Anti-IL6 Antibody Picoband™

IL-6 antibody

Boster Bio Anti-IL6 Antibody Picoband™ catalog # A00102-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. Cited in 27 publication(s).

Product Info Summary

SKU: A00102-2
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-IL6 Antibody Picoband™

View all IL-6 Antibodies

SKU/Catalog Number

A00102-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL6 Antibody Picoband™ catalog # A00102-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00102-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence of rat IL6 (KTIQLILKALEEFLKVTMRSTRQT).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00102-2 is reactive to Il6 in Mouse, Rat

Applications

A00102-2 is guaranteed for ELISA, WB Boster Guarantee

Background of IL-6

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, the IL6 gene is mapped to chromosome 7p15.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Il6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20607

Gene ID

24498

Gene Name

Il6

Full Name

Interleukin-6

Weight

24.357kDa

Superfamily

IL-6 superfamily

Alternative Names

B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A Il6|ILg6, Ifnb2|interleukin 6|interleukin-6|IL-6|Interleukin 6 (interferon, beta 2)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il6, check out the Il6 Infographic

Il6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00102-2 has been cited in 27 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Driving GABAergic neurons optogenetically improves learning, reduces amyloid load and enhances autophagy in a mouse model of Alzheimer’s disease

Tan J,Luo J,Meng C,Jiang N,Cao J,Zhao J. Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway. Int Immunopharmacol.2020 Dec 11;90:107268.doi:10.1016/j. intimp.2020.107268.Epub ahead of print.PMID:333
Species: Rat
A00102-2 usage in article: APP:WB, SAMPLE:CEREBRAL CORTEX, DILUTION:NA

Potential Ameliorative Effects of Qing Ye Dan Against Cadmium Induced Prostatic Deficits via Regulating Nrf-2/HO-1 and TGF-%u03B21/Smad Pathways

Transfection with CXCR4 potentiates homing of mesenchymal stem cells in vitro and therapy of diabetic retinopathy in vivo

The expression and significance of IL-6, IFN-%u03B3, SM22%u03B1, and MMP-2 in rat model of aortic dissection

Distribution change of mast cells in human nasal polyps

The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway

Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm

Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats

Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication

The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells

Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-?B pathway

H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375

Ginsenoside Rg1 improves fertility and reduces ovarian pathological damages in premature ovarian failure model of mice

Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells

Angiotensin-(1-7) inhibits inflammation and oxidative stress to relieve lung injury induced by chronic intermittent hypoxia in rats

Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity

MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3

Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway

Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression

Zhao B, Gao WW, Liu YJ, Jiang M, Liu L, Yuan Q, Hou JB, Xia ZY. Neural Regen Res. 2017 Oct;12(10):1632-1639. doi: 10.4103/1673-5374.217337. The role of glycogen synthase kinase 3 beta in brain injury induced by myocardial ischemia/reperfusion inju...

Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.

Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.

Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...

Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.

Zhu J, Mu X, Zeng J, Xu C, Liu J, Zhang M, Li C, Chen J, Li T, Wang Y. Plos One. 2014 Jun 30;9(6):E101291. Doi: 10.1371/Journal.Pone.0101291. Ecollection 2014. Ginsenoside Rg1 Prevents Cognitive Impairment And Hippocampus Senescence In A Rat Model...

Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.

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16 Customer Q&As for Anti-IL6 Antibody Picoband™

Question

I would like using your anti-IL6 antibody for response to mechanical stimulus studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

We appreciate your inquiry. This A00102-2 anti-IL6 antibody is tested on rat heart tissue, tissue lysate, mouse heart tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-20

Question

I have attached the WB image, lot number and protocol we used for fibroblast using anti-IL6 antibody A00102-2. Please let me know if you require anything else.

J. Evans

Verified customer

Asked: 2020-02-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-05

Question

Do you have a BSA free version of anti-IL6 antibody A00102-2 available?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL6 antibody A00102-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-10

Question

We bought anti-IL6 antibody for WB on left coronary artery in a previous experiment. I am using rat, and I plan to use the antibody for ELISA next. My question regards examining left coronary artery as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

I have checked the website and datasheets of our anti-IL6 antibody and it appears that A00102-2 has been validated on rat in both WB and ELISA. Thus A00102-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-12

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-IL6 antibody A00102-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-08

Question

Will A00102-2 anti-IL6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

You can see on the product datasheet, A00102-2 anti-IL6 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-10

Question

We have seen staining in rat lung. What should we do? Is anti-IL6 antibody supposed to stain lung positively?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

Based on literature lung does express IL6. Based on Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-15

Question

I see that the anti-IL6 antibody A00102-2 works with WB, what is the protocol used to produce the result images on the product page?

D. Zhang

Verified customer

Asked: 2019-02-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-26

Question

My question regarding product A00102-2, anti-IL6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-12-26

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00102-2 anti-IL6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-26

Question

Is this A00102-2 anti-IL6 antibody reactive to the isotypes of IL6?

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

The immunogen of A00102-2 anti-IL6 antibody is A synthetic peptide corresponding to a sequence of rat IL6 (KTIQLILKALEEFLKVTMRSTRQT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-12

Question

Our lab were well pleased with the WB result of your anti-IL6 antibody. However we have seen positive staining in fibroblast secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-09-25

Answer

From literature, fibroblast does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-09-25

Question

I am interested in to test anti-IL6 antibody A00102-2 on rat fibroblast for research purposes, then I may be interested in using anti-IL6 antibody A00102-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-05-12

Answer

The products we sell, including anti-IL6 antibody A00102-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-05-12

Question

I was wanting to use your anti-IL6 antibody for WB for rat fibroblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fibroblast identification?

J. Wu

Verified customer

Asked: 2015-01-15

Answer

It shows on the product datasheet, A00102-2 anti-IL6 antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fibroblast in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-01-15

Question

Does anti-IL6 antibody A00102-2 work for WB with fibroblast?

J. Moore

Verified customer

Asked: 2014-01-14

Answer

According to the expression profile of fibroblast, IL6 is highly expressed in fibroblast. So, it is likely that anti-IL6 antibody A00102-2 will work for WB with fibroblast.

Boster Scientific Support

Answered: 2014-01-14

Question

We are currently using anti-IL6 antibody A00102-2 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

K. Gonzalez

Verified customer

Asked: 2013-08-27

Answer

The anti-IL6 antibody (A00102-2) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-08-27

Question

Is a blocking peptide available for product anti-IL6 antibody (A00102-2)?

Z. Krishna

Verified customer

Asked: 2013-05-14

Answer

We do provide the blocking peptide for product anti-IL6 antibody (A00102-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-05-14

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00102-2

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A00102-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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