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MKI67 antibody (M00254-9) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 18 Publications

m00254 9 ki67 primary antibodies fcm testing 8

Flow Cytometry analysis of Jurkat cells using anti-Ki67 antibody (M00254-9).
Overlay histogram showing Jurkat cells stained with M00254-9 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ki67 Antibody (M00254-9, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Ki67 Antibody Picoband® (monoclonal, 5C7)

Name: Anti-Ki67 Antibody Picoband® (monoclonal, 5C7)
Brand: Boster Bio
SKU: M00254-9
Price: 370 USD
Availability: InStock
Rating: 80 (1 reviews)

MKI67 antibody

18 Publications|

1 Review

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Reactive Species	Host	Application
Human	Mouse	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

MKI67 antibody was positively detected by WB in:

human K562 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MKI67 antibody was positively detected by IHC in:

human lung cancer tissue, human lymphomas tissue, human liver cancer tissue, human esophageal squamous carcinoma tissue, human cervical cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MKI67 antibody was positively detected by ICC/IF in:

A431 cell

MKI67 antibody was positively detected by FC in:

Jurkat cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-Ki67 Antibody Picoband® (monoclonal, 5C7)
View all MKI67 Antibodies

Form

Lyophilized

Uniprot

P46013

Gene ID

4288

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

5C7

Isotype

Mouse IgG2b

Immunogen

E. coli-derived human Ki67 recombinant protein (Position: K2860-I3256).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

358 kDa

Calculated Molecular Weight

358.7 kDa

Background of MKI67

Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Cite This Product

Anti-Ki67 Antibody Picoband® (monoclonal, 5C7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254-9)

Protocols

Western Blot protocol for MKI67 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MKI67 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MKI67 antibody

Review Protocol

Flow Cytometry (FC) protocol for MKI67 antibody

Review Protocol

Publications

CD168+ macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A/β-catenin/YAP1 axis

Published: 2023-05-12

Journal: iScience

Application: IHC,IF,WB

Impact Factor: 5.80

Species: Human

PMID: 37275516

SKU	M00254-9
Application	Immunohistochemistry
Sample	HepG2 subcutaneous xenograft in nude mice
Sample Processing Description	HepG2 cells were expanded in culture and subcutaneously implanted into nude mice. After 2 weeks of tumor formation, tumor tissues were harvested, fixed in 4% formaldehyde for 48 hours, and then processed for paraffin embedding and sectioning.
Other Reagents	Goat serum, DAB chromogen solution
Primary Antibody	Ki67 Antibody Picoband® (monoclonal, 5C7)
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Two-step IHC detection kit
Secondary Incubation	30 min in 37℃
Detection	Image system: Leica DM2500
Results Summary	Ki67 is a key marker of cell proliferation with high specificity, as it is expressed only in actively dividing cells and has a very short half-life, allowing accurate assessment of the proliferation index at the time of sampling. It is widely used in tumor prognosis evaluation (e.g., high Ki67 index in breast cancer is often associated with poor prognosis) and assessment of tissue regenerative activity. In this experiment, clear and distinct nuclear positivity was observed with well-defined staining.

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Anti-Ki67 Antibody Picoband® (monoclonal, 5C7)

MKI67 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

CD168+ macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A/β-catenin/YAP1 axis

Circular RNA Calm4 Regulates Hypoxia-Induced Pulmonary Arterial Smooth Muscle Cells Pyroptosis via the Circ-Calm4/miR-124-3p/PDCD6 Axis

Nanocrystallized Oleanolic Acid Better Inhibits Proliferation, Migration and Invasion in Intracranial Glioma via Caspase-3 Pathway

Moderate hypoxia modulates ABCG2 to promote the proliferation of mouse spermatogonial stem cells by maintaining mild ROS levels

Implication of myeloid differentiation factor 88 inhibitor TJ-M2010-5 for therapeutic intervention of hepatocellular carcinoma

Novel fusion cells derived from tumor cells expressing the heterologous α-galactose epitope and dendritic cells effectively target cancer

miR‑107‑5p promotes tumor proliferation and invasion by targeting estrogen receptor‑α in endometrial carcinoma

YAP signaling in gastric cancer-derived mesenchymal stem cells is critical for its promoting role in cancer progression

3D bioprinted glioma stem cells for brain tumor model and applications of drug susceptibility

Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling

Association of down-regulation of CD109 expression with up-expression of Smad7 in pathogenesis of psoriasis

Controllable organization and high throughput production of recoverable 3D tumors using pneumatic microfluidics

Restoration of microRNA‑218 increases cellular chemosensitivity to cervical cancer by inhibiting cell‑cycle progression

Effects of inhibition of hedgehog signaling on cell growth and migration of uveal melanoma cells

RPRD1B promotes tumor growth by accelerating the cell cycle in endometrial cancer

Balanced Tiam1-Rac1 and RhoA Drives Proliferation and Invasion of Pancreatic Cancer Cells

RASSF1A suppresses melanoma development by modulating apoptosis and cell-cycle progression

Cripto-1 overexpression is involved in the tumorigenesis of nasopharyngeal carcinoma

Review

Have you used Anti-Ki67 Antibody Picoband® (monoclonal, 5C7)?

1 Review For Anti-Ki67 Antibody Picoband® (monoclonal, 5C7)

IHC with Anti-Ki67 antibody (M00254-9) in HepG2 xenograft tumors showed clear nuclear staining in proliferating cells, accurately reflecting the proliferation index with low background.

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