Click image to see more details
Product Info Summary
| SKU: | A00102-3 |
|---|---|
| Size: | 1mL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IL-6 Antibody
SKU/Catalog Number
A00102-3
Size
1mL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-IL-6 Antibody (Catalog # A00102-3). Tested in Dot blot, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL-6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00102-3)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
A00102-3 is reactive to IL6 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
23.7 kDa
Background of IL6
Anti-IL-6 Antibody recognizes human IL-6 that is a secreted cytokine with a wide variety of biological functions. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL6 antibody is ideal for investigators involved in Immunology and Cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00102-3 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:5,000
IHC: 1:400 - 1:800
IP: 1:400 - 1:800
WB: 1:500 - 1:2,000
Neutralization: 1:400
IL-6 antibody has been tested in western blot. This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, and immunoprecipitation. Reactivity in other immunoassays is unknown. Expect ~23.7kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. For immunoblot use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10ng/mL E. coli LPS. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. The antiserum is useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.
Validation Images & Assay Conditions
Click image to see more details
Western blot using Boster's Anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated Goat anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
Click image to see more details
Western Blot of Rabbit Anti-IL-6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker . Lane 2: Human Rec. IL-6/HeLa Whole Cell Lysate [0.05µg/10µg]. Lane 3: Human Rec. IL-6/HeLa Whole Cell Lysate [0.02µg/10µg]. Lane 4: Human Rec. IL-6/HeLa Whole Cell Lysate [0.01µg/10µg]. Lane 5: HeLa Whole Cell Lysate [10µg]. Primary Antibody: Anti-IL-6 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugate at 1:70,000 for 30mins at RT. Block: BlockOut Buffer . Predicted MW: ~23kDa. Observed MW: ~19 Exposure: 2 seconds.
Specific Publications For Anti-IL-6 Antibody (A00102-3)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IL-6 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-IL-6 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-IL-6 Antibody
Question
I was wanting to use your anti-IL-6 antibody for WB for human fibroblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fibroblast identification?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
You can see on the product datasheet, A00102-3 anti-IL-6 antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fibroblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-27
Question
Is this A00102-3 anti-IL-6 antibody reactive to the isotypes of IL6?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
The immunogen of A00102-3 anti-IL-6 antibody is This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-09
Question
We purchased anti-IL-6 antibody for WB on fibroblast in the past. I am using human, and We want to use the antibody for IP next. My lab would like examining fibroblast as well as left coronary artery in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
I looked at the website and datasheets of our anti-IL-6 antibody and I see that A00102-3 has been tested on human in both WB and IP. Thus A00102-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-17
Question
I see that the anti-IL-6 antibody A00102-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-09
Question
Is a blocking peptide available for product anti-IL-6 antibody (A00102-3)?
C. Johnson
Verified customer
Asked: 2019-10-02
Answer
We do provide the blocking peptide for product anti-IL-6 antibody (A00102-3). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-02
Question
Does anti-IL-6 antibody A00102-3 work for WB with fibroblast?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
According to the expression profile of fibroblast, IL6 is highly expressed in fibroblast. So, it is likely that anti-IL-6 antibody A00102-3 will work for WB with fibroblast.
Boster Scientific Support
Answered: 2019-08-19
Question
Can you help my question with product A00102-3, anti-IL-6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00102-3 anti-IL-6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-02
Question
Is there a BSA free version of anti-IL-6 antibody A00102-3 available?
J. Patel
Verified customer
Asked: 2018-12-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-6 antibody A00102-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-14
Question
Will A00102-3 anti-IL-6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Kulkarni
Verified customer
Asked: 2018-02-15
Answer
You can see on the product datasheet, A00102-3 anti-IL-6 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-15
Question
We are currently using anti-IL-6 antibody A00102-3 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-06
Answer
The anti-IL-6 antibody (A00102-3) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-06
Question
I have attached the WB image, lot number and protocol we used for fibroblast using anti-IL-6 antibody A00102-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-28
Question
Our lab were well pleased with the WB result of your anti-IL-6 antibody. However we have observed positive staining in fibroblast secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
According to literature, fibroblast does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-11-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-IL-6 antibody A00102-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-22
Question
We have seen staining in human left coronary artery. Any tips? Is anti-IL-6 antibody supposed to stain left coronary artery positively?
T. Rodriguez
Verified customer
Asked: 2016-01-05
Answer
From literature left coronary artery does express IL6. From Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-01-05
Question
Our lab want to know about to test anti-IL-6 antibody A00102-3 on human fibroblast for research purposes, then I may be interested in using anti-IL-6 antibody A00102-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Wu
Verified customer
Asked: 2015-04-02
Answer
The products we sell, including anti-IL-6 antibody A00102-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-02
