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- Table of Contents
Il6 antibody (A00102-2) performs well in WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Western blot analysis of IL6 using anti-IL6 antibody (A00102-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse Ana-1 whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates,
Lane 5: mouse EL-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # A00102-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse Ana-1 whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates,
Lane 5: mouse EL-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # A00102-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
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Anti-IL6 Antibody Picoband®
Il6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | WB |
Tested Applications
Il6 antibody was positively detected by WB in:
rat spleen tissue, mouse spleen tissue, mouse Ana-1 whole cell, mouse RAW2647 whole cell, mouse EL-4 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IL6 Antibody Picoband®
View all IL6 Antibodies
View all IL6 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6, which shares 87.5% amino acid (aa) sequence identity with mouse IL6.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
24.4 kDa
Background of Il6
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, the IL6 gene is mapped to chromosome 7p15.
Cite This Product
Anti-IL6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00102-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
88%
Moderate Homology
Dog
46%
Low homology
Human
42%
Low homology
Cow
42%
Low homology
Monkey
42%
Low homology
Rabbit
38%
Low homology
Sheep
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Il6 antibody
Publications
Application:
Species:
Oral bovine osteopontin alters intestinal immune marker profiles in juvenile rats: concurrent changes in the aryl hydrocarbon receptor/signal transducer and activator of transcription 3/interleukin-6 axis
Published: 2026-07-10
Journal: Food Bioscience
Application: IF
Impact Factor: 6.20
Species: Rat
Combined treatment with Acorus tatarinowii Schott and Panax notoginseng saponins ameliorates brain–gut axis dysfunction in MCAO/R rats with suppression of TLR4/MyD88/NF-κB signaling and associated gut microbiota changes
Published: 2026-06-29
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.40
Species: Rat
PMID: 42445253
Resveratrol suppresses GATA4/Sortilin pathway to attenuate vascular smooth muscle cell senescence-associated secretory phenotype and vascular aging-related atherosclerosis
Published: 2026-06-16
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 4.70
Species: Rat
PMID: 42303194
Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells
Published: 2025-11-29
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.50
Species: Mouse
PMID: 41325790
Low-Frequency (1 Hz) Repetitive Trans-Spinal Magnetic Stimulation Attenuates NLRP3-Driven Neuroinflammation and Enhances Motor Recovery in Spinal Cord-Injured Mice
Published: 2025-08-18
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.10
Species: Mouse
PMID: 40873779
Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Published: 2025-08-18
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.10
Species: Rat
PMID: 40860947
Driving GABAergic neurons optogenetically improves learning, reduces amyloid load and enhances autophagy in a mouse model of Alzheimer’s disease
Published: 2020-03-12
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.99
Species: Mouse
PMID: 32173530
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Product Promise
We promise Anti-IL6 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00102-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026