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Product Info Summary
| SKU: | M00115 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IRF7 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00115
BM4484 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRF7 Rabbit Monoclonal Antibody catalog # M00115. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00115)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FAH-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRF7
Reactive Species
M00115 is reactive to IRF7 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00115 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
IHC: rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of IRF7 using anti-IRF7 antibody (M00115).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF7 antigen affinity purified monoclonal antibody (M00115) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRF7 at approximately 54 kDa. The expected band size for IRF7 is at 54 kDa.
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Immunohistochemical analysis of paraffin-embedded human pancreas, using IRF7 Antibody.
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Immunoprecipitation analysis of IRF7 using anti-IRF7 (M00115).
Mouse brain lysates were prepared in RIPA with protease inhibitors. Anti-IRF7 (M00115) was pre-bound to Protein A+G magnetic beads and incubated with the lysates; a normal rabbit IgG IP ran in parallel. Beads were washed with TBST and the bound proteins were eluted with SDS sample buffer by heating.
Proteins were separated on a 10% SDS-PAGE (80 V stacking / 120 V resolving) and transferred to NC membrane (≈150 mA, 50-90 min). Membranes were blocked with 5% milk/TBST, probed with M00115 (o/n, 4 °C), then with HRP anti-rabbit light-chain IgG (BM2007), and developed by ECL.
Lane 1: Input (10% of starting lysate),
Lane 2: Control IgG IP,
Lane 3: IRF7 IP (M00115).
A ~54 kDa band corresponding to IRF7 was detected in the IRF7 IP and positive-control lanes, while the IgG control showed no specific band.
Specific Publications For Anti-IRF7 Rabbit Monoclonal Antibody (M00115)
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17 Customer Q&As for Anti-IRF7 Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-IRF7 Rabbit Monoclonal antibody. However we have seen positive staining in spleen nucleus. cytoplasm. note=the phosphorylated using this antibody. Is that expected? Could you tell me where is IRF7 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From literature, spleen does express IRF7. Generally IRF7 expresses in nucleus. cytoplasm. note=the phosphorylated. Regarding which tissues have IRF7 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 9315633
Boster Scientific Support
Answered: 2020-04-28
Question
I was wanting to use your anti-IRF7 Rabbit Monoclonal antibody for IP for human right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lobe of liver identification?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
As indicated on the product datasheet, M00115 anti-IRF7 Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-21
Question
I see that the anti-IRF7 Rabbit Monoclonal antibody M00115 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-13
Question
Is a blocking peptide available for product anti-IRF7 Rabbit Monoclonal antibody (M00115)?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
We do provide the blocking peptide for product anti-IRF7 Rabbit Monoclonal antibody (M00115). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-05
Question
Does anti-IRF7 Rabbit Monoclonal antibody M00115 work on primate WB with spleen?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Our lab technicians have not tested anti-IRF7 Rabbit Monoclonal antibody M00115 on primate. You can run a BLAST between primate and the immunogen sequence of anti-IRF7 Rabbit Monoclonal antibody M00115 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-24
Question
Can you help my question with product M00115, anti-IRF7 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00115 anti-IRF7 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-IRF7 Rabbit Monoclonal antibody M00115. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-29
Question
We have been able to see staining in rat right lobe of liver. Any tips? Is anti-IRF7 Rabbit Monoclonal antibody supposed to stain right lobe of liver positively?
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
From literature right lobe of liver does express IRF7. From Uniprot.org, IRF7 is expressed in right lobe of liver, spleen, among other tissues. Regarding which tissues have IRF7 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 9315633
Boster Scientific Support
Answered: 2019-04-16
Question
I am interested in to test anti-IRF7 Rabbit Monoclonal antibody M00115 on human right lobe of liver for research purposes, then I may be interested in using anti-IRF7 Rabbit Monoclonal antibody M00115 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
The products we sell, including anti-IRF7 Rabbit Monoclonal antibody M00115, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-16
Question
Is this M00115 anti-IRF7 Rabbit Monoclonal antibody reactive to the isotypes of IRF7?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
The immunogen of M00115 anti-IRF7 Rabbit Monoclonal antibody is A synthesized peptide derived from human IRF7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-08
Question
you antibody using your anti-IRF7 Rabbit Monoclonal antibody for regulation of monocyte differentiation studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
B. Mangal
Verified customer
Asked: 2017-01-27
Answer
We appreciate your inquiry. This M00115 anti-IRF7 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-01-27
Question
We are currently using anti-IRF7 Rabbit Monoclonal antibody M00115 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
B. Gonzalez
Verified customer
Asked: 2016-11-09
Answer
The anti-IRF7 Rabbit Monoclonal antibody (M00115) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-11-09
Question
See attached the WB image, lot number and protocol we used for right lobe of liver using anti-IRF7 Rabbit Monoclonal antibody M00115. Please let me know if you require anything else.
M. Wu
Verified customer
Asked: 2016-02-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-11
Question
Does anti-IRF7 Rabbit Monoclonal antibody M00115 work for IP with right lobe of liver?
N. Brown
Verified customer
Asked: 2015-12-25
Answer
According to the expression profile of right lobe of liver, IRF7 is highly expressed in right lobe of liver. So, it is likely that anti-IRF7 Rabbit Monoclonal antibody M00115 will work for IP with right lobe of liver.
Boster Scientific Support
Answered: 2015-12-25
Question
Is there a BSA free version of anti-IRF7 Rabbit Monoclonal antibody M00115 available?
M. Jones
Verified customer
Asked: 2015-09-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IRF7 Rabbit Monoclonal antibody M00115 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-10
Question
We ordered your anti-IRF7 Rabbit Monoclonal antibody for Flow Cytometry on spleen in a previous project. I am using mouse, and We intend to use the antibody for ICC next. I am looking for examining spleen as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
K. Singh
Verified customer
Asked: 2015-04-27
Answer
I have checked the website and datasheets of our anti-IRF7 Rabbit Monoclonal antibody and it appears that M00115 has been validated on mouse in both Flow Cytometry and ICC. Thus M00115 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-04-27
Question
Does M00115 anti-IRF7 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Jones
Verified customer
Asked: 2013-03-12
Answer
As indicated on the product datasheet, M00115 anti-IRF7 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-03-12
