Human CCL7/MCP-3 PicoKine® Quick ELISA Kit

CCL7/MCP-3/MARC ELISA kit, 90-minute one-step protocol

Human CCL7/MCP-3 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Product Info Summary

SKU: FEK0443
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

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Product Name

Human CCL7/MCP-3 PicoKine® Quick ELISA Kit

See all CCL7/MCP-3/MARC products

SKU/Catalog Number

FEK0443

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CCL7/MCP-3 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CCL7/MCP-3 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0443)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-L99

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0443 is reactive to CCL7 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0443 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL7/MCP-3/MARC

Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Kit Components

Catalog numberDescription QuantityVolume
FEK0443-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0443-STHuman CCL7/MCP-3/MARC Standard 2 10 ng/tube
FEK0443-CHuman CCL7 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0190.0510.0810.1350.2360.4160.8501.725

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3517551241166506
Standard deviation2.7712.7828.162.8313.1130.87
CV (%)7.9%7.3%5.5%6.9%7.9%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL7/MCP-3/MARC in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135313641363.329.3%
Sample 21751661701691702.251.5%
Sample 351252644342947839.638.3%
*number of samples for each test n=16.

Gene/Protein Information For CCL7 (Source: Uniprot.Org, NCBI)

Uniprot ID

P80098

Gene ID

6354

Gene Name

CCL7

Full Name

C-C motif chemokine 7

Weight

11.2kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3) CCL7 FIC, MARC, MCP-3, MCP3, NC28, SCYA6, SCYA7 C-C motif chemokine ligand 7 C-C motif chemokine 7|chemokine (C-C motif) ligand 7|monocyte chemoattractant protein 3|monocyte chemotactic protein 3|small inducible cytokine A7 (monocyte chemotactic protein 3)|small-inducible cytokine A7

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL7, check out the CCL7 Infographic

CCL7 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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