Product Info Summary
| SKU: | M02571 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB, FACS |
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Product info
Product Name
Anti-MBD2/MBD3 Antibody (Monoclonal, ABM14A8)
SKU/Catalog Number
M02571
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-MBD2/MBD3 Antibody (Monoclonal, ABM14A8) (Catalog# M02571). Tested in IHC, FACS, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-MBD2/MBD3 Antibody (Monoclonal, ABM14A8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02571)
Host
Mouse
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABM14A8
Isotype
IgG1 Kappa
Immunogen
A partial length recombinant MBD3 protein (amino acids 1-290)
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02571 is reactive to MBD3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.8 kDa
Background of MBD3
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02571 is guaranteed for IHC, WB, FACS Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
IHC: 5 µg/ml, FACS analysis: 0.5 µg/10^6 cells
Validation Images & Assay Conditions
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Immunohistochemical analysis of MBD3 in Squamous cell carcinoma of Lungs using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.
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Immunohistochemical analysis of MBD3 in Squamous cell carcinoma of Lungs using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.
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Immunohistochemical analysis of MBD3 in human Colon tissue using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.
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Immunohistochemical analysis of MBD3 in human Spleen tissue using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.
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Immunohistochemical analysis of MBD3 in human Testis tissue using MBD3 antibody (Clone: ABM14A8) at 10 μg/ml.
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Immunohistochemical analysis of MBD3 in human Kidney tissue using MBD3 antibody (Clone: ABM14A8) at 10 μg/ml.
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Immunohistochemical analysis of MBD3 in human Testis tissue using MBD3 antibody (Clone: ABM14A8) at 10 μg/ml.
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Immunohistochemical analysis of MBD3 in human breast tissue using MBD3 antibody (Clone: ABM14A8) at 5 μg/ml.
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Western blot analysis of MBD3 (and MBD2). Anti-MBD3 (and MBD2) antibody (Clone: ABM14A8) was used at 2 μg/ml on A431, K562, A375 and HEK293 lysates.
Specific Publications For Anti-MBD2/MBD3 Antibody (Monoclonal, ABM14A8) (M02571)
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