Product Info Summary
SKU: | A02628-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Involucrin IVL Antibody
View all Involucrin Antibodies
SKU/Catalog Number
A02628-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Involucrin IVL Antibody catalog # A02628-1. Tested in WB,IHC,Flow Cytometry applications. This antibody reacts with Human,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Involucrin IVL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02628-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with full length myosin-6 construct expressed in SF9 insect cells corresponding to porcine myosyn-6 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02628-1 is reactive to IVL in Human, Rat
Applications
A02628-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
68.479kDa
Background of Involucrin
Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of IVL using Anti-Involucrin IVL Antibody (A02628-1).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Involucrin antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Involucrin IVL Antibody A02628-1
Western blot analysis of Involucrin on human skin tissue lysate using Involucrin antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For IVL (Source: Uniprot.org, NCBI)
Gene Name
IVL
Full Name
Involucrin
Weight
68.479kDa
Superfamily
involucrin family
Alternative Names
involucrin IVL involucrin involucrin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IVL, check out the IVL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IVL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Involucrin IVL Antibody (A02628-1)
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