Anti-MAK10 NAA35 Antibody

Product Name

Anti-MAK10 NAA35 Antibody

View all MAK10 Antibodies

SKU/Catalog Number

A12206

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MAK10 NAA35 Antibody (Catalog # A12206). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

MAK10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-MAK10 NAA35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12206)

Host

Rabbit

Contents

MAK10 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MAK10 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human MAK10. The immunogen is located within amino acids 190 - 240 of MAK10.

Reactive Species

A12206 is reactive to MAK10 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

83.6 kDa

Background of MAK10

The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12206 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MAK10 antibody can be used for detection of MAK10 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information MAK10

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