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- Table of Contents
MAD2L2 Antibodies
AND MAD2L2 ELISA kits, MAD2L2 Proteins
Many biological assays use antibodies to detect MAD2L2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with MAD2L2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop MAD2L2 antibodies...
Designing an IHC experiment for MAD2L2? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the MAD2L2 IHC GuideMAD2L2 Infographic by Boster Bio
All MAD2L2 Products
Product
Figures
Specificity
Applications
Price
Human
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A02357-4
100 µg/vial
Cat # A02357-4
100 µg/vial
Human, Mouse, Rat
Flow Cytometry, IP, IF, IHC, ICC, WB
$370
Cat # M02357
100 μl
Cat # M02357
100 μl
MAD2L2 Overview
Facts about Mitotic spindle assembly checkpoint protein MAD2B.
Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2)
Adapter protein able to interact with various proteins and involved in various biological processes (PubMed:11459825, PubMed:11459826, PubMed:17719540, PubMed:17296730, PubMed:19443654, PubMed:29656893). Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the next polymerase shifting in translesion DNA synthesis (PubMed:20164194).
Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions (PubMed:20164194). Component of the shieldin complex, which has an essential role in repair of DNA double-stranded breaks (DSBs) (PubMed:29656893).
Gene Information
| Human | |
|---|---|
| Gene Name: | MAD2L2 |
| Uniprot: | Q9UI95 |
| Entrez: | 10459 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
hREV7; MAD2 mitotic arrest deficient-like 2 (yeast); MAD2BREV7 homolog; MAD2-like protein 2; Mitotic arrest deficient 2-like protein 2; mitotic arrest deficient homolog-like 2; mitotic spindle assembly checkpoint protein MAD2B; REV7yeast, homolog)-like 2
Molecular Weight
Mass (kDA):
24.334 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 1p36.22 |
| Sequence: | 1; NC_000001.11 (11674480..11691621, complement) |
Tissue Specificity
Ubiquitously expressed.
Subcellular Localizations
Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Chromosome. Recruited to sites of chromosomal double-stranded breaks during G1 and S phase of the cell cycle.
Diseases
- Malignant Neoplasms
- Neoplasms
- Carcinoma
- Aneuploidy
- Malignant Neoplasm Of Breast
- Renal Cell Carcinoma
- Mammary Neoplasms
- Cell Transformation, Neoplastic
- Cytogenetic Abnormality
- Instabilities, Chromosomal
- Kidney Neoplasm
- Trisomy
- Glioma
Interacting Proteins
- ADAM9
- CAMK2B
- CDC20
- CDC27
- CHAMP1
- FZR1
- IKZF1
- KCTD9
- REL
- REV3L
Pathways
- Cell Cycle
- Mitosis
- Spindle Checkpoint
- Spindle Assembly
- Anaphase
- Spindle Assembly Checkpoint
- Chromosome Segregation
- Cell Cycle Arrest
- Translesion Synthesis
- Metaphase
- Meiosis
- Dna Replication
- Meiosis I
PTMs
- Phosphorylation
- Ubiquitination
Research Areas
- Cell Cycle and Replication
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MAD2L2
bosterbio.com/bosterbio-gene-info-cards/MAD2L2
References
PMID: 10527948 by Nelson K.K., et al. Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2-beta.
PMID: 10366450 by Cahill D.P., et al. Characterization of MAD2B and other mitotic spindle checkpoint genes.