Anti-Interleukin-6 IL6 Antibody Picoband®

Western blot analysis of IL6 using anti-IL6 antibody (PA1352).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant rat IL6 protein 5 ng.
Lane 2: recombinant rat IL6 protein 10 ng.
Lane 3: recombinant rat IL6 protein 0.5 ng.
Lane 4: recombinant rat IL6 protein 0.1 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1352) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL6 at approximately 22 kDa.

Western blot analysis of IL6 using anti-IL6 antibody (PA1352).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1352) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL6 at approximately 28-30 kDa. The expected band size for IL6 is at 24 kDa.

Western blot analysis of IL6 using anti-IL6 antibody (PA1352).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1352) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL6 at approximately 28-30 kDa. The expected band size for IL6 is at 24 kDa.

Western blot analysis of IL6 using anti-IL6 antibody (PA1352).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: 2h drug treated-human MCF-7 whole cell lysates,
Lane 3: 4h drug treated-human MCF-7 whole cell lysates,
Lane 4: 12h drug treated-human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1352) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:2000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system system. A specific band was detected for IL6 at 24 kDa. The expected band size for IL6 is at 24 kDa.

IHC analysis of IL6 using anti-IL6 antibody (PA1352).
IL6 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL6 Antibody (PA1352) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Histological evaluation and statistical analysis of diabetic wound after Mn3O4 treatment by immunohistochemical staining. Immunohistochemical staining of (A) IL-6, (B) CD86 and (C) CD206 in the wound bed at day 7. (D–F) Statistical results of immunohistochemical staining in each group. Data are presented as mean ± SD. Statistical significance was determined using t-test (n = 3, ns = no significance, ***P < 0.001, ****P < 0.0001).
Index in Regenerative Biomaterials under a CC BY license. DOI: 10.1093/rb/rbaf089

Collagen deposition, inflammation, and angiogenesis analyses in acute large-area wounds. (A) Masson staining and immunofluorescence of IL-6 (red) and CD31 (red) and a-SMA (green) in wound samples. Scale bars: 100 μm (top) and 50 μm (middle and bottom). (B to D) Quantification assessment of (B) collagen deposition, (C) IL-6 expression, and (D) CD31 expression. **P < 0.01, ***P < 0.001.
Index in PubMed under a CC BY license. PMID: 39109247

Relative expression of IL-6, caspase-3, and cleaved-caspase-3. (A) Immunoblot results of IL-6, caspase-3, and cleaved-caspase-3. (B) Analysis of IL-6 relative expression. One-way ANOVA showed differences among the five groups, F = 10.86, p < 0.0001. (C) Analysis of caspase-3 relative expression. One-way ANOVA showed differences among the five groups, F = 8.50, p = 0.0004. (D) Analysis of cleaved-caspase-3 relative expression. One-way ANOVA showed differences among the five groups, F = 17.36, p < 0.0001. ANOVA, analysis of variance; caspase-3, cysteinyl aspartate specific proteinase 3; IL-6, interleukin 6.
Index in PubMed under a CC BY license. PMID: 34975719

Experimental workflow. One hundred four rats were randomly divided into five groups: group S (sham, n = 20), group M (middle cerebral artery occlusion [MCAO], n = 28), group H2M (intermittent hypobaric hypoxia preconditioned MCAO group, 2 h/day, n = 20), group H6M (intermittent hypobaric hypoxia preconditioned MCAO group, 6 h/day, n = 28), and group HpM (persistent hypobaric hypoxia preconditioned MCAO group, n = 28). Behavioral tests and morphological staining (TTC staining) were used to analyze the severity of infarction. Total protein expression of NeuN (a specific marker of mature neurons), caspase-3, cleaved-caspase-3, and IL-6 was estimated using western blotting, which explained the severity of injury from different perspectives. Ultrastructural changes were observed under a transmission electron microscope. The most effective pretreatment group was selected for further label-free proteomic study and provided a reliable direction for mechanism exploration. Western blotting was used to verify the expression of the target protein, and key markers for the biological process were detected using immunofluorescence. caspase-3, cysteinyl aspartate specific proteinase 3; IL-6, interleukin 6; NeuN, neuron-specific nuclear protein; TTC, 2,3,5-triphenyl tetrazolium chloride.
Index in PubMed under a CC BY license. PMID: 34975719

Inhibitive effect of nanofiber membranes on anti-inflammation in Il-1β induced chondrocytes. a Relative mRNA expression levels of Il6, Tnf-a, iNos, Mmp1, Mmp3, and Mmp13 in IL-1β induced chondrocytes cultured on P, PGF, PF, PPF, or PPGF nanofiber membranes. b Protein expression of MMP13, TNF-a, and IL6 in IL-1β induced chondrocytes cultured on P, PGF, PF, PPF, or PPGF nanofiber membranes (Scale bars, 100 µm). The values were presented as mean ± SD ( n = 3; statistics: one-way ANOVA; # means p < 0.01, ** , ## means p < 0.01, *** , ### means p < 0.001, * is the statistical difference compare with P group and # is the statistical difference between the pairwise comparison).
Index in PubMed under a CC BY license. PMID: 36323709

MT inhibited the production of pro-inflammatory proteins in sleep-deprived rats. (A) Western blot bands showing the protein expression levels of IL-1β, IL-6, TNF-α, iNOS, and COX2 in the HP, respectively. (B–F) Relative protein expression level of IL-1β, IL-6, TNF-α, iNOS, and COX2 in the HP, respectively. The data are expressed as the means ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001 vs Control group; * p < 0.05, ** p < 0.01 vs. Model group.
Index in PubMed under a CC BY license. PMID: 39101143

Therapeutic effectiveness evaluated after administering AE@SiO 2 -MTX. (A) Knee joints were stained with HE and safranin O. Scale bar: 100 μm. (B) TNF-α, IL-6, MMP3 and MMP13 and DAPI staining applied to knee sections. (C) Line profile analysis verified the co-expression and co-localization of TNF-α and IL-6, MMP3 and MMP13. Scale bar: 50 μm.
Index in PubMed under a CC BY license. PMID: 40521182

Intravenous administration of AE@SiO 2 -MTX alleviated arthritis symptoms in CIA mice. (A) Visual outline of the in vivo treatment procedure. (B-D). Quantification of paw thickness, paw volume and arthritis score at multiple intervals after treatments. (E) Illustrative images of hindlimbs from each group before and after treatments. (F) Micro-CT images in 3D of fore paws, hind paws, and knee joints for different treatment groups. (G-H) The evaluation of BMD levels in six groups post-treatments. (I-J) Plasma levels of IL-6 and IL-10 after treatments. Data were expressed as mean±SD. Data were analyzed for statistical significance via One-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Index in PubMed under a CC BY license. PMID: 40521182

PF-127/hADSCs-Exos complex treatment inhibits inflammatory reaction. a Representative images of TNF-α immunostaining at 4, 7, and 10 days after treatment. Scale bar = 20 µm. b Quantification of TNF-α + IHC stained tissues. c Representative images illustrating IHC results of IL-6 at 4, 7, and 10 days after surgery. Scale bar = 20 µm. d Quantification of IL-6 + IHC stained tissues. e IHC images of wound sections stained with CD68 on days 4, 7, and 10 post-wounding. Scale bar = 20 µm. f Quantification of the number of CD68 positive cells in the wound area on days 4, 7, and 10. g IHC images of wound sections stained with CD206 at days 4, 7, and 10 post-wounding. Scale bar = 20 µm. h Quantification of the number of CD206 positive cells in the wound area on days 4, 7, and 10. In b, d, and f , data are shown as mean ± SEM; n = 6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus vehicle control group
Index in PubMed under a CC BY license. PMID: 35941707