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- Table of Contents
Facts about Tyrosine-protein kinase Lyn.
Required for the initiation of the B-cell response, but also for its down- regulation and termination. Plays an essential role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance.
Human | |
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Gene Name: | LYN |
Uniprot: | P07948 |
Entrez: | 4067 |
Belongs to: |
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protein kinase superfamily |
EC 2.7.10; EC 2.7.10.2; FLJ26625; Hck-2; JTK8; Lyn; tyrosine-protein kinase Lyn; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; v-yes-1; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog
Mass (kDA):
58.574 kDA
Human | |
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Location: | 8q12.1 |
Sequence: | 8; NC_000008.11 (55876041..56014169) |
Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
Cell membrane. Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Membrane; Lipid-anchor. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. Detected on plasma membrane lipid rafts.
The LYN marker is a flexible genetic tool used to examine gene expression and cellular dynamics. The marker can be used by researchers to analyze specific species or to study specific applications. The LYN marker can also be used in the production of genetically modified foods and other processes. Scientists from all over the world can submit their findings to the Boster website and receive credit for their research. Read on to discover several of the many valuable applications of the LYN marker.
Active and constant LYN regulates negative and positive signaling in the B cell. Lyn dysregulation is connected to the loss of tolerance B-cells, and the development of autoimmunity. Lyn's specific role isn't known. Lyn is located in the cytosol. It activates Csk by activating Cbp's phosphorylation.
In the absence of LPS, Lyn WT, Lyn UP, and Lyn D were significantly reduced. Both Lyn mSH2 and mSH3 levels were increased in response 100 ng/ml LPS. LYN KD and WT were elevated 13 and 7-fold in comparison to Lyn KD in unstimulated cells.
Lyn is part of the SFK family and shares architecture and sequence homology with other SFK proteins. Lyn's three domains are conserved: the N-terminal unique, Src homology and downstream SH3/SH2 domains. The unique domain in the N-terminal region helps to promote integration and localization within lipid floating rafts. Lyn's conformational state is controlled by the SH3 and SH2 domains.
The murine Lyn A marker is a DNA sequence that is found in a variety of tumors. The gene is distinguished by its tyrosine kinase-like action and is essential for tumor growth. It is also an indicator for the expression of gfp (green fluorescent protein) in cells. Multiple studies have been conducted to confirm the markers.
The recMVA-BAC method involves transfection of DF-1 cells using the target gene. The cells were transfected with a bacterial expression cassette, under the control of Lipofectamin(r), 2000. The DF-1 cells were then transfected by the helpervirus RFV (MOI 1). At the concentration indicated. The cells that were transfected were kept at 37°C for 24 and 48 h.
Anti-Bax is a monoclonal antibody that was developed to detect BAX in mouse, human, and rat cells. The antibody recognizes the N-terminus of BAX which is a gene that regulates the integrity of mitochondrial outer membranes. The company that manufactures this product under strict quality control, which ensures the highest level of quality and performance. The technical support provided by the company is unparalleled and its customer support is unsurpassed.
Boster Bio was founded in 1993 by the famous histologist Steven Xia, Boster Bio is a specialist in highly precise high-affinity, high-affinity, and ELISA kits for various research applications. Its antibody libraries are transparently validated for Flow Cytometry and immunofluorescence as well as ELISA. Boster Bio also stands behind its products by offering a 30 day money-back guarantee and continues to add new products to their extensive product line each month.
PMID: 3561390 by Yamanashi Y., et al. The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck.
PMID: 8125304 by Rider L.G., et al. The cDNAs encoding two forms of the LYN protein tyrosine kinase are expressed in rat mast cells and human myeloid cells.