Mouse KIM1/TIM1 PicoKine® Quick ELISA Kit

TIM-1/KIM-1/HAVCR ELISA kit, 90-minute one-step protocol

Mouse KIM1/TIM1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.

Product Info Summary

SKU: FEK0880
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Assay Time: 90 Minutes

Product Name

Mouse KIM1/TIM1 PicoKine® Quick ELISA Kit

See all TIM-1/KIM-1/HAVCR products

SKU/Catalog Number

FEK0880

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse KIM1/TIM1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse KIM1/TIM1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0880)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y22-T212

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0880 is reactive to Havcr1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Application Guarantee

FEK0880 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TIM-1/KIM-1/HAVCR

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.

Kit Components

Catalog numberDescription QuantityVolume
FEK0880-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0880-STMouse TIM-1/KIM-1/HAVCR Standard 2 10 ng/tube
FEK0880-CMouse Havcr1 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0490.1440.2210.3710.6561.1431.8032.355

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8329686379265805
Standard deviation6.4716.2836.256.9514.0537.84
CV (%)7.8%5.5%4.2%8.8%5.3%4.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TIM-1/KIM-1/HAVCR in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 183919199913.193.5%
Sample 229626924127727113.815.1%
Sample 386372282679780242.515.3%
*number of samples for each test n=16.

Gene/Protein Information For Havcr1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q5QNS5

Gene ID

171283

Gene Name

Havcr1

Full Name

Hepatitis A virus cellular receptor 1 homolog

Weight

33.361kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1 Havcr1|AI503787, KIM-, KIM-1, TIM-, TIM-1, Tim, Tim1, Timd1|hepatitis A virus cellular receptor 1|hepatitis A virus cellular receptor 1 homolog|HAVcr-1|T-cell immunoglobulin and mucin domain containing 1|T-cell immunoglobulin mucin receptor 1|TIMD-1|kidney injury molecule 1|t cell immunoglobulin and mucin domain-containing protein 1|t cell membrane protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Havcr1, check out the Havcr1 Infographic

Havcr1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Havcr1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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