Overview
| Product Name |
Anti-Laminin-R (Q215) RPSA Antibody
See more RPSA products |
| Catalog# |
A01691-1 |
| Pack Size |
100ul |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-Laminin-R (Q215) RPSA Antibody catalog # A01691-1. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. |
| Cite This Product |
Anti-Laminin-R (Q215) RPSA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01691-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
IF, IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
Gene/Protein Basic Information For RPSA (Source: Uniprot.org, NCBI)
| Uniprot Id | P08865 |
|---|
| NCBI Gene Id | 3921 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | RPSA |
|---|
| Protein Name | 40S ribosomal protein SA |
|---|
| Superfamily | universal ribosomal protein uS2 family |
|---|
| Alternative Names | RPSA|37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRPNEM/1CHD4; 67 kDa laminin receptor; 67kD, ribosomal protein SA; 67LR; Colon carcinoma laminin-binding protein; LAMBR37 kDa laminin receptor; laminin binding protein; laminin receptor 1 (67kD, ribosomal protein SA); Laminin receptor 1; Laminin-binding protein precursor p40; LAMR 1 ; lamR; LAMR140S ribosomal protein SA; LBP; LBP/p40; LRP; LRP/LR; Multidrug resistance-associated protein MGr1-Ag; p40; ribosomal protein SA |
|---|
| Molecular Weight | 32854 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RPSA, check out the RPSA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for RPSA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the RPSA infographic
Our Boster Quality Guarantee for Anti-Laminin-R (Q215) RPSA Antibody covers its use in the following applications.
WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 3. Immunofluorescent staining data of RPSA using Anti-Laminin-R (Q215) RPSA Antibody (A01691-1).
IF image of A01691-1 stained A549 cells.The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Laminin-R (Q215) #A01691-1(1:100) at 10µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) FITC #BS10950 used at a 1/1000 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).
Figure 2. Immunohistochemistry validation of RPSA using Anti-Laminin-R (Q215) RPSA Antibody (A01691-1).
Immunohistochemistry (IHC) analyzes of Laminin-R (Q215) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
For more protocol information of IHC
Figure 1. Western blotting validation for Anti-Laminin-R (Q215) RPSA Antibody A01691-1
Western blot (WB) analysis of Laminin-R (Q215) polyclonal antibody at 1:500 dilution
Lane1:CT26 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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