Anti-Annexin A1/ANXA1 Antibody

Boster Bio Anti-Annexin A1/ANXA1 Antibody catalog # PA1006. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Monkey. Cited in 4 publication(s).

Product Info Summary

SKU: PA1006
Size: 100μg/vial
Reactive Species: Human, Monkey
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Annexin A1/ANXA1 Antibody

See all Annexin A1 products

SKU/Catalog Number

PA1006

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Annexin A1/ANXA1 Antibody catalog # PA1006. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Annexin A1/ANXA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1006)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin A1 (3-24aa MVSEFLKQAWFIENEEQEYVQT).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1006 is reactive to ANXA1 in Human, Monkey

Applications

PA1006 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ANXA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04083

Gene ID

301

Gene Name

ANXA1

Full Name

Annexin A1

Weight

38.714kDa

Superfamily

annexin family

Alternative Names

Annexin A1; annexin I (lipocortin I); Annexin I; Annexin-1; ANX1; ANXA1; Calpactin II; calpactin-2; chromobindin-9; Lipocortin I; LPC1; LPC1annexin-1; p35; Phospholipase A2 inhibitory protein ANXA1 ANX1, LPC1 annexin A1 annexin A1|annexin I (lipocortin I)|annexin-1|calpactin II|calpactin-2|chromobindin-9|epididymis secretory sperm binding protein|phospholipase A2 inhibitory protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANXA1, check out the ANXA1 Infographic

ANXA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANXA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1006 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma, alone and/or synergistically

Low Annexin A1 expression predicts benefit from induction chemotherapy in oral cancer patients with moderate or poor pathologic differentiation grade

Annexin A1 protein regulates the expression of PMVEC cytoskeletal proteins in CBDL rat serum-induced pulmonary microvascular remodeling

Mohamed Hg,, Idris Sb, Ahmed Mf, ??str??m An, Mustafa K, Ibrahim So, Mustafa M. Bmc Oral Health. 2015 Jul 27;15:86. Doi: 10.1186/S12903-015-0073-Z. Influence Of Type 2 Diabetes On Local Production Of Inflammatory Molecules In Adults With And Witho...

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18 Customer Q&As for Anti-Annexin A1/ANXA1 Antibody

Question

Is a blocking peptide available for product anti-Annexin A1/ANXA1 antibody (PA1006)?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

We do provide the blocking peptide for product anti-Annexin A1/ANXA1 antibody (PA1006). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-23

Question

We have tried in the past anti-Annexin A1/ANXA1 antibody for WB on cervix carcinoma erythroleukemia last year. I am using human, and I plan to use the antibody for IHC next. We need examining cervix carcinoma erythroleukemia as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

I have checked the website and datasheets of our anti-Annexin A1/ANXA1 antibody and it appears that PA1006 has been tested on human in both WB and IHC. Thus PA1006 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-18

Question

I see that the anti-Annexin A1/ANXA1 antibody PA1006 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-30

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-Annexin A1/ANXA1 antibody PA1006. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-18

Question

I would like to test anti-Annexin A1/ANXA1 antibody PA1006 on human cervix carcinoma for research purposes, then I may be interested in using anti-Annexin A1/ANXA1 antibody PA1006 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

The products we sell, including anti-Annexin A1/ANXA1 antibody PA1006, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-17

Question

We have seen staining in human cervix lung. Do you have any suggestions? Is anti-Annexin A1/ANXA1 antibody supposed to stain cervix lung positively?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

From literature cervix lung does express ANXA1. From Uniprot.org, ANXA1 is expressed in mouth mucosa, cervix lung, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ANXA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-09-05

Question

Can you help my question with product PA1006, anti-Annexin A1/ANXA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Krishna

Verified customer

Asked: 2019-07-11

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1006 anti-Annexin A1/ANXA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-11

Question

Have you mapped the epitope for PA1006?

Verified customer

Asked: 2019-05-20

Answer

Unfortunately, we do not map the epitope of our antibodies.

Boster Scientific Support

Answered: 2019-05-20

Question

Does anti-Annexin A1/ANXA1 antibody PA1006 work for WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-05-10

Answer

According to the expression profile of cervix carcinoma, ANXA1 is highly expressed in cervix carcinoma. So, it is likely that anti-Annexin A1/ANXA1 antibody PA1006 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2019-05-10

Question

Will PA1006 anti-Annexin A1/ANXA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Jha

Verified customer

Asked: 2018-06-26

Answer

You can see on the product datasheet, PA1006 anti-Annexin A1/ANXA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-06-26

Question

My team were well pleased with the WB result of your anti-Annexin A1/ANXA1 antibody. However we have observed positive staining in cervix lung nucleus using this antibody. Is that expected? Could you tell me where is ANXA1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-04-25

Answer

From what I have seen in literature, cervix lung does express ANXA1. Generally ANXA1 expresses in nucleus. Regarding which tissues have ANXA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-04-25

Question

Will anti-Annexin A1/ANXA1 antibody PA1006 work on zebrafish WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

Our lab technicians have not validated anti-Annexin A1/ANXA1 antibody PA1006 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Annexin A1/ANXA1 antibody PA1006 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-06

Question

I was wanting to use using your anti-Annexin A1/ANXA1 antibody for arachidonic acid secretion studies. Has this antibody been tested with western blotting on colo320 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-07-04

Answer

Thanks for your inquiry. This PA1006 anti-Annexin A1/ANXA1 antibody is validated on u87 cell lysate, hela cell lysate, panc cell lysate, colo320 cell lysate, smmc cell lysate, human tonsil tissue, placenta tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-07-04

Question

I was wanting to use your anti-Annexin A1/ANXA1 antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?

J. Jones

Verified customer

Asked: 2017-05-04

Answer

It shows on the product datasheet, PA1006 anti-Annexin A1/ANXA1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-05-04

Question

We are currently using anti-Annexin A1/ANXA1 antibody PA1006 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

R. Walker

Verified customer

Asked: 2015-12-28

Answer

The anti-Annexin A1/ANXA1 antibody (PA1006) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-12-28

Question

Is this PA1006 anti-Annexin A1/ANXA1 antibody reactive to the isotypes of ANXA1?

N. Li

Verified customer

Asked: 2014-09-12

Answer

The immunogen of PA1006 anti-Annexin A1/ANXA1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin A1(3-24aa MVSEFLKQAWFIENEEQEYVQT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-09-12

Question

Is there a BSA free version of anti-Annexin A1/ANXA1 antibody PA1006 available?

V. Krishna

Verified customer

Asked: 2013-09-02

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Annexin A1/ANXA1 antibody PA1006 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-09-02

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Annexin A1/ANXA1 antibody PA1006. Let me know if you need anything else.

C. Baker

Verified customer

Asked: 2013-08-08

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-08-08

Size

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PA1006
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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