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- Table of Contents
Lcn2 antibody (PB9609) performs well in ELISA, IHC, WB, ELISA (Cap) and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).
Lipocalin 2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Lipocalin 2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Lipocalin 2/LCN2 Antibody Picoband®
Lcn2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB, ELISA (Cap) |
Tested Applications
Lcn2 antibody was positively detected by WB in:
rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Lcn2 antibody was positively detected by IHC in:
mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
ELISA (cap)
1-5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Human
Recommend
Product Information
Product Name
Anti-Lipocalin 2/LCN2 Antibody Picoband®
View all LCN2 Antibodies
View all LCN2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21-N198). Rat Lipocalin 2 shares 64.4 % and 81.1 % amino acid (aa) sequence identity with human and mouse Lipocalin 2, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated Molecular Weight
22.5 kDa
Background of Lcn2
Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.
Cite This Product
Anti-Lipocalin 2/LCN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9609)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
82%
Moderate Homology
Rabbit
65%
Low homology
Human
64%
Low homology
Monkey
63%
Low homology
Sheep
61%
Low homology
Cow
61%
Low homology
Dog
57%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Lcn2 antibody
Immunohistochemistry (IHC) protocol for Lcn2 antibody
Publications
Application:
Species:
Colchicine alleviates severe acute pancreatitis in rats by inhibiting acinar cell ferroptosis via LCN2/MAPK/ERK signaling axis
Published: 2026-05-11
Journal: EUROPEAN JOURNAL OF HISTOCHEMISTRY
Application: IHC
Impact Factor: 1.90
Species: Rat
PMID: 42112745
FPR1-dependent Pro-inflammatory Ccl4high monocytes/macrophages drive and predict sepsis-induced acute lung injury
Published: 2025-11-05
Journal: RESPIRATORY RESEARCH
Application: WB
Impact Factor: 5.00
Species: Mouse
PMID: 41194044
Inhibiting ANP32E protects against acute kidney injury by regulating autophagy via the AMPK pathway
Published: 2025-06-26
Journal: iScience
Application: WB
Impact Factor: 4.10
Species: Human
PMID: 40686617
Kidney damage following a 90-day subchronic inhalation exposure to HTP aerosol in SD rats
Published: 2025-06-24
Journal: DRUG AND CHEMICAL TOXICOLOGY
Application: IHC
Impact Factor: 1.90
Species: Rat
PMID: 40552447
Silencing HE4 alleviates the renal fibrosis in lupus nephritis mice by regulating the C3/MMPs/prss axis
Published: 2023-12-29
Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
Application: IHC
Impact Factor: 3.60
Species: Mouse
PMID: 38157023
Melittin alleviates sepsis-induced acute kidney injury by promoting GPX4 expression to inhibit ferroptosis
Published: 2023-12-27
Journal: REDOX REPORT
Application: IHC
Impact Factor: 3.80
Species: Mouse
PMID: 38149613
Specific expression and blood kinetics for relaxin 2, lipocalin 2, and tissue factor pathway inhibitor 2 at the canine placenta and pregnant bloods
Published: 2023-12-06
Journal: JOURNAL OF VETERINARY MEDICAL SCIENCE
Application: WB
Impact Factor: 1.20
Species: Dog
PMID: 38057091
Neutrophil gelatinase-associated lipocalin serum level: A potential noninvasive biomarker of endometriosis?
Published: 2023-10-13
Journal: MEDICINE
Application: IHC
Impact Factor: 1.60
Species: Human
PMID: 37832065
Up-regulation of LCN2 in the anterior cingulate cortex contributes to neural injury-induced chronic pain
Published: 2023-06-08
Journal: Frontiers in Cellular Neuroscience
Application: IF
Impact Factor: 5.30
Species: Mouse
PMID: 37362002
Recombinant FGF21 Attenuates Polychlorinated Biphenyl-Induced NAFLD/NASH by Modulating Hepatic Lipocalin-2 Expression
Published: 2022-08-10
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 6.21
Species: Human,Mouse
PMID: 36012166
Maresin conjugates in tissue regeneration-1 suppresses ferroptosis in septic acute kidney injury
Published: 2021-12-27
Journal: Cell and Bioscience
Application: WB
Impact Factor: 7.13
Species: Human,Mouse
PMID: 34961563
Inhibition of plasma kallikrein–kinin system to alleviate renal injury and arthritis symptoms in rats with adjuvant-induced arthritis
Published: 2018-01-05
Journal: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
Application: IHC
Impact Factor: 2.02
Species: Rat
PMID: 29303013
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Product Promise
We promise Anti-Lipocalin 2/LCN2 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB, ELISA (Cap). If it doesn't perform as promised, we will refund the product. Learn more
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PB9609
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026