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Product Info Summary
| SKU: | A01306 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-TIM 1/HAVCR1 Antibody Picoband®
SKU/Catalog Number
A01306
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TIM 1/HAVCR1 Antibody Picoband® catalog # A01306. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. HAVCR1 (KIM1/TIM1) is described as kidney injury molecule 1, with evidence cited for homophilic Tim–Tim interactions and for HAVCR1 serving as a receptor enabling viral infection in a cellular model. Assay context: antibody validated for ELISA and WB; reactive to HAVCR1 in human/mouse/rat; immunogen is E. coli–derived human TIM1 recombinant protein (Q58–K289) with reported observed MW ~55 kDa and stated no cross-reactivity. Often interpreted as a kidney injury/epithelial injury-associated readout (putative) and can be paired with intracellular stress/adaptation nodes such as ATG5 or CRYAB (putative); quantitative workflows may combine soluble-protein measurement (see ELISA testing) with transcript-level readouts via qPCR (putative), and mechanistic entry studies can be modeled using reporter cell lines (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIM 1/HAVCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01306)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TIM 1 recombinant protein (Position: Q58-K289).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01306 is reactive to HAVCR1 in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
39.3 kDa
Background of HAVCR1
KIM1 (KIDNEY INJURY MOLECULE 1), also known as HAVCR1, HAVCR or TIM1, is a protein that in humans is encoded by the KIM1 gene. The KIM1 gene is mapped to 5q33.3. Biochemical, mutational, and cell adhesion analyses confirm that Tim1 is capable of homophilic Tim-Tim interactions. The features identified in murine KIM1 are conserved in human KIM1. The KIM1 protein is indeed a receptor for the virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse Tim1, Tim1 is expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression of KIM1 during mouse T-cell differentiation leads to production of the Th2-type cytokine Il4, but not the Th1-type cytokine Ifng. KIM1-expressing epithelial cells internalized apoptotic bodies, and Kim1 is directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and in porcine and canine epithelial cell lines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01306 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
, Human, Mouse, Rat
ELISA (Cap), 1-5μg/ml, -
Positive Control
WB: human 293T whole cell, human HepG2 whole cel, human A549 whole cell, human CACO-2 whole cell, rat kidney tissue, rat testis tissue, mouse kidney tissue, mouse testis tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HAVCR1 using anti-HAVCR1 antibody (A01306).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAVCR1 antigen affinity purified polyclonal antibody (A01306) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HAVCR1 at approximately 55 kDa. The expected band size for HAVCR1 is at 39 kDa.
Specific Publications For Anti-TIM 1/HAVCR1 Antibody Picoband® (A01306)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-TIM 1/HAVCR1 Antibody Picoband®
Question
My question regarding product A01306, anti-TIM 1/HAVCR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01306 anti-TIM 1/HAVCR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-09
Question
Is a blocking peptide available for product anti-TIM 1/HAVCR1 antibody (A01306)?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
We do provide the blocking peptide for product anti-TIM 1/HAVCR1 antibody (A01306). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-08
Question
I was wanting to use your anti-TIM 1/HAVCR1 antibody for ELISA for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
You can see on the product datasheet, A01306 anti-TIM 1/HAVCR1 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-08
Question
We have observed staining in human adult mammalian kidney. Any tips? Is anti-TIM 1/HAVCR1 antibody supposed to stain adult mammalian kidney positively?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
From what I have seen in literature adult mammalian kidney does express HAVCR1. From what I have seen in Uniprot.org, HAVCR1 is expressed in adult mammalian kidney, liver, kidney, cervix carcinoma, among other tissues. Regarding which tissues have HAVCR1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 9658108, 15372022
Boster Scientific Support
Answered: 2019-10-23
Question
My colleagues were content with the WB result of your anti-TIM 1/HAVCR1 antibody. However we have observed positive staining in cervix carcinoma membrane using this antibody. Is that expected? Could you tell me where is HAVCR1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
From what I have seen in literature, cervix carcinoma does express HAVCR1. Generally HAVCR1 expresses in membrane. Regarding which tissues have HAVCR1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 9658108, 15372022
Boster Scientific Support
Answered: 2019-07-10
Question
We are currently using anti-TIM 1/HAVCR1 antibody A01306 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The anti-TIM 1/HAVCR1 antibody (A01306) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-14
Question
We are interested in to test anti-TIM 1/HAVCR1 antibody A01306 on human liver for research purposes, then I may be interested in using anti-TIM 1/HAVCR1 antibody A01306 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The products we sell, including anti-TIM 1/HAVCR1 antibody A01306, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-09
Question
Will A01306 anti-TIM 1/HAVCR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
It shows on the product datasheet, A01306 anti-TIM 1/HAVCR1 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-TIM 1/HAVCR1 antibody A01306. Let me know if you need anything else.
J. Parker
Verified customer
Asked: 2018-06-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-08
Question
We ordered your anti-TIM 1/HAVCR1 antibody for WB on liver in a previous experiment. I am using human, and We are going to use the antibody for ELISA next. I was wanting to use examining liver as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
B. Jones
Verified customer
Asked: 2018-05-10
Answer
I took a look at the website and datasheets of our anti-TIM 1/HAVCR1 antibody and I see that A01306 has been tested on human in both WB and ELISA. Thus A01306 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-10
Question
Is there a BSA free version of anti-TIM 1/HAVCR1 antibody A01306 available?
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TIM 1/HAVCR1 antibody A01306 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-21
Question
Is this A01306 anti-TIM 1/HAVCR1 antibody reactive to the isotypes of HAVCR1?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
The immunogen of A01306 anti-TIM 1/HAVCR1 antibody is E. coli-derived human TIM 1 recombinant protein (Position: Q58-K289). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-16
Question
I see that the anti-TIM 1/HAVCR1 antibody A01306 works with ELISA, what is the protocol used to produce the result images on the product page?
K. Taylor
Verified customer
Asked: 2016-10-10
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-10-10
Question
Does anti-TIM 1/HAVCR1 antibody A01306 work for ELISA with liver?
D. Kulkarni
Verified customer
Asked: 2013-11-13
Answer
According to the expression profile of liver, HAVCR1 is highly expressed in liver. So, it is likely that anti-TIM 1/HAVCR1 antibody A01306 will work for ELISA with liver.
Boster Scientific Support
Answered: 2013-11-13
Question
See below the WB image, lot number and protocol we used for liver using anti-TIM 1/HAVCR1 antibody A01306. Please let me know if you require anything else.
L. Krishna
Verified customer
Asked: 2013-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-21
