|Product Name||Anti-TIMP4 Antibody|
|Storage & Handling||At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Metalloproteinase inhibitor 4(TIMP4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-TIMP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1078)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP4(208-224aa YRGHLPLRKEFVDIVQP), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-TIMP4 antibody, PA1078, Western blotting
All lanes: Anti TIMP4 (PA1078) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 25KD
Observed bind size: 25KD
Anti-TIMP4 antibody, PA1078, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-TIMP4 antibody, PA1078, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Metalloproteinase inhibitor 4|
|Tissue Specificity||Abundant in heart and present at low levels in many other tissues.|
|Alternative Names||Metalloproteinase inhibitor 4;Tissue inhibitor of metalloproteinases 4;TIMP-4;TIMP4;|
|Molecular Weight||25503 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9.|
|Research Areas||Angiogenesis, Cancer, Cardiovascular, Cell Biology, Ecm Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis / Ubiquitin
*You can search these to find other products in these research areas.
|Background||Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. And this gene is mapped to chromosome 3p25. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.|
Other Recommended Resources
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,