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Product Info Summary
| SKU: | PB10094 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-67kDa Laminin Receptor/RPSA Antibody Picoband®
SKU/Catalog Number
PB10094
PB1148 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
RPSA is a small ribosomal subunit protein that, in vertebrates, is also described as the 37/67 kDa laminin receptor, linking translation machinery with laminin-binding/adhesion contexts (functional emphasis depends on localization). Assay context (per provided product description; product page not retrievable via tool at curation time): Human-reactive Picoband antibody validated for flow cytometry, IF/ICC, IHC/IHC-F, and WB to map surface vs intracellular pools. Often interpreted alongside basement-membrane markers such as Laminin (LAMA1) when evaluating ECM–cell interface biology (putative co-panel).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-67kDa Laminin Receptor/RPSA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10094)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human RPSA recombinant protein (Position: S2-S138). Human RPSA shares 100% and 99.3% amino acid (aa) sequence identity with mouse and rat RPSA, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10094 is reactive to RPSA in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
32.9 kDa
Background of RPSA
Adipose differentiation-related protein, also known as perilipin 2 (PLIN2), ADRP or adipophilin, is a protein which in humans is encoded by the ADFP gene. The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10094 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: HELA whole cell, U2OS whole cell
IHC: human mammary cancer tissue
ICC/IF: U20S cell
FCM: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of RPSA using anti-RPSA antibody (PB10094).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPSA antigen affinity purified polyclonal antibody (Catalog # PB10094) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPSA at approximately 40 kDa. The expected band size for RPSA is at 33 kDa.
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IHC analysis of RPSA using anti-RPSA antibody (PB10094).
RPSA was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPSA Antibody (PB10094) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Flow Cytometry analysis of A431 cells using anti-RPSA antibody (PB10094).
Overlay histogram showing A431 cells stained with PB10094 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPSA Antibody (PB10094,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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IF analysis of RPSA using anti-RPSA antibody (PB10094).
RPSA was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RPSA Antibody (PB10094) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-67kDa Laminin Receptor/RPSA Antibody Picoband® (PB10094)
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5 Customer Q&As for Anti-67kDa Laminin Receptor/RPSA Antibody Picoband®
Question
I am interested in to test anti-67kDa Laminin Receptor/RPSA antibody PB10094 on human skin urinary bladder for research purposes, then I may be interested in using anti-67kDa Laminin Receptor/RPSA antibody PB10094 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The products we sell, including anti-67kDa Laminin Receptor/RPSA antibody PB10094, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-14
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin urinary bladder using anti-67kDa Laminin Receptor/RPSA antibody PB10094. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-16
Question
Does anti-67kDa Laminin Receptor/RPSA antibody PB10094 work on goat Flow Cytometry with blood?
K. Li
Verified customer
Asked: 2017-08-11
Answer
Our lab technicians have not validated anti-67kDa Laminin Receptor/RPSA antibody PB10094 on goat. You can run a BLAST between goat and the immunogen sequence of anti-67kDa Laminin Receptor/RPSA antibody PB10094 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-11
Question
We are currently using anti-67kDa Laminin Receptor/RPSA antibody PB10094 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-13
Answer
The anti-67kDa Laminin Receptor/RPSA antibody (PB10094) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-13
Question
Is this PB10094 anti-67kDa Laminin Receptor/RPSA antibody reactive to the isotypes of RPSA?
H. Johnson
Verified customer
Asked: 2013-08-30
Answer
The immunogen of PB10094 anti-67kDa Laminin Receptor/RPSA antibody is E. coli-derived human RPSA recombinant protein (Position: S2-S138). Human RPSA shares 100% and 99.3% amino acid (aa) sequence identity with mouse and rat RPSA, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-08-30
