Anti-TIM 1/HAVCR1 Antibody Picoband™

TIM-1/KIM-1/HAVCR antibody

Boster Bio Anti-TIM 1/HAVCR1 Antibody Picoband™ catalog # A01306. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: A01306
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-TIM 1/HAVCR1 Antibody Picoband™

View all TIM-1/KIM-1/HAVCR Antibodies

SKU/Catalog Number

A01306

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TIM 1/HAVCR1 Antibody Picoband™ catalog # A01306. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TIM 1/HAVCR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01306)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human TIM 1 recombinant protein (Position: Q58-K289).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01306 is reactive to HAVCR1 in Human

Applications

A01306 is guaranteed for ELISA, WB Boster Guarantee

Background of TIM-1/KIM-1/HAVCR

KIM1 (KIDNEY INJURY MOLECULE 1), also known as HAVCR1, HAVCR or TIM1, is a protein that in humans is encoded by the KIM1 gene. The KIM1 gene is mapped to 5q33.3. Biochemical, mutational, and cell adhesion analyses confirm that Tim1 is capable of homophilic Tim-Tim interactions. The features identified in murine KIM1 are conserved in human KIM1. The KIM1 protein is indeed a receptor for the virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse Tim1, Tim1 is expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression of KIM1 during mouse T-cell differentiation leads to production of the Th2-type cytokine Il4, but not the Th1-type cytokine Ifng. KIM1-expressing epithelial cells internalized apoptotic bodies, and Kim1 is directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and in porcine and canine epithelial cell lines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For HAVCR1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q96D42

Gene ID

26762

Gene Name

HAVCR1

Full Name

Hepatitis A virus cellular receptor 1

Weight

39.25kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1 HAVCR1 CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1 hepatitis A virus cellular receptor 1 hepatitis A virus cellular receptor 1|T cell immunoglobin domain and mucin domain protein 1|T-cell immunoglobulin mucin family member 1|T-cell immunoglobulin mucin receptor 1|T-cell membrane protein 1|kidney injury molecule 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HAVCR1, check out the HAVCR1 Infographic

HAVCR1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HAVCR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01306 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu Y,Feng Q,Miao J,Wu Q,Zhou S,Shen W,Feng Y,Hou FF,Liu Y,Zhou L.C-X-C motif chemokine receptor 4 aggravates renal fibrosis through activating JAK/STAT/GSK3β/β-catenin pathway.J Cell Mol Med.2020 Apr;24(7):3837-3855.doi:10.1111/jcmm.14973.Epub 2020 Mar 2.PMID:32119183;PMCID:PMC7171406.
Species: Human,Mouse
A01306 usage in article: APP:WB, SAMPLE:HKC-8 CELL, DILUTION:NA

Tryptase and TIM-1 double-positive mast cells in different stages of human chronic periodontitis

Have you used Anti-TIM 1/HAVCR1 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-TIM 1/HAVCR1 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-TIM 1/HAVCR1 Antibody Picoband™

Question

My question regarding product A01306, anti-TIM 1/HAVCR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01306 anti-TIM 1/HAVCR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-09

Question

Is a blocking peptide available for product anti-TIM 1/HAVCR1 antibody (A01306)?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

We do provide the blocking peptide for product anti-TIM 1/HAVCR1 antibody (A01306). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-08

Question

I was wanting to use your anti-TIM 1/HAVCR1 antibody for ELISA for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

You can see on the product datasheet, A01306 anti-TIM 1/HAVCR1 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-08

Question

We have observed staining in human adult mammalian kidney. Any tips? Is anti-TIM 1/HAVCR1 antibody supposed to stain adult mammalian kidney positively?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

From what I have seen in literature adult mammalian kidney does express HAVCR1. From what I have seen in Uniprot.org, HAVCR1 is expressed in adult mammalian kidney, liver, kidney, cervix carcinoma, among other tissues. Regarding which tissues have HAVCR1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 9658108, 15372022

Boster Scientific Support

Answered: 2019-10-23

Question

My colleagues were content with the WB result of your anti-TIM 1/HAVCR1 antibody. However we have observed positive staining in cervix carcinoma membrane using this antibody. Is that expected? Could you tell me where is HAVCR1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

From what I have seen in literature, cervix carcinoma does express HAVCR1. Generally HAVCR1 expresses in membrane. Regarding which tissues have HAVCR1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 9658108, 15372022

Boster Scientific Support

Answered: 2019-07-10

Question

We are currently using anti-TIM 1/HAVCR1 antibody A01306 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

The anti-TIM 1/HAVCR1 antibody (A01306) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-14

Question

We are interested in to test anti-TIM 1/HAVCR1 antibody A01306 on human liver for research purposes, then I may be interested in using anti-TIM 1/HAVCR1 antibody A01306 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

The products we sell, including anti-TIM 1/HAVCR1 antibody A01306, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-09

Question

Will A01306 anti-TIM 1/HAVCR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

It shows on the product datasheet, A01306 anti-TIM 1/HAVCR1 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-03-01

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-TIM 1/HAVCR1 antibody A01306. Let me know if you need anything else.

J. Parker

Verified customer

Asked: 2018-06-08

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-08

Question

We ordered your anti-TIM 1/HAVCR1 antibody for WB on liver in a previous experiment. I am using human, and We are going to use the antibody for ELISA next. I was wanting to use examining liver as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

B. Jones

Verified customer

Asked: 2018-05-10

Answer

I took a look at the website and datasheets of our anti-TIM 1/HAVCR1 antibody and I see that A01306 has been tested on human in both WB and ELISA. Thus A01306 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-05-10

Question

Is there a BSA free version of anti-TIM 1/HAVCR1 antibody A01306 available?

Verified Customer

Verified customer

Asked: 2017-12-21

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TIM 1/HAVCR1 antibody A01306 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-12-21

Question

Is this A01306 anti-TIM 1/HAVCR1 antibody reactive to the isotypes of HAVCR1?

Verified Customer

Verified customer

Asked: 2017-08-16

Answer

The immunogen of A01306 anti-TIM 1/HAVCR1 antibody is E. coli-derived human TIM 1 recombinant protein (Position: Q58-K289). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-08-16

Question

I see that the anti-TIM 1/HAVCR1 antibody A01306 works with ELISA, what is the protocol used to produce the result images on the product page?

K. Taylor

Verified customer

Asked: 2016-10-10

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-10-10

Question

Does anti-TIM 1/HAVCR1 antibody A01306 work for ELISA with liver?

D. Kulkarni

Verified customer

Asked: 2013-11-13

Answer

According to the expression profile of liver, HAVCR1 is highly expressed in liver. So, it is likely that anti-TIM 1/HAVCR1 antibody A01306 will work for ELISA with liver.

Boster Scientific Support

Answered: 2013-11-13

Question

See below the WB image, lot number and protocol we used for liver using anti-TIM 1/HAVCR1 antibody A01306. Please let me know if you require anything else.

L. Krishna

Verified customer

Asked: 2013-08-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-08-21

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A01306

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

ELISA Kits

ELISA Kits

More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

Image

Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit

Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product
A01306
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.