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Product Info Summary
| SKU: | M01106-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Liver Arginase ARG1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01106-2
BM3973 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Liver Arginase ARG1 Rabbit Monoclonal Antibody catalog # M01106-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Liver Arginase ARG1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01106-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Liver Arginase
Reactive Species
M01106-2 is reactive to ARG1 in Human
Observed Molecular Weight
38 kDa
Calculated molecular weight
34.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01106-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
Positive Control
WB: human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP)
IHC: human liver tissue
Validation Images & Assay Conditions
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Western blot analysis of ARG1 using anti-ARG1 antibody (M01106-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 antigen affinity purified monoclonal antibody (Catalog # M01106-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG1 at approximately 38 kDa. The expected band size for ARG1 is at 35 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human liver, using Liver Arginase Antibody.
Specific Publications For Anti-Liver Arginase ARG1 Rabbit Monoclonal Antibody (M01106-2)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Liver Arginase ARG1 Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Liver Arginase Rabbit Monoclonal antibody M01106-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
Does M01106-2 anti-Liver Arginase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
As indicated on the product datasheet, M01106-2 anti-Liver Arginase Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-30
Question
Is a blocking peptide available for product anti-Liver Arginase Rabbit Monoclonal antibody (M01106-2)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
We do provide the blocking peptide for product anti-Liver Arginase Rabbit Monoclonal antibody (M01106-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-27
Question
My colleagues were happy with the WB result of your anti-Liver Arginase Rabbit Monoclonal antibody. However we have seen positive staining in blood cytoplasm. using this antibody. Is that expected? Could you tell me where is ARG1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
From what I have seen in literature, blood does express ARG1. Generally ARG1 expresses in cytoplasm. Regarding which tissues have ARG1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3174433
Erythroblast, Pubmed ID: 14574404
Liver, Pubmed ID: 2241902, 3540966, 24275569
Liver, and Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-17
Question
Is this M01106-2 anti-Liver Arginase Rabbit Monoclonal antibody reactive to the isotypes of ARG1?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The immunogen of M01106-2 anti-Liver Arginase Rabbit Monoclonal antibody is A synthesized peptide derived from human Liver Arginase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-06
Question
I see that the anti-Liver Arginase Rabbit Monoclonal antibody M01106-2 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-08
Question
Do you have a BSA free version of anti-Liver Arginase Rabbit Monoclonal antibody M01106-2 available?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Liver Arginase Rabbit Monoclonal antibody M01106-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-09
Question
See attached the WB image, lot number and protocol we used for liver using anti-Liver Arginase Rabbit Monoclonal antibody M01106-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
Will anti-Liver Arginase Rabbit Monoclonal antibody M01106-2 work for IP with liver?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
According to the expression profile of liver, ARG1 is highly expressed in liver. So, it is likely that anti-Liver Arginase Rabbit Monoclonal antibody M01106-2 will work for IP with liver.
Boster Scientific Support
Answered: 2019-06-03
Question
I was wanting to use your anti-Liver Arginase Rabbit Monoclonal antibody for IP for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
You can see on the product datasheet, M01106-2 anti-Liver Arginase Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
We need to test anti-Liver Arginase Rabbit Monoclonal antibody M01106-2 on human liver for research purposes, then I may be interested in using anti-Liver Arginase Rabbit Monoclonal antibody M01106-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
The products we sell, including anti-Liver Arginase Rabbit Monoclonal antibody M01106-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-21
Question
We have been able to see staining in human liver skeletal muscle. Any tips? Is anti-Liver Arginase Rabbit Monoclonal antibody supposed to stain liver skeletal muscle positively?
C. Johnson
Verified customer
Asked: 2018-07-31
Answer
Based on literature liver skeletal muscle does express ARG1. Based on Uniprot.org, ARG1 is expressed in liver, blood, erythroblast, liver skeletal muscle, among other tissues. Regarding which tissues have ARG1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3174433
Erythroblast, Pubmed ID: 14574404
Liver, Pubmed ID: 2241902, 3540966, 24275569
Liver, and Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-07-31
Question
We are currently using anti-Liver Arginase Rabbit Monoclonal antibody M01106-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
The anti-Liver Arginase Rabbit Monoclonal antibody (M01106-2) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-06
Question
Can you help my question with product M01106-2, anti-Liver Arginase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Lewis
Verified customer
Asked: 2015-08-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01106-2 anti-Liver Arginase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-11
Question
We ordered your anti-Liver Arginase Rabbit Monoclonal antibody for IF on erythroblast last year. I am using human, and We intend to use the antibody for WB next. We are interested in examining erythroblast as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
M. Taylor
Verified customer
Asked: 2014-09-18
Answer
I have checked the website and datasheets of our anti-Liver Arginase Rabbit Monoclonal antibody and I see that M01106-2 has been validated on human in both IF and WB. Thus M01106-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-09-18
