Anti-VEGF Receptor 2/KDR Antibody Picoband™

Boster Bio Anti-VEGF Receptor 2/KDR Antibody Picoband™ catalog # PB9429. Tested in WB applications. This antibody reacts with Human. Cited in 6 publication(s).

Product Info Summary

SKU: PB9429
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-VEGF Receptor 2/KDR Antibody Picoband™

See all VEGFR2/KDR/Flk-1 products

SKU/Catalog Number

PB9429

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VEGF Receptor 2/KDR Antibody Picoband™ catalog # PB9429. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VEGF Receptor 2/KDR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9429)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human KDR (29-60aa LDLPRLSIQKDILTIKANTTLQITCRGQRDLD), different from the related mouse and rat sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9429 is reactive to KDR in Human

Applications

PB9429 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For KDR (Source: Uniprot.Org, NCBI)

Uniprot ID

P35968

Gene ID

3791

Gene Name

KDR

Full Name

Vascular endothelial growth factor receptor 2

Weight

151.527kDa

Superfamily

protein kinase superfamily

Alternative Names

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KDR, check out the KDR Infographic

KDR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KDR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9429 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis

Zhu Y, Tuerxun A, Hui Y, Abliz P. Exp Ther Med. 2014 Aug;8(2):647-651. Epub 2014 Jun 12. Effects Of Propranolol And Isoproterenol On Infantile Hemangioma Endothelial Cells In Vitro.

Sun Y, Chen S, Song G, Ren L, Wei L, Liu N, Zhang D, Lv X. Int J Mol Med. 2012 Sep;30(3):622-8. Doi: 10.3892/Ijmm.2012.1032. Epub 2012 Jun 14. Effect Of Visfatin On The Function Of Endothelial Progenitor Cells In High-Fat-Fed Obese Rat...

Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1

Wang L, Liu X, Wang H, Wang S. Oncol Lett. 2013 Jul;6(1):175-180. Epub 2013 May 15. Correlation Of The Expression Of Vascular Endothelial Growth Factor And Its Receptors With Microvessel Density In Ovarian Cancer.

Wu Y, You H, Ma P, Li L, Yuan Y, Li J, Ye X, Liu X, Yao H, Chen R, Lai K, Yang X. Plos One. 2013 May 9;8(5):E62827. Doi: 10.1371/Journal.Pone.0062827. Print 2013. Role Of Transient Receptor Potential Ion Channels And Evoked Levels Of Neuropeptides...

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14 Customer Q&As for Anti-VEGF Receptor 2/KDR Antibody Picoband™

Question

Will PB9429 anti-VEGF Receptor 2/KDR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

It shows on the product datasheet, PB9429 anti-VEGF Receptor 2/KDR antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-26

Question

We are currently using anti-VEGF Receptor 2/KDR antibody PB9429 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

The anti-VEGF Receptor 2/KDR antibody (PB9429) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-10

Question

Is a blocking peptide available for product anti-VEGF Receptor 2/KDR antibody (PB9429)?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

We do provide the blocking peptide for product anti-VEGF Receptor 2/KDR antibody (PB9429). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-14

Question

We need to test anti-VEGF Receptor 2/KDR antibody PB9429 on human lung for research purposes, then I may be interested in using anti-VEGF Receptor 2/KDR antibody PB9429 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Gonzalez

Verified customer

Asked: 2020-01-02

Answer

The products we sell, including anti-VEGF Receptor 2/KDR antibody PB9429, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-02

Question

Will anti-VEGF Receptor 2/KDR antibody PB9429 work for WB with lung?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

According to the expression profile of lung, KDR is highly expressed in lung. So, it is likely that anti-VEGF Receptor 2/KDR antibody PB9429 will work for WB with lung.

Boster Scientific Support

Answered: 2019-11-06

Question

I have a question about product PB9429, anti-VEGF Receptor 2/KDR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9429 anti-VEGF Receptor 2/KDR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-13

Question

I see that the anti-VEGF Receptor 2/KDR antibody PB9429 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-11

Question

I was wanting to use your anti-VEGF Receptor 2/KDR antibody for WB for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?

Verified Customer

Verified customer

Asked: 2019-02-12

Answer

As indicated on the product datasheet, PB9429 anti-VEGF Receptor 2/KDR antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-12

Question

Do you have a BSA free version of anti-VEGF Receptor 2/KDR antibody PB9429 available?

Verified Customer

Verified customer

Asked: 2018-10-25

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-VEGF Receptor 2/KDR antibody PB9429 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-10-25

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-VEGF Receptor 2/KDR antibody PB9429. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-09-19

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-19

Question

I have attached the WB image, lot number and protocol we used for lung using anti-VEGF Receptor 2/KDR antibody PB9429. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-12-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-29

Question

Is this PB9429 anti-VEGF Receptor 2/KDR antibody reactive to the isotypes of KDR?

Verified Customer

Verified customer

Asked: 2017-11-27

Answer

The immunogen of PB9429 anti-VEGF Receptor 2/KDR antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human KDR (29-60aa LDLPRLSIQKDILTIKANTTLQITCRGQRDLD), different from the related mouse and rat sequences by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-27

Question

We have seen staining in human lung. Any tips? Is anti-VEGF Receptor 2/KDR antibody supposed to stain lung positively?

J. Carter

Verified customer

Asked: 2015-01-23

Answer

From literature lung does express KDR. From Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454

Boster Scientific Support

Answered: 2015-01-23

Question

Our lab were content with the WB result of your anti-VEGF Receptor 2/KDR antibody. However we have observed positive staining in umbilical vein cell junction. endoplasmic using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?

R. Krishna

Verified customer

Asked: 2013-09-02

Answer

From literature, umbilical vein does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454

Boster Scientific Support

Answered: 2013-09-02

Size

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PB9429
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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