Mouse IL-12(p70) ELISA Kit PicoKine™

Mouse IL-12 (p70) ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il12a in cell culture supernatants and serum. Sensitivity: 1.5pg/ml. Cited in 25 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK0422
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse IL-12(p70) ELISA Kit PicoKine™

See all IL12A products

SKU/Catalog Number

EK0422

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-12 (p70) ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il12a in cell culture supernatants and serum. Sensitivity: 1.5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IL-12(p70) ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0422)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: (M23-S335)+ (R23-A215)

Sensitivity

<1.5 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0422 is reactive to Il12a in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0422 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0422-CAP Anti-Mouse Il12a Pre-coated 96-well strip microplate 1
EK0422-ST Mouse Il12a Standard 2 vials, 10 ng/tube
EK0422-DA Mouse Il12a Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0410.1010.1590.2700.4590.8691.5072.292

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-12(p70) ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3715041033147442
Standard deviation2.189.1530.752.448.9638.45
CV (%)7.5%6.1%7.5%7.4%6.1%8.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL12A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 137383940381.112.9%
Sample 21501511711681609.565.9%
Sample 341041644241742112.272.9%
*number of samples for each test n=16.

Gene/Protein Information For Il12a (Source: Uniprot.Org, NCBI)

Uniprot ID

P29459

Gene ID

3592

Gene Name

Il12a

Full Name

Interleukin-12 subunit alpha

Weight

24.874kDa

Superfamily

IL-6 superfamily

Alternative Names

CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35 IL12A CLMF, IL-12A, NFSK, NKSF1, P35 interleukin 12A interleukin-12 subunit alpha|CLMF p35|IL-12, subunit p35|IL35 subunit|NF cell stimulatory factor chain 1|NK cell stimulatory factor chain 1|cytotoxic lymphocyte maturation factor 1, p35|cytotoxic lymphocyte maturation factor 35 kDa subunit|interleukin 12, p35|interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)|interleukin-12 alpha chain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il12a, check out the Il12a Infographic

Il12a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il12a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0422 has been cited in 25 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Autophagy inhibitor enhance ZnPc/BSA nanoparticle induced photodynamic therapy by suppressing PD-L1 expression in osteosarcoma immunotherapy
Species: Mouse

Laser Immunotherapy in Combination with Perdurable PD-1 Blocking for the Treatment of Metastatic Tumors
Species: Mouse

Clara cell 10-kDa protein inhibits TH17 responses through modulating dendritic cells in the setting of allergic rhinitis
Species: Mouse

Ultrasmall Graphene Oxide Supported Gold Nanoparticles as Adjuvants Improve Humoral and Cellular Immunity in Mice
Species: Mouse

Lentiviral-mediated shRNA against RelB induces the generation of tolerogenic dendritic cells

The upregulation of integrin ?D?2 (CD11d/CD18) on inflammatory macrophages promotes macrophage retention in vascular lesions and development of atherosclerosis

H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375

MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3

4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-?B signaling in a ROS independent manner

Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection

Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell-cell interactions

Stimulation of dendritic cells by DAMPs in ALA-PDT treated SCC tumor cells

Hybrid phage displaying SLAQVKYTSASSI induces protection against?Candida albicans?challenge in BALB/c mice

The dendritic cell mannose receptor mediates allergen internalization and maturation involving notch 1 signalling

Wang W, Liu M, Wang Y, Yang T, Li D, Ding F, Sun H, Bai G, Li Q. Evid Based Complement Alternat Med. 2018 Jan 28;2018:2305683. doi: 10.1155/2018/2305683. eCollection 2018. Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via No...

Huang Z, Zhang Z, Jiang Y, Zhang D, Chen J, Dong L, Zhang J. J Control Release. 2012 Mar 10;158(2):286-92. Doi: 10.1016/J.Jconrel.2011.11.013. Epub 2011 Nov 18. Targeted Delivery Of Oligonucleotides Into Tumor-Associated Macrophages For Cancer Imm...

Huang Z, Yang Y, Jiang Y, Shao J, Sun X, Chen J, Dong L, Zhang J. Biomaterials. 2013 Jan;34(3):746-55. Doi: 10.1016/J.Biomaterials.2012.09.062. Epub 2012 Oct 27. Anti-Tumor Immune Responses Of Tumor-Associated Macrophages Via Toll-Like Receptor 4 ...

Gui J, Xiong F, Yang W, Li J, Huang G. Am J Reprod Immunol. 2012 May;67(5):383-90. Doi: 10.1111/J.1600-0897.2011.01097.X. Epub 2012 Jan 9. Effects Of Acupuncture On Lif And Il-12 In Rats Of Implantation Failure.

Li Y, Li X, Liu H, Zhuang S, Yang J, Zhang F. Oncol Lett. 2014 Feb;7(2):576-582. Epub 2013 Dec 9. Intranasal Immunization With Recombinant Lactococci Carrying Human Papillomavirus E7 Protein And Mouse Interleukin-12 Dna Induces E7-Specific Antitum...

Hara T, Mimura K, Abe T, Shioi G, Seiki M, Sakamoto T. J Biol Chem. 2011 Sep 16;286(37):32542-51. Doi: 10.1074/Jbc.M111.271726. Epub 2011 Jul 21. Deletion Of The Mint3/Apba3 Gene In Mice Abrogates Macrophage Functions And Increases Resistance To L...

Ma Yj, He M, Han Ja, Yang L, Ji Xy. Scand J Immunol. 2013 Oct;78(4):387-93. Doi: 10.1111/Sji.12097. A Clinical Study Of Hbsag-Activated Dendritic Cells And Cytokine-Induced Killer Cells During The Treatment For Chronic Hepatitis B.

Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...

Li J, Li Ff, Zuo W, Zhou Y, Hao Hy, Dang J, Jiang M, He Mz, Deng Dr. J Huazhong Univ Sci Technolog Med Sci. 2014 Aug;34(4):586-90. Doi: 10.1007/S11596-014-1320-2. Epub 2014 Aug 19. Up-Regulated Expression Of Tim-3/Gal-9 At Maternal-Fetal Interface...

Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????

Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.

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1 Reviews For Mouse IL-12(p70) ELISA Kit PicoKine™

0

"Good Mouse IL-12 ELISA Kit"

Excellent

Source: Biocompare.com

"Good Mouse IL-12 ELISA Kit"

--Daisy Dai University of Miami Surgery Lab Director / Manager

Application ELISA
Starting Material Crispr MEK and STAT3 KO CAFs
Tips This kit doesn't provide PC

"We investigate how alterations in RAS and STAT3 signaling pathways affect the tumor stroma and immune microenvironment in pancreatic cancer. This kit was used to detect the IL-12 level in CM from Crispr MEK and STAT3 KO CAFs. The whole process was about 7 hours including sample preparation."

Verified customer

Submitted

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4 Customer Q&As for Mouse IL-12(p70) ELISA Kit PicoKine™

Question

Q: how to thaw whole blood sample for IL12A ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-09-11

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL12A for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-09-11

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-05-21

Question

Q: Can IL12A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-06-25

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-06-25

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL12A in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-04-03

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL12A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-04-03

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