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Product Info Summary
| SKU: | PB9429 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-VEGF Receptor 2/KDR Antibody Picoband®
SKU/Catalog Number
PB9429
PB0410 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGF Receptor 2/KDR Antibody Picoband® catalog # PB9429. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF Receptor 2/KDR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9429)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human KDR, different from the related mouse and rat sequences by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9429 is reactive to KDR in Human
Observed Molecular Weight
152 kDa
Calculated molecular weight
151.5 kDa
Background of KDR
KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al. (1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells (Plate et al., 1993). Flk1 is the mouse homolog of KDR (Matthews et al., 1991).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9429 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of KDR using anti-KDR antibody (PB9429).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDR antigen affinity purified polyclonal antibody (Catalog # PB9429) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDR at approximately 152 kDa. The expected band size for KDR is at 152 kDa.
Specific Publications For Anti-VEGF Receptor 2/KDR Antibody Picoband® (PB9429)
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Customer Q&As
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14 Customer Q&As for Anti-VEGF Receptor 2/KDR Antibody Picoband®
Question
Will PB9429 anti-VEGF Receptor 2/KDR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
It shows on the product datasheet, PB9429 anti-VEGF Receptor 2/KDR antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-26
Question
We are currently using anti-VEGF Receptor 2/KDR antibody PB9429 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The anti-VEGF Receptor 2/KDR antibody (PB9429) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
Is a blocking peptide available for product anti-VEGF Receptor 2/KDR antibody (PB9429)?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
We do provide the blocking peptide for product anti-VEGF Receptor 2/KDR antibody (PB9429). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-14
Question
We need to test anti-VEGF Receptor 2/KDR antibody PB9429 on human lung for research purposes, then I may be interested in using anti-VEGF Receptor 2/KDR antibody PB9429 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Gonzalez
Verified customer
Asked: 2020-01-02
Answer
The products we sell, including anti-VEGF Receptor 2/KDR antibody PB9429, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-02
Question
Will anti-VEGF Receptor 2/KDR antibody PB9429 work for WB with lung?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
According to the expression profile of lung, KDR is highly expressed in lung. So, it is likely that anti-VEGF Receptor 2/KDR antibody PB9429 will work for WB with lung.
Boster Scientific Support
Answered: 2019-11-06
Question
I have a question about product PB9429, anti-VEGF Receptor 2/KDR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9429 anti-VEGF Receptor 2/KDR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-13
Question
I see that the anti-VEGF Receptor 2/KDR antibody PB9429 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-11
Question
I was wanting to use your anti-VEGF Receptor 2/KDR antibody for WB for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
As indicated on the product datasheet, PB9429 anti-VEGF Receptor 2/KDR antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-12
Question
Do you have a BSA free version of anti-VEGF Receptor 2/KDR antibody PB9429 available?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-VEGF Receptor 2/KDR antibody PB9429 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-VEGF Receptor 2/KDR antibody PB9429. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-19
Question
I have attached the WB image, lot number and protocol we used for lung using anti-VEGF Receptor 2/KDR antibody PB9429. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-29
Question
Is this PB9429 anti-VEGF Receptor 2/KDR antibody reactive to the isotypes of KDR?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
The immunogen of PB9429 anti-VEGF Receptor 2/KDR antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human KDR (29-60aa LDLPRLSIQKDILTIKANTTLQITCRGQRDLD), different from the related mouse and rat sequences by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-27
Question
We have seen staining in human lung. Any tips? Is anti-VEGF Receptor 2/KDR antibody supposed to stain lung positively?
J. Carter
Verified customer
Asked: 2015-01-23
Answer
From literature lung does express KDR. From Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2015-01-23
Question
Our lab were content with the WB result of your anti-VEGF Receptor 2/KDR antibody. However we have observed positive staining in umbilical vein cell junction. endoplasmic using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?
R. Krishna
Verified customer
Asked: 2013-09-02
Answer
From literature, umbilical vein does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2013-09-02
