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Product Info Summary
| SKU: | M32176 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-LARGE1 Purified Monoclonal Antibody
SKU/Catalog Number
M32176
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LARGE1 Purified Monoclonal Antibody (Catalog# M32176). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-LARGE1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32176)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
LARGE-02
Isotype
Mouse IgG2b
Immunogen
Recombinant fragment of human LARGE1 (amino acids 35-142). The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase expressed mainly in the Golgi apparatus.
Cross-reactivity
Cross-reactivity with LARGE2 was not determined.
Reactive Species
M32176 is reactive to LARGE1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
88.1 kDa
Background of LARGE1
LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M32176 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-5 μg/ml. Intracellular staining.
Validation Images & Assay Conditions
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Separation of Hek293 cells transfected with LARGE1 vector and stained using anti-LARGE1 (LARGE-02) purified antibody (concentration in sample 15 µg/ml, GAM APC, red-filled) from Hek293 cells transfected with LARGE1 and unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Specific Publications For Anti-LARGE1 Purified Monoclonal Antibody (M32176)
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