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Product Info Summary
| SKU: | M00918 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-IL12A Mouse Monoclonal Antibody [Clone ID: OTI2A8]
SKU/Catalog Number
M00918
Size
100 µl
Description
Boster Bio IL12A mouse monoclonal antibody, clone OTI2A8 (formerly 2A8). Catalog# M00918. Tested in WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IL12A Mouse Monoclonal Antibody [Clone ID: OTI2A8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00918)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2A8
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 57-253 of human IL12A (NP_000873) produced in E.coli.
Reactive Species
M00918 is reactive to IL12A in Human
Calculated molecular weight
24.9 kDa
Background of IL12A
Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00918 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500
Validation Images & Assay Conditions
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Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid, lane 1) , human IL12A plasmid, lane 2), mouse IL12A plasmid, lane 3) , rat IL12A plasmid, lane 4) using antiIL12A antibody M00918 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)
Specific Publications For Anti-IL12A Mouse Monoclonal Antibody [Clone ID: OTI2A8] (M00918)
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