|Product Name||Anti-Annexin A1/ANXA1 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Annexin A1(ANXA1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-Annexin A1/ANXA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1006)|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin A1(3-24aa MVSEFLKQAWFIENEEQEYVQT).|
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-Annexin A1 antibody, PA1006, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: SMMC Cell Lysate
Anti-Annexin A1 antibody, PA1006, IHC(P)
IHC(P): Human Placenta Tissue
Anti-Annexin A1 antibody, PA1006, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Annexin A1|
|Alternative Names||Annexin A1;Annexin I;Annexin-1;Calpactin II;Calpactin-2;Chromobindin-9;Lipocortin I;Phospholipase A2 inhibitory protein;p35;ANXA1;ANX1, LPC1;|
|Subcellular Localization||Nucleus . Cytoplasm . Cell projection, cilium . Basolateral cell membrane . Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. .|
|Molecular Weight||38714 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.|
|Background||Annexin I, also known as lipocortin I(Lipo1), belongs to the family of annexins. These proteins are though to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins(I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,