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Product Info Summary
| SKU: | A00452 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA (Cap) |
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Product info
Product Name
Anti-Lipocalin 2/LCN2 Antibody
SKU/Catalog Number
A00452
Size
100 μg/vial
Form
Lyophilized
Description
Lcn2/NGAL is an iron-trafficking lipocalin that supports innate defense by binding siderophore–iron complexes and is also discussed in renal injury/inflammation settings (context dependent). Assay context: mouse-reactive antibody tested for capture ELISA (per provided product description); can be paired with quantitative measurement using LCN2 ELISA when designing species-matched or cross-reactivity control experiments (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lipocalin 2/LCN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00452)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00452 is reactive to Lcn2 in Mouse
Calculated molecular weight
22.9 kDa
Background of Lcn2
Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00452 is guaranteed for ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Capture (Matched Antibody Pair), 2-8μg/ml
Validation Images & Assay Conditions
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Sandwich ELISA - Recombinant mouse Lipocalin 2 protein standard curve.
Use in combination with reagents from Mouse Lipocalin 2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0854).
Specific Publications For Anti-Lipocalin 2/LCN2 Antibody (A00452)
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