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- Table of Contents
CAPG antibody (A04512-2) performs well in ELISA, IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of THP-1 cells using anti-CAPG antibody (A04512-2).
Overlay histogram showing THP-1 cells stained with A04512-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CAPG Antibody (A04512-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A04512-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CAPG Antibody (A04512-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CAPG Antibody Picoband®
CAPG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IP, IHC, WB |
Tested Applications
CAPG antibody was positively detected by WB in:
human THP-1 whole cell, human A431 whole cell, human Hacat whole cell, human HeLa whole cell, human NRK whole cell, mouse skin tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CAPG antibody was positively detected by IHC in:
rat lung tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CAPG antibody was positively detected by ICC/IF in:
Hela cell
CAPG antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
IP
ELISA
Human
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-CAPG Antibody Picoband®
View all CAPG Antibodies
View all CAPG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CAPG recombinant protein (Position: I65-K348).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
38 kDa
Calculated Molecular Weight
38.5 kDa
Background of CAPG
This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.
Cite This Product
Anti-CAPG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04512-2)
Protocols
Western Blot protocol for CAPG antibody
Immunohistochemistry (IHC) protocol for CAPG antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CAPG antibody
Flow Cytometry (FC) protocol for CAPG antibody
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Product Promise
We promise Anti-CAPG Antibody Picoband® performs excellently in relevant applications for ELISA, IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A04512-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026