Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL5/ENA-78. 96wells/kit, with removable strips. Cited in 1 publication(s).
Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
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|Product Name||Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine™
See all Cxcl5 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Ena-78/CXCL5 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Ena-78/CXCL5 tissue specificity:|
|Cite This Product||Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0919)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: V45-A118|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Cxcl5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||20311|
|Species Of This Entry||Mouse|
|Protein Name||C-X-C motif chemokine 5|
|Superfamily||intercrine alpha (chemokine CxC) family|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Citrullination; Acetylation; Demethylation; Reduction|
|Gene Location||5 E1|5 44.78 cM, on Chromasome 5, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Cxcl5 (Source: Uniprot.org, NCBI)
|Protein Function||May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP- 2(9-78) attract neutrophils and are involved in neutrophil activation.|
|Related Diseases||Inflammation; Neoplasms; Malignant Neoplasms; Tumor Angiogenesis; Inflammatory Response; Tissue Adhesions; Pathologic Neovascularization; Carcinoma; Neoplasm Metastasis; Arthritis; Pneumonia; Rheumatoid Arthritis; Cholangiocarcinoma; Infective Disorder; Fibrosis; Cell Invasion; Colitis; Lung Neoplasms; Cell Transformation, Neoplastic|
|Related Pathways||Pathogenesis; Angiogenesis; Secretion; Chemotaxis; Inflammatory Response; Chemokine Production; Cell Migration; Immune Response; Cell Proliferation; Cell Adhesion; Cell Growth; Neutrophil Chemotaxis; Localization; Tube Formation; Chemokine Secretion; Regeneration; Neutrophil Activation; Reverse Transcription; Wound Healing; Virulence|
|Background||C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.|
|Scientific References||PMID: 7622488 by Smith J.B., et al. Glucocorticoid-attenuated response genes encode intercellular mediators, including a new C-X-C chemokine.|
PMID: 12439624 by Smith J.B., et al. Cloning and genomic localization of the murine LPS-induced CXC chemokine (LIX) gene, Scyb5.
Kit Components And Assay QC Data Details Of Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0919-CAP||96-well plate precoated with anti-Mouse Cxcl5 antibody||1|
|EK0919-ST||lyophilized recombinant Mouse Cxcl5 standard||10ng/tube|
|EK0919-DA||biotinylated anti-Mouse Cxcl5 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0919 Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 1
|Increased neutrophil infiltration, IL-1 production and a SAPHO syndrome-like phenotype in PSTPIP2-deficient mice||PubMed ID 25602062|
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