Anti-Annexin A1/ANXA1 Antibody
|Product Name||Anti-Annexin A1/ANXA1 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Annexin A1(ANXA1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-Annexin A1/ANXA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1006)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin A1(3-24aa MVSEFLKQAWFIENEEQEYVQT).|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-Annexin A1 antibody, PA1006, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: SMMC Cell Lysate
Anti-Annexin A1 antibody, PA1006, IHC(P)
IHC(P): Human Placenta Tissue
Anti-Annexin A1 antibody, PA1006, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Annexin A1|
|Alternative Names||Annexin A1;Annexin I;Annexin-1;Calpactin II;Calpactin-2;Chromobindin-9;Lipocortin I;Phospholipase A2 inhibitory protein;p35;ANXA1;ANX1, LPC1;|
|Subcellular Localization||Nucleus . Cytoplasm . Cell projection, cilium . Basolateral cell membrane . Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. .|
|Molecular Weight||38714 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.|
*You can search these to find other products in these research areas.
|Background||Annexin I, also known as lipocortin I(Lipo1), belongs to the family of annexins. These proteins are though to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins(I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner.|
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at firstname.lastname@example.org for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact email@example.com
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to p35 antibody, annexin 1 antibody, annexin a1 antibody, annexin i antibody, annexin-1 antibody