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Il6 antibody (PA1352) performs well in IHC, WB and shows reactivity with Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 106 Publications

pa1352 il6 primary antibodies ihc testing 1

IHC analysis of IL6 using anti-IL6 antibody (PA1352).
IL6 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL6 Antibody (PA1352) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Interleukin-6 IL6 Antibody Picoband®

Name: Anti-Interleukin-6 IL6 Antibody Picoband®
Brand: Boster Bio
SKU: PA1352
Price: 370 USD
Availability: InStock
Rating: 90 (2 reviews)

Il6 antibody

106 Publications|

2 Reviews

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Reactive Species	Host	Application
Mouse, Rat	Rabbit	IHC, WB

Tested Applications

Il6 antibody was positively detected by WB in:

recombinant rat IL6 protein, mouse spleen tissue, rat thymus tissue, rat spleen tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Il6 antibody was positively detected by IHC in:

rat spleen tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Mouse

Tested

Rat

Tested

Recommend

Product Information

Product Name

Anti-Interleukin-6 IL6 Antibody Picoband®
View all IL6 Antibodies

Form

Lyophilized

Uniprot

P20607

Gene ID

24498

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6, different from the related mouse sequence by two amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

28-30 kDa

Calculated Molecular Weight

24.4 kDa

Background of Il6

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL6 gene to chromosome 7p15.

Cite This Product

Anti-Interleukin-6 IL6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1352)

Protocols

Western Blot protocol for Il6 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Il6 antibody

Review Protocol

Publications

Dual modulation of lipid-lowering and anti-inflammatory effects of Thymus quinquecostatus Celak. ethanol extract on atherosclerosis: integration of UPLC–Q–TOF–MS/MS, in silico, in vivo, and in vitro studies

Published: 2026-08-31

Journal: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

Impact Factor: 4.00

PMID: 42675565

SKU	PA1352
Application	Western Blot
Sample	PC-12 (rat adrenal pheochromocytoma cells, highly differentiated)
Sample Processing Description	① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	Interleukin-6 IL6 Antibody Picoband®
Primary Incubation	1:2500, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. Experimental results showed clear target bands. After modeling, the total expression level of MAPK14 protein was upregulated, and the expression level decreased after drug treatment, which was in line with expectations.

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Anti-Interleukin-6 IL6 Antibody Picoband®

Il6 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Dual modulation of lipid-lowering and anti-inflammatory effects of Thymus quinquecostatus Celak. ethanol extract on atherosclerosis: integration of UPLC–Q–TOF–MS/MS, in silico, in vivo, and in vitro studies

Suppression of Ciliogenesis Alleviates Cellular Senescence via AKT Signaling in Gingival Aging

Artemin Serves as a Novel Predictive Biomarker and Exerts a Protective Effect Against Preterm Birth

Multi-omics profiling reveals systemic rejuvenation of the aged kidney through senolytic therapy

Buyiniuxi pill promotes hair regrowth in a DHT-induced mouse model of androgenetic alopecia

Neuregulin 4 improves white adipose tissue inflammation in mice with polycystic ovary syndrome

Bioadhesive Hydrogel With Polyphenol-Armored Nanogene Rejuvenates Chondrocyte Senescence for Aged Osteoarthritis Therapy

Colchicine alleviates severe acute pancreatitis in rats by inhibiting acinar cell ferroptosis via LCN2/MAPK/ERK signaling axis

Freeze-Drying Tumor Tissues Derived Bio-Patches With Hair Melanin Nanoparticles Integration for Wound Healing

Glucose/ROS-responsive and redox-gated adaptive hydrogel dressing for accelerating diabetic wound repair via synergistic cGAS/STING pathway inhibition and oxidative stress alleviation

Engineering Goat Milk-Derived Extracellular Vesicles for Dual-Model Imaging and Anti-Inflammatory Photothermal Therapy of Pancreatic Cancer

Artificial neural network-based analysis of ferroptosis-associated molecular subtypes and immunological profiles in abdominal aortic aneurysm

Perfluorohexane/hemoglobin nano-oxygen carriers enhance transplanted islet graft survival via hypoxia alleviation and mitochondrial repair

Di-n-pentyl phthalate exposure alters intestinal structure and gut microbiota composition and characteristics in mice

Sleep deprivation attenuates acute ischemic stroke-induced hippocampal neuronal injury via the IL-38/NF-κB axis

Multifunctional copper-based metal-organic frameworks/chitosan derivative/exosome composite sponge for skin healing

In vivo anti-inflammatory, analgesic activities and phytochemical profile of Hu Lisan ethanol extract

Ustiloxins induce kidney injury via the TLR2/MAPK/NF-κB pathway

Vancomycin nanocomposite-functionalized M2 macrophages for precision targeting of MRSA in prosthetic joint infections

Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays

Tanshinone IIA and hydroxy safflower yellow A reduce cerebral injury via TLR4/NF-κB pathway in rats

Tobacco ethanol extract accelerates wound healing through anti-inflammation and promoting angiogenesis

Injured renal cell membrane encapsulated ICG/CORM-401 for homologous targeted NIR theranostics of acute kidney injury

ROS Scavenging Mn3O4 nanozyme regulated immune microenvironment and affects intercellular interaction to promote wound healing in diabetes

Bioinspired exosome-SiO2 nanohybrid therapeutic for rheumatoid arthritis treatment

High fluid shear stress induces Hippo/YAP pathway in articular cartilage superficial layer cells: A potential mechanistic link to osteoarthritis

Programmed Drug Delivering Janus Hydrogel Adapted to the Spatio-temporal Therapeutic Window for Achilles Tendon Repair

Histone lactylation-derived prelamin A accelerates pulmonary arterial smooth muscle cells senescence in hypoxia-induced pulmonary hypertension rats

Synergistic therapeutic effect of parecoxib and ilomastat combination in osteoarthritis via inhibition of IL-17/PI3K/AKT/NF-κB activity

Enhanced activation of retinal progenitor cells by zinc silicate bioceramics for effective functional restoration in retinal degenerative diseases

Investigating the molecular mechanism of epimedium herb in treating rheumatoid arthritis through network pharmacology, molecular docking, and experimental validation

Exploring the mechanism of Taohong Siwu Decoction in treating ischemic stroke injury via the circDnajc1/miR-27a-5p/C1qc signaling axis

Semaglutide Alleviates Ovary Inflammation via the AMPK/SIRT1/NF‑κB Signaling Pathway in Polycystic Ovary Syndrome Mice

Decellularized Tumor Tissues Integrated with Polydopamine for Wound Healing

Intestinal Akkermansia muciniphila is Beneficial to Functional Recovery Following Ischemic Stroke

Gabapentin alleviates peripheral nerve sensitization induced by inflammatory arthritis via ionotropic glutamate receptor NR2B subunit

Porcine reproductive and respiratory syndrome virus infects the reproductive system of male piglets and impairs development of the blood–testis barrier

Comparison of CUMS at different pregnancy stages, maternal separation, and their effects on offspring in postpartum depression mouse models

Melatonin modulates TLR4/MyD88/NF-κB signaling pathway to ameliorate cognitive impairment in sleep-deprived rats

Dual-Drug Nanomedicine Assembly with Synergistic Anti-Aneurysmal Effects via Inflammation Suppression and Extracellular Matrix Stabilization

Graphene oxide quantum dots-loaded sinomenine hydrochloride nanocomplexes for effective treatment of rheumatoid arthritis via inducing macrophage repolarization and arresting abnormal proliferation of fibroblast-like synoviocytes

“Butterfly-effect” nano-platforms for cascade immune amplification and tumor clearance through multi-modal therapy and immune sensitization

Mechanism of Salvia miltiorrhiza Bunge extract to alleviate Chronic Sleep Deprivation-Induced cognitive dysfunction in rats

Acacetin inhibits activation of microglia to improve neuroinflammation after subarachnoid hemorrhage through the PERK signaling pathway mediated autophagy

Depletion of ApoA5 aggravates spontaneous and diet-induced nonalcoholic fatty liver disease by reducing hepatic NR1D1 in hamsters

Betulinic acid alleviates neuropathic pain induced by chronic constriction injury of the sciatic nerve in mice

High drug loading hydrophobic cross-linked dextran microspheres as novel drug delivery systems for the treatment of osteoarthritis

Sirt4 deficiency promotes the development of atherosclerosis by activating the NF-κB/IκB/CXCL2/3 pathway

A fresh pH-responsive imipenem-loaded nanocarrier against Acinetobacter baumannii with a synergetic effect

Reversing inflammatory microenvironment by a single intra-articular injection of multi-stimulus responsive lipogel to relieve rheumatoid arthritis and promote joint repair

Mangiferin alleviates renal inflammatory injury in spontaneously hypertensive rats by inhibiting MCP-1/CCR2 signaling pathway

Rapidly Inhibiting the Inflammatory Cytokine Storms and Restoring Cellular Homeostasis to Alleviate Sepsis by Blocking Pyroptosis and Mitochondrial Apoptosis Pathways

Cannabidiol-Loaded Poly Lactic-Co-Glycolic Acid Nanoparticles with Improved Bioavailability as a Potential for Osteoarthritis Therapeutic

Protein kinase D1 promotes the survival of random-pattern skin flaps in rats

Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

ROS-responsive PPGF nanofiber membrane as a drug delivery system for long-term drug release in attenuation of osteoarthritis

Adipose-Derived Stem Cell Exosomes Inhibit Hypertrophic Scaring Formation by Regulating Th17/Treg Cell Balance

Antibacterial, adhesive, and MSC exosomes encapsulated microneedles with spatio-temporal variation functions for diabetic wound healing

Reduction of natural killer cells is associated with poor outcomes in patients with hepatitis B virus-related acute-on-chronic liver failure

Hyaluronic acid-g-lipoic acid granular gel for promoting diabetic wound healing

Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora

Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration

NIR-driven polydopamine-based nanoenzymes as ROS scavengers to suppress osteoarthritis progression

Efficient starvation therapy with three-pathway blocking in combination with PTT/CDT for TME reversal and tumor apoptosis

Using extracellular vesicles derived from human umbilical cord mesenchymal stem cells for a topical coating promotes oral mucositis healing in rats

Preconditioning With Intermittent Hypobaric Hypoxia Attenuates Stroke Damage and Modulates Endocytosis in Residual Neurons

Reactive oxygen species (ROS)-responsive nanoprobe for bioimaging and targeting therapy of osteoarthritis