Chinese Hamster IGF-2 ELISA Kit PicoKine®

IGF-II/IGF2 ELISA kit for Hamster

Chinese Hamster IGF-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Chinese Igf2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Product Info Summary

SKU: EK0380-HA
Size: 96 wells/kit, with removable strips.
Reactive Species: Hamster
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

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Product Name

Chinese Hamster IGF-2 ELISA Kit PicoKine®

View all IGF-II/IGF2 ELISA kits

SKU/Catalog Number

EK0380-HA

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Chinese Hamster IGF-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Chinese Igf2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Chinese Hamster IGF-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0380-HA)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A25-E91

Sensitivity

<5 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with IGF-1.

Reactive Species

EK0380-HA is reactive to Igf2 in Hamster samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK0380-HA is guaranteed for ELISA in Hamster by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IGF-II/IGF2

Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

Kit Components

Catalog Number Description Quantity
EK0380-HA-CAP Anti-Hamster Igf2 Pre-coated 96-well strip microplate 1
EK0380-HA-ST Hamster Igf2 Standard 2 vials, 10 ng/tube
EK0380-HA-DA Hamster Igf2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0490.1120.1960.3090.5931.041.6682.171

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Chinese Hamster IGF-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4781536274345317372968
Standard deviation20.5587.55126.230.8140.7222.6
CV (%)4.35.74.66.88.17.5

Gene/Protein Information For Igf2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01346

Gene ID

24483

Gene Name

Igf2

Full Name

Insulin-like growth factor II

Weight

20.086kDa

Superfamily

insulin family

Alternative Names

C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A Igf2|IGFII, RNIGF2|insulin-like growth factor 2|insulin-like growth factor II|IGF-II|Insulin-like growth factor II (somatomedin A)|MSA|insulin-like growth factor II (IGF-II)|multiplication-stimulating activity|multiplication-stimulating polypeptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Igf2, check out the Igf2 Infographic

Igf2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Igf2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Chinese Hamster IGF-2 ELISA Kit PicoKine®

Question

Q: Can IGF-II ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

G. Scott

Verified customer

Asked: 2019-11-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-11-11

Question

Q: how to analyze ELISA data? I measured IGF-II level in plasma.

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-09-19

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

A. Jackson

Verified customer

Asked: 2019-02-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-02-22

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Garcia

Verified customer

Asked: 2016-10-05

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-10-05

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