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Product Info Summary
| SKU: | A00532 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-LSD1 KDM1A Antibody
SKU/Catalog Number
A00532
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LSD1 KDM1A Antibody (Catalog # A00532). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LSD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LSD1 KDM1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00532)
Host
Rabbit
Contents
LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-LSD1 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human LSD1. The immunogen is located within amino acids 40-90 of LSD1.
Reactive Species
A00532 is reactive to KDM1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
92.9 kDa
Background of KDM1
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00532 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.2 - 2 μg/mL; IHC-P: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: LSD1 A00532 (0.2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: LSD1 A00532 (0.2 μg/mL), LSD1 14-050 (1 μg/mL), beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse Tissues
Loading: 15 μg of lysates per lane.
Antibodies: LSD1 A00532 (0.2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse P815 Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: LSD1 A00532, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of LSD1 in Human Breast Carcinoma Tissue
Immunohistochemical analysis of paraffin-embedded human breast carcinoma Tissue using anti-LSD1 antibody (A00532) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of LSD1 in human breast carcinoma Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast carcinoma Tissue labeling LSD1 with A00532 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green/blue).
Specific Publications For Anti-LSD1 KDM1A Antibody (A00532)
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