Anti-Protein NipSnap homolog 3A NIPSNAP3A Antibody

Product Name

Anti-Protein NipSnap homolog 3A NIPSNAP3A Antibody

View all NIPSNAP3A Antibodies

SKU/Catalog Number

A14892

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein NipSnap homolog 3A NIPSNAP3A Antibody (Catalog # A14892). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

NIPSNAP3A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protein NipSnap homolog 3A NIPSNAP3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14892)

Host

Rabbit

Contents

NIPSNAP3A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NIPSNAP3A antibody was raised against a 16 amino acid synthetic peptide near the center of human NIPSNAP3A. The immunogen is located within amino acids 40 - 90 of NIPSNAP3A.

Cross-reactivity

At least two isoforms of NIPSNAP3A are known to exist. NIPSNAP3A antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.

Reactive Species

A14892 is reactive to NIPSNAP3A in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

28.5 kDa

Background of NIPSNAP3A

The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low. NIPSNAP3A protein is associated with plasma membrane and partially localized in rafts. NIPSNAP proteins have been suggested to be important in vesicular transport.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14892 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NIPSNAP3A antibody can be used for detection of NIPSNAP3A by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NIPSNAP3A

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