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Product Info Summary
| SKU: | M01424 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, ICC, WB |
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Product info
Product Name
Anti-LYN Purified Monoclonal Antibody
SKU/Catalog Number
M01424
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LYN Purified Monoclonal Antibody (Catalog# M01424). Tested in IP, WB, ICC application(s). This antibody reacts with Rat, Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-LYN Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01424)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
LYN-01
Isotype
Mouse IgG1
Immunogen
Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238). The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues (intracellular antigen).
Reactive Species
M01424 is reactive to LYN in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
58.6 kDa
Background of LYN
Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01424 is guaranteed for IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Western blotting analysis of human LYN using mouse monoclonal antibody LYN-01 on lysates of Raji cell line and Jurkat cell line (LYN non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LYN monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LYN was detected around 55 kDa.
Specific Publications For Anti-LYN Purified Monoclonal Antibody (M01424)
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