibody Picoband®
SKU/Catalog Number
PB9244
PB0249 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL15 Antibody Picoband® catalog # PB9244. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9244)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IL-15 recombinant protein (Position: N49-S162). Human IL-15 shares 70% amino acid (aa) sequence identity with both mouse and rat IL-15.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9244 is reactive to IL15 in Human
Observed Molecular Weight
15 kDa
Calculated molecular weight
18.1 kDa
Background of IL15
Interleukin (IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. This gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might be beneficial in the prevention of acute, and especially chronic, allograft rejection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9244 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-IL-15 antibody, PB9244, Western blotting
All lanes: Anti IL-15 (PB9244) at 0.5ug/ml
WB: Recombinant Human IL-15 Protein 0.5ng
Predicted bind size: 15KD
Observed bind size: 15KD
Click image to see more details
Construction and expression of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. a Schematic diagram of the two IL-15/SuIL-15Rα-IgG4 Fc complexes. IL-15/SuIL-15Rα-dFc was composed of two molecules of mutated IL-15 noncovalently bound to a modified dimeric SuIL-15Rα-IgG4 Fc fusion protein, while IL-15/SuIL-15Rα-mFc consisted of a single molecule of mutated IL-15 noncovalently bound to a modified monomeric SuIL-15Rα-IgG4 Fc fusion protein. b The construction strategy of the two IL-15/SuIL-15Rα-IgG4 Fc complexes. pPIC9-SuIL-15Rα-dFc or pPIC9-SuIL-15Rα-mFc and pPICZα-IL-15 were inserted into the genome of GS115 through homologous recombination using the his4 and AOX1 sequences, respectively. c Screening for the expression of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. Expression clones were first screened by dot blotting using rabbit anti-human IL-15 antibody followed by donkey anti-rabbit IgG-HRP conjugate, and then high expression clones were further selected and confirmed by Western blotting under nonreducing conditions using anti-human IgG4-HRP conjugate. Clones No. 15–2 and 62–4 were selected as the expression clones used in pilot-scale fermentation of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc, respectively. The numbers in the figure represent the clone numbers
Index in PubMed under a CC BY license. PMID: 34107983
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Pilot-scale fermentation, purification and characterization of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. a Representative three-step fermentation process, including batch phase, glycerol fed-batch phase and methanol induction phase. Parameters, such as dissolved oxygen (DO), feeding speed, temperature and pH, were monitored during fermentation. b Cell growth of the two IL-15/SuIL-15Rα-IgG4 Fc complex-expressing strains during fermentation was monitored and represented as wet cell weight. c The expression of the two IL-15/SuIL-15Rα-IgG4 Fc complexes during fermentation was analyzed by Western blotting using an anti-human IgG4-HRP conjugate under nonreducing conditions. d The downstream processing workflow of fermentation broth. e The two purified IL-15/SuIL-15Rα-IgG4 Fc complexes were separated by SDS-PAGE under reducing conditions and identified by Western blotting or Coomassie blue staining. The contents within the dashed boxes are the presumed protein structure of the corresponding bands. M: prestained protein marker; dFc: IL-15/SuIL-15Rα-dFc; mFc: IL-15/SuIL-15Rα-mFc
Index in PubMed under a CC BY license. PMID: 34107983
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In vitro bioactivity of rhIL-15, IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. A CTLL-2 cell proliferation assay was used to evaluate the biological activity of the rhIL-15 and IL-15/SuIL-15Rα-IgG4 Fc complexes. The EC 50 values were calculated using the four-parameter nonlinear logistic regression model. All data points are the means ± standard deviation of triplicate OD values. The results are representative of at least three experiments
Index in PubMed under a CC BY license. PMID: 34107983
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Pharmacokinetics analysis of rhIL-15, IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. C57BL/6 J mice (male, 6–8 weeks of age, n = 4–9 for each group) were intravenously administered 0.28 mg/kg rhIL-15 or 1 mg/kg IL-15/SuIL-15Rα-IgG4 Fc complexes, and blood samples were collected at the indicated time points after injection. The concentration of IL-15 in serum was measured by ELISA. All data points are the means ± standard deviation of the concentrations
Index in PubMed under a CC BY license. PMID: 34107983
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In vivo bioactivity of rhIL-15, IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. C57BL/6 J mice (male, 6–8 weeks of age, n = 10–14 for each group) were intravenously injected with PBS, 0.28 mg/kg rhIL-15 or 1 mg/kg IL-15/SuIL-15Rα-IgG4 Fc complexes. Seventy-two hours after treatment, spleens and blood samples were collected and prepared for flow cytometry. a Representative photo of spleens separated from each group (n = 5). b Spleen weight statistics. c The percentage of each indicated cell subset in splenocytes. d The percentage of each indicated cell subset in peripheral blood cells. The data shown in b, c, and d are combined from three independent experiments, and all data points are the means ± standard deviation. ns, not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001 and ****: p < 0.0001
Index in PubMed under a CC BY license. PMID: 34107983
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Effects of rhIL-2, rhIL-15, IL-15/SuIL-15Rα-dFc, and IL-15/SuIL-15Rα-mFc on NK and CD8 + T cell proliferation. Human PBMCs from healthy donors (n = 6) were cultured for 7 days in the absence or presence of 50 IU/mL rhIL-2, 10 ng/mL rhIL-15 or 35.7 ng/mL IL-15/SuIL-15Rα-IgG4 Fc complexes, and the proliferation of CFSE + NK cells and CFSE + CD8 + T cells was measured by flow cytometry. a Results from one representative healthy donor. b Statistics on the proliferation of NK cells or CD8 + T cells from all healthy donors. All data points are means ± standard deviation. *: p < 0.05; **: p < 0.01; ***: p < 0.001
Index in PubMed under a CC BY license. PMID: 34107983
Specific Publications For Anti-IL15 Antibody Picoband® (PB9244)
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Customer Q&As
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15 Customer Q&As for Anti-IL15 Antibody Picoband®
Question
Is a blocking peptide available for product anti-IL15 antibody (PB9244)?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We do provide the blocking peptide for product anti-IL15 antibody (PB9244). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-16
Question
I am interested in to test anti-IL15 antibody PB9244 on human epidermis for research purposes, then I may be interested in using anti-IL15 antibody PB9244 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The products we sell, including anti-IL15 antibody PB9244, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-30
Question
Our lab used your anti-IL15 antibody for ELISA on decidua last year. I am using human, and We intend to use the antibody for WB next. you antibody examining decidua as well as lung cancer in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
I viewed the website and datasheets of our anti-IL15 antibody and I see that PB9244 has been validated on human in both ELISA and WB. Thus PB9244 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-02
Question
We are currently using anti-IL15 antibody PB9244 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The anti-IL15 antibody (PB9244) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
Is this PB9244 anti-IL15 antibody reactive to the isotypes of IL15?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
The immunogen of PB9244 anti-IL15 antibody is E.coli-derived human IL-15 recombinant protein (Position: N49-S162). Human IL-15 shares 70% amino acid (aa) sequence identity with both mouse and rat IL-15. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epidermis using anti-IL15 antibody PB9244. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-06
Question
Can you help my question with product PB9244, anti-IL15 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9244 anti-IL15 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-04
Question
Will anti-IL15 antibody PB9244 work for ELISA with epidermis?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
According to the expression profile of epidermis, IL15 is highly expressed in epidermis. So, it is likely that anti-IL15 antibody PB9244 will work for ELISA with epidermis.
Boster Scientific Support
Answered: 2019-11-01
Question
We were well pleased with the WB result of your anti-IL15 antibody. However we have observed positive staining in cerebellum heart isoform il15-s21aa: cytoplasm. nucleus. using this antibody. Is that expected? Could you tell me where is IL15 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
From literature, cerebellum heart does express IL15. Generally IL15 expresses in isoform il15-s48aa: secreted., isoform il15-s21aa: cytoplasm. nucleus. Regarding which tissues have IL15 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 8178155
Cerebellum, and Heart, Pubmed ID: 14702039
Colon, Pubmed ID: 15489334
Epidermis, Pubmed ID: 15123770
Lung cancer, Pubmed ID: 8668345
Testis, Pubmed ID: 9405632
Boster Scientific Support
Answered: 2019-08-30
Question
Here is the WB image, lot number and protocol we used for epidermis using anti-IL15 antibody PB9244. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-15
Question
We have observed staining in human colon. Any tips? Is anti-IL15 antibody supposed to stain colon positively?
L. Yang
Verified customer
Asked: 2018-04-13
Answer
Based on literature colon does express IL15. Based on Uniprot.org, IL15 is expressed in decidua, bone marrow, lung cancer, testis, cerebellum heart, colon, epidermis, among other tissues. Regarding which tissues have IL15 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 8178155
Cerebellum, and Heart, Pubmed ID: 14702039
Colon, Pubmed ID: 15489334
Epidermis, Pubmed ID: 15123770
Lung cancer, Pubmed ID: 8668345
Testis, Pubmed ID: 9405632
Boster Scientific Support
Answered: 2018-04-13
Question
I see that the anti-IL15 antibody PB9244 works with ELISA, what is the protocol used to produce the result images on the product page?
A. Krishna
Verified customer
Asked: 2018-03-21
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-21
Question
I was wanting to use your anti-IL15 antibody for ELISA for human epidermis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human epidermis identification?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
As indicated on the product datasheet, PB9244 anti-IL15 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human epidermis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-08
Question
Will PB9244 anti-IL15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-18
Answer
It shows on the product datasheet, PB9244 anti-IL15 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-18
Question
Is there a BSA free version of anti-IL15 antibody PB9244 available?
S. Wu
Verified customer
Asked: 2014-01-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL15 antibody PB9244 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-01-30
