This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
LTF antibody (A00633-1) performs well in ELISA, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
Filter:
IF analysis of LTF using anti-LTF antibody (A00633-1).
LTF was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-LTF Antibody (A00633-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
LTF was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-LTF Antibody (A00633-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Bundle Save
Anti-Lactoferrin/LTF Antibody Picoband®
LTF antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, WB |
Tested Applications
LTF antibody was positively detected by WB in:
human hepatocellular carcinoma paracancerous tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LTF antibody was positively detected by IHC in:
human prostatic cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
LTF antibody was positively detected by IF in:
human prostatic cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunofluorescence
5μg/ml
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Mouse
Recommend
Rat
Recommend
Product Information
Product Name
Anti-Lactoferrin/LTF Antibody Picoband®
View all LTF Antibodies
View all LTF Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Lactoferrin recombinant protein (Position: D529-K710).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
85 kDa
Calculated Molecular Weight
78.2 kDa
Background of LTF
Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides.
Cite This Product
Anti-Lactoferrin/LTF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00633-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
88%
Moderate Homology
Rabbit
76%
Moderate Homology
Dog
75%
Moderate Homology
Mouse
74%
Moderate Homology
Cow
73%
Moderate Homology
Sheep
72%
Moderate Homology
Rat
66%
Low homology
Zebrafish
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LTF antibody
Immunohistochemistry (IHC) protocol for LTF antibody
Immunofluorescence (IF) protocol for LTF antibody
Publications
Application:
Species:
P-Rex1 controls phagocytosis and the killing of bacteria by murine neutrophils independently of its catalytic activity
Published: 2025-09-30
Journal: Frontiers in Immunology
Application: WB
Impact Factor: 5.90
Species: Mouse
PMID: 41098722
Identification and experimental validation of ferroptosis-related gene lactotransferrin in age-related hearing loss
Published: 2024-01-11
Journal: Frontiers in Aging Neuroscience
Application: WB,IF
Impact Factor: 4.10
Species: Mouse
PMID: 38282692
Review
Have you used Anti-Lactoferrin/LTF Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-Lactoferrin/LTF Antibody Picoband®
Very Clean Anti-Lactoferrin/LTF Antibody Picoband
Source: Biocompare.com
| SKU | A00633-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse Neutrophils |
| Primary Incubation | 1:2000 1 hr |
| Blocking Agent | 5% BSA |
| Secondary Incubation | 1:3000 |
| Detection | HRP |
| Results Summary | Nicely compared total LTF in neutrophils to degranulated LTF. In the image below, Lane 1: Total LTF from mouse neutrophil cell lysates. Lane 2: degranulated LTF. Ratio 1:20. |
"Nicely detects degranulated LTF in supernatant mouse neutrophils stimulated with bacteria. Used at 1:2000 in 5% BSA."
Stephen Chetwynd
Verified customer
Submitted 2024-07-15
Product Promise
We promise Anti-Lactoferrin/LTF Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00633-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026