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Product Info Summary
| SKU: | M01524-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Clone#RM293
SKU/Catalog Number
M01524-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Clone#RM293 (Catalog # M01524-1). Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Clone#RM293 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01524-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM293
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human LC3B.
Cross-reactivity
This antibody reacts to human LC3B (Autophagy-related protein LC3 B) (Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.
Reactive Species
M01524-1 is reactive to MAP1LC3B in Human
Calculated molecular weight
14.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01524-1 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
Immunocytochemistry (ICC): 1:100-1:200 dilution
WB: 1:100-1:200 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of cell lysates of HeLa untreated or treated with chloroquine (CHQ), using anti-LC3B rabbit monoclonal antibody (clone RM293) at a 1:200 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using anti-LC3B rabbit monoclonal antibody (clone RM293) at a 1:200 dilution.
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ICC result
Immunocytochemical staining of HeLa cells untreated or treated with chloroquine (CHQ), using anti-LC3B rabbit monoclonal Antibody Clone RM293 (red) at a 1:200 dilution. Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
Specific Publications For Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Clone#RM293 (M01524-1)
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Customer Q&As
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4 Customer Q&As for Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Clone#RM293
Question
Can you help my question with product M01524-1, anti-LC3B Rabbit Monoclonal antibody, Clone#RM293. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01524-1 anti-LC3B Rabbit Monoclonal antibody, Clone#RM293, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-21
Question
Is there a BSA free version of anti-LC3B Rabbit Monoclonal antibody, Clone#RM293 M01524-1 available?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LC3B Rabbit Monoclonal antibody, Clone#RM293 M01524-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-10
Question
We are currently using anti-LC3B Rabbit Monoclonal antibody, Clone#RM293 M01524-1 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The anti-LC3B Rabbit Monoclonal antibody, Clone#RM293 (M01524-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-12
Question
Here is the WB image, lot number and protocol we used for pheochromocytoma using anti-LC3B Rabbit Monoclonal antibody, Clone#RM293 M01524-1. Please let me know if you require anything else.
N. Wu
Verified customer
Asked: 2018-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-20
